Fig. 6

Transcription factor MtAra-1 directly regulates expression of Mtlat-1. A Venn diagram analysis of transcription factor genes with significantly upregulated expression levels in WT M. thermophila grown on l-arabinose, d-xylose, or d-glucose, compared with growth in no carbon conditions. B Relative expression levels of Mtlat-1 in the mutants ΔMtara-1, ΔMtpdr-1, ΔMtpdr-2, and ΔMycth_2300935, compared with the WT after the induction by l-arabinose for 4 h. Error bars indicate the SD from at least three biological replicates. C Electrophoretic mobility shift assays of the binding of MtAra-1 to the upstream region of Mtlat-1. Each lane contained 10 ng probe and the indicated amount of the purified DNA-binding domain (nM) of MtAra-1. D Sugar consumption by strain ΔMtara-1 when grown in 1 × VMM with 2% l-arabinose. E Expression levels of genes involved in l-arabinose catabolism in the ΔMtara-1 mutant of M. thermophila when grown on l-arabinose for 4 h. XR, xylose reductase (Mycth_43671); ARD, l-arabinitol 4-dehydrogenase (Mycth_62052); LXR, l-xylulose reductase (Mycth_2302811); XKS, d-xylulose kinase (Mycth_67060)