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Fig. 3 | Biotechnology for Biofuels and Bioproducts

Fig. 3

From: Simultaneous expression of an endogenous spermidine synthase and a butanol dehydrogenase from Thermoanaerobacter pseudethanolicus in Clostridium thermocellum results in increased resistance to acetic acid and furans, increased ethanol production and an increase in thermotolerance

Fig. 3

Effects of SpeE expression with the BdhA expression on cell growth and fermentation products of C. thermocellum strains in defined medium without fermentation inhibitors (A and B) and in the presence of 10 mM furfural (C and D), 12 mM HMF (E and F), or 10 mM acetic acid (G and H). Strains were grown in defined or complex medium with 5 g/L cellobiose containing 10 µg/mL thiamphenicol. A, C, E, and G Cell growth of JWCT13 (pyrF + pJGW37) and JWCT16 (pyrF + bdhA + speE) strains. B, D, F, and H Cellobiose consumed and fermentation products of JWCT13 and JWCT16 strains. JWCT13, parental strain; JWCT16, BdhA and SpeE expressing strain. Results are the mean of duplicate experiments and error bars indicate standard deviation

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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