Fig. 7

Combined effect of Inducers and cre knockout on composition of cellulase cocktail. The inducers were added at the concentration of 12.5 mM cellobiose and 5 mM gentiobiose to complex culture medium containing 2.4%WB and 2.14% avicel. Active mycelia were pre-grown in PDB for 36 h and used as inoculum. The supernatant were harvested after 5 days and concentrated using a 10 kDa centricon. No inducer was used as negative control. Total glucose released after 2 h of saccharification of pretreated wheat straw, Napier grass, sorghum, and sugarcane was estimated by the GOD–POD kit. The experiment was performed in two technical replicates