Fig. 6

Submerged cultivation to evaluate effect of optimized inducer on cellulase induction. The inducers were added at the concentration of 12.5 mM cellobiose and 5 mM gentiobiose to complex culture medium containing 2.4%WB and 2.14% avicel. The supernatant was harvested after 72, 96, and 120 h and different cellulolytic activity was estimated using standard biochemical assay. No inducer was user negative control. The experiment was performed in two technical and two biological replicates. a Cumulative activities at 120 h. b Effect of inducer on time-dependent production of cellulases. The experiment was done in triplicate and standard deviation was calculated accordingly