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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Simultaneous expression of an endogenous spermidine synthase and a butanol dehydrogenase from Thermoanaerobacter pseudethanolicus in Clostridium thermocellum results in increased resistance to acetic acid and furans, increased ethanol production and an increase in thermotolerance

Fig. 2

Effects of spermidine (SPD) supplementation on cell growth and fermentation products of C. thermocellum strains in defined medium without fermentation inhibitors (A and B) and in the presence of 10 mM furfural (C and D), HMF (E and F), or acetic acid (G and H). A Cell growth of JWCT02 (pyrF + pDCW89) with and without 0.5, 1.0, 2.0, and 4.0 mM spermidine. B, C, E, and G Cell growth of C. thermocellum JWCT08-1 (pyrF + bdhA) with and without 0.5, 1.0, 2.0, and 4.0 mM spermidine. D, F, and H Cellobiose consumed and fermentation products of C. thermocellum JWCT08-1 with and without 0.5, 1.0, 2.0, and 4.0 mM spermidine. Results are the mean of duplicate experiments and error bars indicate standard deviation

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