Fig. 5
From: The Ras small GTPase RSR1 regulates cellulase production in Trichoderma reesei
Effect of the loss of rsr1 on the ACY1-cAMP-PKA pathway. A, B Intracellular cyclic adenosine monophosphate (cAMP) content of the original strain (QM6a), deletion strain (Δrsr1), and complementation strain (RC-rsr1) were measured when 1% (w/v) Avicel (A) and 2% glucose (B) were the sole carbon source, respectively. Fresh conidia were generated from hyphae in MA liquid medium and intracellular cAMP content was measured at 2 days, as described in the Methods. (C–F) Transcriptional levels of acy1 (C), pkac1 (D), pkac2 (E), and pkar1 (F) detected by RT-qPCR in QM6a, Δrsr1, and RC-rsr1 strains with 1% (w/v) Avicel as the sole carbon source. Values are represented as mean ± SD of the results from three independent experiments. Asterisks (*) indicate significant differences compared to the parental strain (Student’s t-test, ***P < 0.001; ****P < 0.0001; ns represents no significant difference)