Fig. 5

Stoichiometric balanced ratio of cellobiose and gentiobiose for optimal cellulase induction. a A range of saccharide combinations were analyzed for their effect on secretion of cellulase enzymes. The integrative effect was observed by evaluating the enzyme cocktail for its effect on hydrolysis of pre-treated wheat straw. The experiment was performed in two technical and two biological replicates. b A minimal culture medium containing 1% avicel. The supernatant was harvested after 48 h and different cellulolytic activity was estimated using standard biochemical assay (indicated with black bar). No inducer was user negative control (indicated with gray bar). The experiment was performed in two technical and two biological replicates