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Fig. 6 | Biotechnology for Biofuels and Bioproducts

Fig. 6

From: The Ras small GTPase RSR1 regulates cellulase production in Trichoderma reesei

Fig. 6

Effect of ACY1-cAMP-PKA pathway on rsr1-mediated cellulase overexpression. pNPCase activity (A) and CMCase activity (B) of T. reesei QM6a, Δacy1, Δrsr1, Δrsr1Δacy1, QM6a-OEacy1 and Δrsr1-OEacy1 cultured in MA with 1% (w/v) Avicel at 2, 3, and 4 days. The transcript levels of cbh1 (C), cbh2 (D), egl1 (E), and egl2 (F) in T. reesei QM6a, Δacy1, Δrsr1, Δrsr1Δacy1, QM6a-OEacy1 and Δrsr1-OEacy1 strains cultured in MA with 1% (w/v) Avicel at 36 and 48 h. Values are represented as mean ± SD of the results from three independent experiments. Asterisks (*) indicate significant differences compared to the parental strain (Student’s t-test, *P < 0.05)

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