Fig. 5From: Heme biosensor-guided in vivo pathway optimization and directed evolution for efficient biosynthesis of hemea: Parallel fermentations to optimize heme fermentation conditions; a 1-L fermenter was used with medium volume 600 mL and pH stabilized at 7.0; b: Heme production in a 5-L fermenter. The temperature was 30 °C, the ventilation volume was 2 vvm, and the pH was stabilized at 7.0Back to article page