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Fig. 2 | Biotechnology for Biofuels and Bioproducts

Fig. 2

From: Stoichiometric balance ratio of cellobiose and gentiobiose induces cellulase production in Talaromyces cellulolyticus

Fig. 2

Inducer generation upon co-incubation of spores and avicel. a A schematic representation of experimental setup for production of potential inducer metabolite cocktail. b The transglycosylated metabolites, produced by co-incubation of 1% avicel with 1 × 109 spores, were supplemented to liquid culture for evaluating its role as potential inducers. The culture supernatant was analyzed for its effect on cellulolytic activity, whereas metabolites produced from starch spore, spore alone, and avicel alone incubations were used as control. c Effect of inducers on expression of key cellulolytic gene was performed where AVS, AVC, and SPC represent avicel and spore co-incubation, avicel alone, and spore alone, respectively. Briefly, AVS, AVC, and SPC are incubated at 50 °C for 72 h and the supernatant was utilized as inducer for its effect on growth and RT-PCR analysis

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