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  1. Microalgal strain development through genetic engineering has received much attention as a way to improve the traits of microalgae suitable for biofuel production. However, there are still some limitations in ...

    Authors: Sung-Eun Shin, Hyun Gi Koh, Nam Kyu Kang, William I. Suh, Byeong-ryool Jeong, Bongsoo Lee and Yong Keun Chang
    Citation: Biotechnology for Biofuels 2017 10:308
    Content type: Research Published on:

  2. Synechocystis sp. PCC 6803 is an attractive organism for the production of alcohols, such as isobutanol and ethanol. However, because stress against the produced alcohol is a major bar...

    Authors: Takuya Matsusako, Yoshihiro Toya, Katsunori Yoshikawa and Hiroshi Shimizu
    Citation: Biotechnology for Biofuels 2017 10:307
    Content type: Research Published on:

  3. Bioflocculation has been developed as a cost-effective and environment-friendly method to harvest multiple microalgae. However, the high production cost of bioflocculants makes it difficult to scale up. In the...

    Authors: Haipeng Guo, Chuntao Hong, Bingsong Zheng, Fan Lu, Dean Jiang and Wensheng Qin
    Citation: Biotechnology for Biofuels 2017 10:306
    Content type: Research Published on:

  4. Anaerobic gut fungi are the primary colonizers of plant material in the rumen microbiome, but are poorly studied due to a lack of characterized isolates. While most genera of gut fungi form extensive rhizoidal...

    Authors: John K. Henske, Sean P. Gilmore, Doriv Knop, Francis J. Cunningham, Jessica A. Sexton, Chuck R. Smallwood, Vaithiyalingam Shutthanandan, James E. Evans, Michael K. Theodorou and Michelle A. O’Malley
    Citation: Biotechnology for Biofuels 2017 10:305
    Content type: Research Published on:

  5. As a major lignocellulosic biomass, which represented more than half of the world’s agricultural phytomass, crop residues have been considered as feedstock for biofuel production. However, large-scale applicat...

    Authors: Hui Wang, Kashif ur Rehman, Xiu Liu, Qinqin Yang, Longyu Zheng, Wu Li, Minmin Cai, Qing Li, Jibin Zhang and Ziniu Yu
    Citation: Biotechnology for Biofuels 2017 10:304
    Content type: Research Published on:

  6. Recent studies have suggested that addition of electrically conductive biochar particles is an effective strategy to improve the methanogenic conversion of waste organic substrates, by promoting syntrophic ass...

    Authors: Carolina Cruz Viggi, Serena Simonetti, Enza Palma, Pamela Pagliaccia, Camilla Braguglia, Stefano Fazi, Silvia Baronti, Maria Assunta Navarra, Ida Pettiti, Christin Koch, Falk Harnisch and Federico Aulenta
    Citation: Biotechnology for Biofuels 2017 10:303
    Content type: Research Published on:

  7. Food waste is a large bio-resource that may be converted to biogas that can be used for heat and power production, or as transport fuel. We studied the anaerobic digestion of food waste in a staged digestion s...

    Authors: John Christian Gaby, Mirzaman Zamanzadeh and Svein Jarle Horn
    Citation: Biotechnology for Biofuels 2017 10:302
    Content type: Research Published on:

  8. Surfactants have attracted increasing interest for their capability to improve the enzymatic hydrolysis of lignocellulosic biomass. Compared to chemical surfactants, biosurfactants have a broader prospect for ...

    Authors: Jiawen Liu, Ning Zhu, Jinshui Yang, Yi Yang, Ruonan Wang, Liang Liu and Hongli Yuan
    Citation: Biotechnology for Biofuels 2017 10:301
    Content type: Research Published on:

  9. In this study, confocal Raman microspectroscopy was used to detect lipids in microalgae rapidly and non-destructively. Microalgae cells were cultured under nitrogen deficiency. The accumulation of lipids in Scene...

    Authors: Yongni Shao, Hui Fang, Hong Zhou, Qi Wang, Yiming Zhu and Yong He
    Citation: Biotechnology for Biofuels 2017 10:300
    Content type: Research Published on:

  10. C2–C4 diols classically derived from fossil resource are very important bulk chemicals which have been used in a wide range of areas, including solvents, fuels, polymers, cosmetics, and pharmaceuticals. Produc...

    Authors: Ye Zhang, Dehua Liu and Zhen Chen
    Citation: Biotechnology for Biofuels 2017 10:299
    Content type: Review Published on:

  11. A recently constructed cellulolytic Yarrowia lipolytica is able to grow efficiently on an industrial organosolv cellulose pulp, but shows limited ability to degrade crystalline cellulose. In this work, we have fu...

    Authors: Zhong-peng Guo, Sophie Duquesne, Sophie Bozonnet, Jean-Marc Nicaud, Alain Marty and Michael Joseph O’Donohue
    Citation: Biotechnology for Biofuels 2017 10:298
    Content type: Research Published on:

  12. Isoprene as the feedstock can be used to produce renewable energy fuels, providing an alternative to replace the rapidly depleting fossil fuels. However, traditional method for isoprene production could not me...

    Authors: Sumeng Wang, Zhaobao Wang, Yongchao Wang, Qingjuan Nie, Xiaohua Yi, Wei Ge, Jianming Yang and Mo Xian
    Citation: Biotechnology for Biofuels 2017 10:297
    Content type: Research Published on:

  13. New biorefinery concepts are necessary to drive industrial use of lignocellulose biomass components. Xylan recovery before enzymatic hydrolysis of the glucan component is a way to add value to the hemicellulos...

    Authors: Daniele Sporck, Felipe A. M. Reinoso, Jorge Rencoret, Ana Gutiérrez, José C. del Rio, André Ferraz and Adriane M. F. Milagres
    Citation: Biotechnology for Biofuels 2017 10:296
    Content type: Research Published on:

  14. In their quest for sustainable development and effective management of greenhouse gas emissions, our societies pursue a shift away from fossil-based resources towards renewable resources. With 95% of our curre...

    Authors: Katharina Anna Lindlbauer, Hans Marx and Michael Sauer
    Citation: Biotechnology for Biofuels 2017 10:295
    Content type: Research Published on:

  15. The genetic modification of plant cell walls has been considered to reduce lignocellulose recalcitrance in bioenergy crops. As a result, it is important to develop a precise and rapid assay for the major wall ...

    Authors: Jiangfeng Huang, Ying Li, Yanting Wang, Yuanyuan Chen, Mingyong Liu, Youmei Wang, Ran Zhang, Shiguang Zhou, Jingyang Li, Yuanyuan Tu, Bo Hao, Liangcai Peng and Tao Xia
    Citation: Biotechnology for Biofuels 2017 10:294
    Content type: Research Published on:

  16. Lignocellulosic biomass will progressively become the main source of carbon for a number of products as the Earth’s oil reservoirs disappear. Technology for conversion of wood fiber into bioproducts (wood bior...

    Authors: Pierre-Louis Bombeck, Vinay Khatri, Fatma Meddeb-Mouelhi, Daniel Montplaisir, Aurore Richel and Marc Beauregard
    Citation: Biotechnology for Biofuels 2017 10:293
    Content type: Research Published on:

  18. The efficiency of cellulolytic enzymes is important in industrial biorefinery processes, including biofuel production. Chemical methods, such as alkali pretreatment, have been extensively studied and demonstra...

    Authors: Kiyota Sakai, Saki Kojiya, Junya Kamijo, Yuta Tanaka, Kenta Tanaka, Masahiro Maebayashi, Jun-Seok Oh, Masafumi Ito, Masaru Hori, Motoyuki Shimizu and Masashi Kato
    Citation: Biotechnology for Biofuels 2017 10:290
    Content type: Research Published on:

  19. High temperature inhibits cell growth and ethanol fermentation of Saccharomyces cerevisiae. As a complex phenotype, thermotolerance usually involves synergistic actions of many genes, thereby being difficult to e...

    Authors: Pengsong Li, Xiaofen Fu, Lei Zhang, Zhiyu Zhang, Jihong Li and Shizhong Li
    Citation: Biotechnology for Biofuels 2017 10:289
    Content type: Research Published on:

  20. Tobacco is widely planted as an important nonfood economic crop throughout the world, and large amounts of tobacco wastes are generated during the tobacco manufacturing process. Tobacco and its wastes contain ...

    Authors: Wenjun Yu, Rongshui Wang, Huili Li, Jiyu Liang, Yuanyuan Wang, Haiyan Huang, Huijun Xie and Shuning Wang
    Citation: Biotechnology for Biofuels 2017 10:288
    Content type: Research Published on:

  21. The authors noticed an accidental calculation error existing in Fig. 4C of this article [1]. The given velocities of the GAT-1 transporter (in this figure only) were mistakenly listed 50-fold too high. Based on t...

    Authors: J. Philipp Benz, Ryan J. Protzko, Jonas M. S. Andrich, Stefan Bauer, John E. Dueber and Chris R. Somerville
    Citation: Biotechnology for Biofuels 2017 10:287
    Content type: Correction Published on:

    The original article was published in Biotechnology for Biofuels 2014 7:20

  22. Populus natural variants have been shown to realize a broad range of sugar yields during saccharification, however, the structural features responsible for higher sugar release from na...

    Authors: Vanessa A. Thomas, Ninad Kothari, Samarthya Bhagia, Hannah Akinosho, Mi Li, Yunqiao Pu, Chang Geun Yoo, Sivakumar Pattathil, Michael G. Hahn, Arthur J. Raguaskas, Charles E. Wyman and Rajeev Kumar
    Citation: Biotechnology for Biofuels 2017 10:292
    Content type: Research Published on:

  23. Xylans are the most abundant noncellulosic polysaccharides in lignified secondary cell walls of woody dicots and in both primary and secondary cell walls of grasses. These polysaccharides, which comprise 20–35...

    Authors: Peter J. Smith, Hsin-Tzu Wang, William S. York, Maria J. Peña and Breeanna R. Urbanowicz
    Citation: Biotechnology for Biofuels 2017 10:286
    Content type: Review Published on:

  24. The DOE BioEnergy Science Center has operated as a virtual center with multiple partners for a decade targeting overcoming biomass recalcitrance. BESC has redefined biomass recalcitrance from an observable phe...

    Authors: Paul Gilna, Lee R. Lynd, Debra Mohnen, Mark F. Davis and Brian H. Davison
    Citation: Biotechnology for Biofuels 2017 10:285
    Content type: Commentary Published on:

  25. The development of genome editing technologies offers new prospects in improving bioenergy crops like switchgrass (Panicum virgatum). Switchgrass is an outcrossing species with an allotetraploid genome (2n = 4x =...

    Authors: Jong-Jin Park, Chang Geun Yoo, Amy Flanagan, Yunqiao Pu, Smriti Debnath, Yaxin Ge, Arthur J. Ragauskas and Zeng-Yu Wang
    Citation: Biotechnology for Biofuels 2017 10:284
    Content type: Research Published on:

  26. With the discovery of interspecies hydrogen transfer in the late 1960s (Bryant et al. in Arch Microbiol 59:20–31, 1967), it was shown that reducing the partial pressure of hydrogen could cause mixed acid fermenti...

    Authors: Ayşenur Eminoğlu, Sean Jean-Loup Murphy, Marybeth Maloney, Anthony Lanahan, Richard J. Giannone, Robert L. Hettich, Shital A. Tripathi, Ali Osman Beldüz, Lee R. Lynd and Daniel G. Olson
    Citation: Biotechnology for Biofuels 2017 10:282
    Content type: Research Published on:

  28. Clostridium thermocellum is a promising microorganism for conversion of cellulosic biomass to biofuel, without added enzymes; however, the low ethanol titer produced by strains develop...

    Authors: Liang Tian, Skyler J. Perot, David Stevenson, Tyler Jacobson, Anthony A. Lanahan, Daniel Amador-Noguez, Daniel G. Olson and Lee R. Lynd
    Citation: Biotechnology for Biofuels 2017 10:276
    Content type: Research Published on:

  29. Glycoside hydrolase (GH) family 48 is an understudied and increasingly important exoglucanase family found in the majority of bacterial cellulase systems. Moreover, many thermophilic enzyme systems contain GH4...

    Authors: Roman Brunecky, Markus Alahuhta, Deanne W. Sammond, Qi Xu, Mo Chen, David B. Wilson, John W. Brady, Michael E. Himmel, Yannick J. Bomble and Vladimir V. Lunin
    Citation: Biotechnology for Biofuels 2017 10:274
    Content type: Research Published on:

  30. Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are prominent candidate biocatalysts that, together, can enable the direct biotic conversion of lignocellulosic bioma...

    Authors: Kyle Sander, Keiji G. Asano, Deepak Bhandari, Gary J. Van Berkel, Steven D. Brown, Brian Davison and Timothy J. Tschaplinski
    Citation: Biotechnology for Biofuels 2017 10:270
    Content type: Methodology Published on:

  31. Plant cell walls contribute the majority of plant biomass that can be used to produce transportation fuels. However, the complexity and variability in composition and structure of cell walls, particularly the ...

    Authors: Xiaolan Rao, Hui Shen, Sivakumar Pattathil, Michael G. Hahn, Ivana Gelineo-Albersheim, Debra Mohnen, Yunqiao Pu, Arthur J. Ragauskas, Xin Chen, Fang Chen and Richard A. Dixon
    Citation: Biotechnology for Biofuels 2017 10:266
    Content type: Research Published on:

  32. Understanding plant cell wall cross-linking chemistry and polymeric architecture is key to the efficient utilization of biomass in all prospects from rational genetic modification to downstream chemical and bi...

    Authors: Yining Zeng, Michael E. Himmel and Shi-You Ding
    Citation: Biotechnology for Biofuels 2017 10:263
    Content type: Review Published on:

  34. Genetic engineering has been effective in altering cell walls for biofuel production in the bioenergy crop, switchgrass (Panicum virgatum). However, regulatory issues arising from gene flow may prevent commercial...

    Authors: Chelsea R. Johnson, Reginald J. Millwood, Yuhong Tang, Jiqing Gou, Robert W. Sykes, Geoffrey B. Turner, Mark F. Davis, Yi Sang, Zeng-Yu Wang and C. Neal Stewart Jr.
    Citation: Biotechnology for Biofuels 2017 10:255
    Content type: Research Published on:

  35. One of the major barriers to the development of lignocellulosic feedstocks is the recalcitrance of plant cell walls to deconstruction and saccharification. Recalcitrance can be reduced by targeting genes invol...

    Authors: David Macaya-Sanz, Jin‐Gui Chen, Udaya C. Kalluri, Wellington Muchero, Timothy J. Tschaplinski, Lee E. Gunter, Sandra J. Simon, Ajaya K. Biswal, Anthony C. Bryan, Raja Payyavula, Meng Xie, Yongil Yang, Jin Zhang, Debra Mohnen, Gerald A. Tuskan and Stephen P. DiFazio
    Citation: Biotechnology for Biofuels 2017 10:253
    Content type: Research Published on:

  36. Consolidated bioprocessing (CBP) by anaerobes, such as Clostridium thermocellum, which combine enzyme production, hydrolysis, and fermentation are promising alternatives to historical economic challenges of using...

    Authors: Vanessa A. Thomas, Bryon S. Donohoe, Mi Li, Yunqiao Pu, Arthur J. Ragauskas, Rajeev Kumar, Thanh Yen Nguyen, Charles M. Cai and Charles E. Wyman
    Citation: Biotechnology for Biofuels 2017 10:252
    Content type: Research Published on:

  38. Xylan is a major hemicellulosic component in the cell walls of higher plants especially in the secondary walls of vascular cells which are playing important roles in physiological processes and overall mechani...

    Authors: Angelo G. Peralta, Sivasankari Venkatachalam, Sydney C. Stone and Sivakumar Pattathil
    Citation: Biotechnology for Biofuels 2017 10:245
    Content type: Research Published on:

  39. Auxiliary activity (AA) enzymes are produced by numerous bacterial and fungal species to assist in the degradation of biomass. These enzymes are abundant but have yet to be fully characterized. Here, we report...

    Authors: Nathan Kruer-Zerhusen, Markus Alahuhta, Vladimir V. Lunin, Michael E. Himmel, Yannick J. Bomble and David B. Wilson
    Citation: Biotechnology for Biofuels 2017 10:243
    Content type: Research Published on:

  40. Hydrothermal pretreatment using liquid hot water (LHW) is capable of substantially reducing the cell wall recalcitrance of lignocellulosic biomass. It enhances the saccharification of polysaccharides, particul...

    Authors: Mi Li, Shilin Cao, Xianzhi Meng, Michael Studer, Charles E. Wyman, Arthur J. Ragauskas and Yunqiao Pu
    Citation: Biotechnology for Biofuels 2017 10:237
    Content type: Research Published on:

  41. Efficient deconstruction and bioconversion of solids at high mass loadings is necessary to produce industrially relevant titers of biofuels from lignocellulosic biomass. To date, only a few studies have invest...

    Authors: Tobin J. Verbeke, Gabriela M. Garcia and James G. Elkins
    Citation: Biotechnology for Biofuels 2017 10:233
    Content type: Research Published on:

  42. Genetically engineered biofuel crops, such as switchgrass (Panicum virgatum L.), that produce their own cell wall-digesting cellulase enzymes would reduce costs of cellulosic biofuel production. To date, non-bioe...

    Authors: Jonathan D. Willis, Joshua N. Grant, Mitra Mazarei, Lindsey M. Kline, Caroline S. Rempe, A. Grace Collins, Geoffrey B. Turner, Stephen R. Decker, Robert W. Sykes, Mark F. Davis, Nicole Labbe, Juan L. Jurat-Fuentes and C. Neal Stewart Jr.
    Citation: Biotechnology for Biofuels 2017 10:230
    Content type: Research Published on:

  43. In the shadow of a burgeoning biomass-to-fuels industry, biological conversion of lignocellulose to fermentable sugars in a cost-effective manner is key to the success of second-generation and advanced biofuel...

    Authors: Michael E. Himmel, Charles A. Abbas, John O. Baker, Edward A. Bayer, Yannick J. Bomble, Roman Brunecky, Xiaowen Chen, Claus Felby, Tina Jeoh, Rajeev Kumar, Barry V. McCleary, Brett I. Pletschke, Melvin P. Tucker, Charles E. Wyman and Stephen R. Decker
    Citation: Biotechnology for Biofuels 2017 10:283
    Content type: Commentary Published on:

  44. Nitrogen deprivation and replenishment induces massive changes at the physiological and molecular level in the green alga Chlamydomonas reinhardtii, including reversible starch and lipid accumulation. Stress sign...

    Authors: Valentin Roustan, Shiva Bakhtiari, Pierre-Jean Roustan and Wolfram Weckwerth
    Citation: Biotechnology for Biofuels 2017 10:280
    Content type: Research Published on:

  45. The transition to a more environmentally friendly economy has prompted studies of modern biorefineries, including the utilization of low-value lignocellulose. The major challenge facing the widespread applicat...

    Authors: Yu-Si Yan, Shuai Zhao, Lu-Sheng Liao, Qi-Peng He, Ya-Ru Xiong, Long Wang, Cheng-Xi Li and Jia-Xun Feng
    Citation: Biotechnology for Biofuels 2017 10:279
    Content type: Research Published on:

  46. Trichoderma reesei is considered a candidate fungal enzyme producer for the economic saccharification of cellulosic biomass. However, performance of the saccharifying enzymes produced ...

    Authors: Nozomu Shibata, Mari Suetsugu, Hiroshi Kakeshita, Kazuaki Igarashi, Hiroshi Hagihara and Yasushi Takimura
    Citation: Biotechnology for Biofuels 2017 10:278
    Content type: Research Published on:

  47. A future bioeconomy relies on the efficient use of renewable resources for energy and material product supply. In this context, biorefineries have been developed and play a key role in converting lignocellulos...

    Authors: Julian Lange, Felix Müller, Kerstin Bernecker, Nicolaus Dahmen, Ralf Takors and Bastian Blombach
    Citation: Biotechnology for Biofuels 2017 10:277
    Content type: Research Published on:

  48. Current approaches for quantification of major energy-storage forms in microalgae, including starch, protein and lipids, generally require cell cultivation to collect biomass followed by tedious and time-consu...

    Authors: Yuehui He, Peng Zhang, Shi Huang, Tingting Wang, Yuetong Ji and Jian Xu
    Citation: Biotechnology for Biofuels 2017 10:275
    Content type: Methodology Published on:

  49. Synechococcus elongatus UTEX 2973 is the fastest growing cyanobacterium characterized to date. Its genome was found to be 99.8% identical to S. elongatus 7942 yet it grows twice as fas...

    Authors: Mary H. Abernathy, Jingjie Yu, Fangfang Ma, Michelle Liberton, Justin Ungerer, Whitney D. Hollinshead, Saratram Gopalakrishnan, Lian He, Costas D. Maranas, Himadri B. Pakrasi, Doug K. Allen and Yinjie J. Tang
    Citation: Biotechnology for Biofuels 2017 10:273
    Content type: Research Published on:

  50. Lignocellulosic biomass is an important resource for renewable production of biofuels and bioproducts. Enzymes that deconstruct this biomass are critical for the viability of biomass-based biofuel production p...

    Authors: Timo Schuerg, Jan-Philip Prahl, Raphael Gabriel, Simon Harth, Firehiwot Tachea, Chyi-Shin Chen, Matthew Miller, Fabrice Masson, Qian He, Sarah Brown, Mona Mirshiaghi, Ling Liang, Lauren M. Tom, Deepti Tanjore, Ning Sun, Todd R. Pray…
    Citation: Biotechnology for Biofuels 2017 10:271
    Content type: Research Published on:
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Biotechnology for Biofuels and Bioproducts

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