Metabolic engineering to improve production of 3-hydroxypropionic acid from corn-stover hydrolysate in Aspergillus species

Dai, Ziyu; Pomraning, Kyle R.; Deng, Shuang; Kim, Joonhoon; Campbell, Kristen B.; Robles, Ana L.; Hofstad, Beth A.; Munoz, Nathalie; Gao, Yuqian; Lemmon, Teresa; Swita, Marie S.; Zucker, Jeremy D.; Kim, Young-Mo; Burnum-Johnson, Kristin E.; Magnuson, Jon K.

doi:10.1186/s13068-023-02288-1

Biotechnology for Biofuels and Bioproducts

Table 3 Parent and transgenic strains of A. pseudoterreus and A. niger used in this study

From: Metabolic engineering to improve production of 3-hydroxypropionic acid from corn-stover hydrolysate in Aspergillus species

Strain name	Relevant genotype	References
	Parent strain: Aspergillus pseudoterreus ATCC32359 (AP)
APcad1Δ	cad1::hph	[28, 59]
Ap3HP2	cad::3HP[gpdAp-panD-elf3t-bapat-eno1p, gpdAp-hpdh-trpCt, ptrA, (1 copy)*]	[28]
Ap3HP6	cad::3HP[gpdAp-panD-elf3t-bapat-eno1p, gpdAp-hpdh-trpCt, ptrA, (2 copies)]	[28]
Ap3HP6/HP7 or 16	cad::3HP[gpdAp-panD-elf3t-bapat-eno1p, gpdAp-hpdh-trpCt, ptrA, (2 copies)]/3HP[gpdAp-panD-elf3t-bapat-eno1p, gpdAp-hpdh-trpCt, hph, (unknown copies)]	This work
Ap3HP6/HP-aat series	cad::3HP[gpdAp-panD-elf3t-bapat-eno1p, gpdAp-hpdh-trpCt, ptrA, (2 copies)]//*3HP-aat*** [gpdAp-panD-elf3t-bapat:eno1p, gpdAp-hpdh-trpCt, hph, tef1p:aat:pgk1t, (unknown copies)]	This work
	Parent strain: Aspergillus niger ATCC 11414 (An)
ATCC 11414	wild-type (parent)	[31, 43]
An3HP5	3HP[gpdAp-panD-elf3t-bapat-eno1p, gpdAp-hpdh-trpCt, ptrA, (unknown copies)]	This work
An3HP9	3HP[gpdAp-panD-elf3t-bapat-eno1p, gpdAp-hpdh-trpCt, ptrA, (12 copies)**]	This work
An3HP10	3HP[gpdAp-panD-elf3t-bapat-eno1p, gpdAp-hpdh-trpCt, ptrA (unknown copies)]	This work
An3HP9/aat2, 3, or 5	3HP[gpdAp-panD-elf3t-bapat-enop, gpdAp-hpdh-trpCt, ptrA, (12 copies)]/*aat[ble, tef1p-aat-pgk1t) (An3HP9/aat2*: 5 copies)]	This work
An3HP9/pyc2, 5, or 6	3HP[gpdAp-panD-elf3t-bapat-enop, gpdAp-hpdh-trpCt, ptrA, (12 copies)]/*pyc[ble, mbf1p-pyc-pgk1t (An3HP9/pyc*2: 8 copies)]	This work
An3HP9/pyc2/oahAΔ	3HP[gpdAp-panD-elf3t-bapat-enop, gpdAp-hpdh-trpCt, ptrA, (12 copies)]/*pyc[ble, mbf1p-pyc-pgk1t (An3HP9/pyc2: 8 copies)]/oahAΔ(oahA::hph*)	This work
An3HP9/pyc2/ald6aΔ	3HP[gpdAp-panD-elf3t-bapat-enop, gpdAp-hpdh-trpCt, ptrA, (12 copies)]/*pyc[ble, mbf1p-pyc-pgk1t (An3HP9/pyc2: 8 copies)]/ald6aΔ(ald6a::hph*)	This work
An3HP9/pyc2/ald6bΔ	3HP(gpdAp-panD-elf3t-bapat-enop, gpdAp-hpdh-trpCt, ptrA, (12 copies)]/*pyc[/ble, mbf1p-pyc-pgk1t (An3HP9/pyc2: 8 copies)]// ald6bΔ(ald6b::hph*)	This work
An3HP9/pyc2/ald3Δ	3HP(gpdAp-panD-elf3t-bapat-enop, gpdAp-hpdh-trpCt, ptrA, (12 copies)]/*pyc[/ble, mbf1p-pyc-pgk1t (An3HP9/pyc2: 8 copies)]/ald3Δ(ald3::hph*)	This work
An3HP9/pyc2/uga2Δ	3HP[gpdAp-panD-elf3t-bapat-enop, gpdAp-hpdh-trpCt, ptrA, (12 copies)]/*pyc[ble, mbf1p-pyc-pgk1t (An3HP9/pyc2: 8 copies)]//uga2Δ(uga2::hph*)	This work
An3HP9/pyc2/mct1	3HP[gpdAp-panD-elf3t-bapat-enop, gpdAp-hpdh-trpCt, ptrA, (12 copies)]pyc[/ble, mbf1p-pyc-pgk1t (An3HP9/pyc2: 8 copies)]/mct1(pmbfAp-mct1-msft, nat1)	This work
An3HP9/pyc2/ald6aΔ/3HP-6	3HP(gpdAp-panD-elf3t-bapat-enop, gpdAp-hpdh-trpCt, ptrA, (12 copies)]/pyc[ble, mbf1p-pyc-pgk1t (An3HP9/pyc2: 8 copies)]/ald6Δ(ald6a::hph)/3HP[ubi4p-panD-elf3t-bapat-ubiSp, mbfAp-hpdh-trpCt, loxP-nptII (15copies)]	This work

*Copy number estimated by Southern blotting analysis; **copy numbers estimated by whole genomic DNA sequencing

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