Functioning of a tripartite lignocellulolytic microbial consortium cultivated under two shaking conditions: a metatranscriptomic study

Table 1 Overall gene expression at different levels across strains under different shaking speeds over time

	TPM value	Level	180 rpm			60 rpm			Ratio (%)
	TPM value	Level	1d	5d	13d	1d	5d	13d	Ratio (%)
so4 (4567)	> 1000	H	61	53	35	67	25	45	1.04
	100–1000	I	1151	1873	1333	2476	3976	4216	54.83
	< 100	L	3356	2641	3199	2024	566	306	44.13
w15 (5497)	> 1000	H	62	41	49	56	40	28	0.84
	100–1000	I	3731	3556	4478	3364	4079	4956	73.26
	< 100	L	1704	1900	970	2077	1378	513	25.90
2T2.1 (23,406)	> 1000	H	128	69	62	118	63	59	0.36
	100–1000	I	1491	1742	1770	1588	1632	1780	7.12
	< 100	L	21,787	21,595	21,574	21,700	21,711	21,567	92.52

Transcripts Per Kilobase Million (TPM) values of each sample were used. Expression level: high (H), intermediate (I), and low (L). Ratio calculated as: mean of expressed gene number at a certain level/ total expressed gene number of each genome (number in brackets under the strain name). Genes with raw read numbers in each sample below 10 or with summed number in all 18 samples below 60 were treated as non-expressed

ISSN: 2731-3654