Fig. 4From: Heme biosensor-guided in vivo pathway optimization and directed evolution for efficient biosynthesis of hemea: Heme yield after initiation codon optimization of hemH; b: Specific growth rates of SALHT at different tetracycline concentrations with heme addition c: Computer-aided design of mutants and affinity energies for FECH; d: Molecular docking model of PPIX and FECH from Bacillus subtilis; e: The heme yields of 20 strains were randomly selected from the pressure-screened FECH mutants. *0.01 < p < 0.05, **0.001 < p < 0.01, ***p < 0.001Back to article page