Fig. 2
From: The Ras small GTPase RSR1 regulates cellulase production in Trichoderma reesei
Expression levels of major cellulase genes when Avicel is used as the carbon source. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect the transcriptional levels of four major cellulase genes, cbh1 (A), cbh2 (B), egl1 (C), and egl2 (D), and two major xylanase genes xyn1 (E) and xyn2 (F). Conidia of T. reesei were inoculated into MA liquid medium with 1% (w/v) Avicel as the sole carbon source. Sampling was done at 36, 48, and 60 h. The data are normalized to expression of QM6a at 36 h for each tested gene, with the sar1 gene used as an endogenous control in all samples. Values are represented as mean ± SD of the results from three independent experiments. Asterisks (*) indicate significant differences compared to the parental strain (Student’s t-test, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001)