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Table 3 Advantages and disadvantages of using biological pretreatment processes [119, 122, 138]

From: Strategies of pretreatment of feedstocks for optimized bioethanol production: distinct and integrated approaches

Advantages

Disadvantages

Low capital investment as the bacteria and fungi are easily extracted from natural sources

Long microbial pretreatment time generally requires 15–20 days for maximum delignification of biomass

Low energy requirement as it is incubated at 32 ℃ temperature hardly for 72 h

Lower content of soluble hydrolysate is obtained with an efficiency rate of 37–40%

Efficient and environmentally safe as microbes used during the process are not hazardous as in the case of chemical and physiochemical pretreatment

Tardy rate of saccharification of LCBs as 30% lignin removal has occurred during bacterial action