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  1. Content type: Research

    An efficient hydrolysis of lignocellulosic substrates to soluble sugars for biofuel production necessitates the interplay and synergistic interaction of multiple enzymes. An optimized enzyme mixture is crucial...

    Authors: Hélène Billard, Abdelaziz Faraj, Nicolas Lopes Ferreira, Sandra Menir and Senta Heiss-Blanquet

    Citation: Biotechnology for Biofuels 2012 5:9

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  2. Content type: Research

    Dilute acid pretreatment is a promising pretreatment technology for the biochemical production of ethanol from lignocellulosic biomass. During dilute acid pretreatment, xylan depolymerizes to form soluble xylo...

    Authors: Xiaowen Chen, Joseph Shekiro, Mary Ann Franden, Wei Wang, Min Zhang, Erik Kuhn, David K Johnson and Melvin P Tucker

    Citation: Biotechnology for Biofuels 2012 5:8

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  3. Content type: Research

    Wax ester synthases (WSs) can synthesize wax esters from alcohols and fatty acyl coenzyme A thioesters. The knowledge of the preferred substrates for each WS allows the use of yeast cells for the production of...

    Authors: Shuobo Shi, Juan Octavio Valle-Rodríguez, Sakda Khoomrung, Verena Siewers and Jens Nielsen

    Citation: Biotechnology for Biofuels 2012 5:7

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  4. Content type: Research

    Second generation hydrogen fermentation technologies using organic agricultural and forestry wastes are emerging. The efficient microbial fermentation of hexoses and pentoses resulting from the pretreatment of...

    Authors: Angela A Abreu, Dimitar Karakashev, Irini Angelidaki, Diana Z Sousa and M Madalena Alves

    Citation: Biotechnology for Biofuels 2012 5:6

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  5. Content type: Research

    There is currently considerable interest in developing renewable sources of energy. One strategy is the biological conversion of plant biomass to liquid transportation fuel. Several technical hurdles impinge u...

    Authors: Scott J Lee, Thomas A Warnick, Sivakumar Pattathil, Jesús G Alvelo-Maurosa, Michelle J Serapiglia, Heather McCormick, Virginia Brown, Naomi F Young, Danny J Schnell, Lawrence B Smart, Michael G Hahn, Jeffrey F Pedersen, Susan B Leschine and Samuel P Hazen

    Citation: Biotechnology for Biofuels 2012 5:5

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  6. Content type: Research

    Microbial lipids have drawn increasing attention in recent years as promising raw materials for biodiesel production, and the use of lignocellulosic hydrolysates as carbon sources seems to be a feasible strate...

    Authors: Chao Huang, Hong Wu, Zong-jun Liu, Jun Cai, Wen-yong Lou and Min-hua Zong

    Citation: Biotechnology for Biofuels 2012 5:4

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  7. Content type: Research

    Improving the hydrolytic performance of hemicellulases on lignocellulosic biomass is of considerable importance for second-generation biorefining. To address this problem, and also to gain greater understandin...

    Authors: Letian Song, Béatrice Siguier, Claire Dumon, Sophie Bozonnet and Michael J O'Donohue

    Citation: Biotechnology for Biofuels 2012 5:3

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  8. Content type: Research

    The model bacterium Clostridium cellulolyticum efficiently degrades crystalline cellulose and hemicellulose, using cellulosomes to degrade lignocellulosic biomass. Although it imports and ferments both pentose an...

    Authors: Yongchao Li, Timothy J Tschaplinski, Nancy L Engle, Choo Y Hamilton, Miguel Rodriguez Jr, James C Liao, Christopher W Schadt, Adam M Guss, Yunfeng Yang and David E Graham

    Citation: Biotechnology for Biofuels 2012 5:2

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  9. Content type: Research

    The ascomycete fungus, Trichoderma reesei (anamorph of Hypocrea jecorina), represents a biotechnological workhorse and is currently one of the most proficient cellulase producers. While strain improvement was tra...

    Authors: André Schuster, Kenneth S Bruno, James R Collett, Scott E Baker, Bernhard Seiboth, Christian P Kubicek and Monika Schmoll

    Citation: Biotechnology for Biofuels 2012 5:1

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  10. Content type: Methodology

    We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-ric...

    Authors: Hugo Pereira, Luísa Barreira, André Mozes, Cláudia Florindo, Cristina Polo, Catarina V Duarte, Luísa Custódio and João Varela

    Citation: Biotechnology for Biofuels 2011 4:61

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  11. Content type: Research

    Due to the complexity of lignocellulosic materials, a complete enzymatic hydrolysis into fermentable sugars requires a variety of cellulolytic and xylanolytic enzymes. Addition of xylanases has been shown to s...

    Authors: Junhua Zhang, Matti Siika-aho, Maija Tenkanen and Liisa Viikari

    Citation: Biotechnology for Biofuels 2011 4:60

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  12. Content type: Research

    Contamination of bacteria in large-scale yeast fermentations is a serious problem and a threat to the development of successful biofuel production plants. Huge research efforts have been spent in order to solv...

    Authors: Eva Albers, Emma Johansson, Carl Johan Franzén and Christer Larsson

    Citation: Biotechnology for Biofuels 2011 4:59

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  13. Content type: Research

    Large-scale production of effective cellulose hydrolytic enzymes is the key to the bioconversion of agricultural residues to ethanol. The goal of this study was to develop a rice plant as a bioreactor for the ...

    Authors: Hong Li Chou, Ziyu Dai, Chia Wen Hsieh and Maurice SB Ku

    Citation: Biotechnology for Biofuels 2011 4:58

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  14. Content type: Research

    The optimization of industrial bioethanol production will depend on the rational design and manipulation of industrial strains to improve their robustness against the many stress factors affecting their perfor...

    Authors: Francisco B Pereira, Pedro MR Guimarães, Daniel G Gomes, Nuno P Mira, Miguel C Teixeira, Isabel Sá-Correia and Lucília Domingues

    Citation: Biotechnology for Biofuels 2011 4:57

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  15. Content type: Research

    Solid acid catalyst was prepared from Kraft lignin by chemical activation with phosphoric acid, pyrolysis and sulfuric acid. This catalyst had high acid density as characterized by scanning electron microscope...

    Authors: Fei-ling Pua, Zhen Fang, Sarani Zakaria, Feng Guo and Chin-hua Chia

    Citation: Biotechnology for Biofuels 2011 4:56

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    The Erratum to this article has been published in Biotechnology for Biofuels 2012 5:66

  16. Content type: Research

    The recalcitrance of lignocellulosic materials is a major limitation for their conversion into fermentable sugars. Lignin depletion in new cultivars or transgenic plants has been identified as a way to diminis...

    Authors: Fernando Masarin, Daniela B Gurpilhares, David CF Baffa, Márcio HP Barbosa, Walter Carvalho, André Ferraz and Adriane MF Milagres

    Citation: Biotechnology for Biofuels 2011 4:55

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  17. Content type: Research

    In recent years, biorefining of lignocellulosic biomass to produce multi-products such as ethanol and other biomaterials has become a dynamic research area. Pretreatment technologies that fractionate sugarcane...

    Authors: Camila Alves Rezende, Marisa Aparecida de Lima, Priscila Maziero, Eduardo Ribeiro deAzevedo, Wanius Garcia and Igor Polikarpov

    Citation: Biotechnology for Biofuels 2011 4:54

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  18. Content type: Research

    Mixtures of prairie species (mixed prairie species; MPS) have been proposed to offer important advantages as a feedstock for sustainable production of fuels and chemicals. Therefore, understanding the performa...

    Authors: Jaclyn D DeMartini and Charles E Wyman

    Citation: Biotechnology for Biofuels 2011 4:52

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  19. Content type: Research

    The description of new hydrolytic enzymes is an important step in the development of techniques which use lignocellulosic materials as a starting point for fuel production. Sugarcane bagasse, which is subjecte...

    Authors: Severino A Lucena, Leile S Lima, Luís SA Cordeiro Jr, Celso Sant'Anna, Reginaldo Constantino, Patricia Azambuja, Wanderley de Souza, Eloi S Garcia and Fernando A Genta

    Citation: Biotechnology for Biofuels 2011 4:51

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  20. Content type: Research

    Lignocellulosic materials have been moved towards the forefront of the biofuel industry as a sustainable resource. However, saccharification and the production of bioproducts derived from plant cell wall bioma...

    Authors: João Paulo L Franco Cairo, Flávia C Leonardo, Thabata M Alvarez, Daniela A Ribeiro, Fernanda Büchli, Ana M Costa-Leonardo, Marcelo F Carazzolle, Fernando F Costa, Adriana F Paes Leme, Gonçalo AG Pereira and Fabio M Squina

    Citation: Biotechnology for Biofuels 2011 4:50

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  21. Content type: Research

    Softwoods are the dominant source of lignocellulosic biomass in the northern hemisphere, and have been investigated worldwide as a renewable substrate for cellulosic ethanol production. One challenge to using ...

    Authors: Gary M Hawkins and Joy Doran-Peterson

    Citation: Biotechnology for Biofuels 2011 4:49

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  22. Content type: Research

    Recently developed iron cocatalyst enhancement of dilute acid pretreatment of biomass is a promising approach for enhancing sugar release from recalcitrant lignocellulosic biomass. However, very little is know...

    Authors: Hui Wei, Bryon S Donohoe, Todd B Vinzant, Peter N Ciesielski, Wei Wang, Lynn M Gedvilas, Yining Zeng, David K Johnson, Shi-You Ding, Michael E Himmel and Melvin P Tucker

    Citation: Biotechnology for Biofuels 2011 4:48

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  23. Content type: Research

    Biodiesel is considered to be a promising future substitute for fossil fuels, and microalgae are one source of biodiesel. The ratios of lipid, carbohydrates and proteins are different in different microalgal s...

    Authors: Cheng Yuan, Junhan Liu, Yong Fan, Xiaohui Ren, Guangrong Hu and Fuli Li

    Citation: Biotechnology for Biofuels 2011 4:47

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  24. Content type: Research

    In the normal process of bioethanol production, biomass is transported to integrated large factories for degradation to sugar, fermentation, and recovery of ethanol by distillation. Biomass nutrient loss occur...

    Authors: Hiroko K Kitamoto, Mitsuo Horita, Yimin Cai, Yukiko Shinozaki and Keiji Sakaki

    Citation: Biotechnology for Biofuels 2011 4:46

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  25. Content type: Research

    Lignin is a highly abundant biopolymer synthesized by plants as a complex component of plant secondary cell walls. Efforts to utilize lignin-based bioproducts are needed.

    Authors: Venugopal Mendu, Anne E Harman-Ware, Mark Crocker, Jungho Jae, Jozsef Stork, Samuel Morton III, Andrew Placido, George Huber and Seth DeBolt

    Citation: Biotechnology for Biofuels 2011 4:43

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  26. Content type: Research

    The enzymatic production of biodiesel through alcoholysis of triglycerides has become more attractive because it shows potential in overcoming the drawbacks of chemical processes. In this study, we investigate...

    Authors: Koei Kawakami, Yasuhiro Oda and Ryo Takahashi

    Citation: Biotechnology for Biofuels 2011 4:42

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  27. Content type: Research

    In converting biomass to bioethanol, pretreatment is a key step intended to render cellulose more amenable and accessible to cellulase enzymes and thus increase glucose yields. In this study, four cellulose sa...

    Authors: Ashutosh Mittal, Rui Katahira, Michael E Himmel and David K Johnson

    Citation: Biotechnology for Biofuels 2011 4:41

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  28. Content type: Research

    Considering that the costs of cellulases and hemicellulases contribute substantially to the price of bioethanol, new studies aimed at understanding and improving cellulase efficiency and productivity are of pa...

    Authors: Wagner R de Souza, Paula F de Gouvea, Marcela Savoldi, Iran Malavazi, Luciano A de Souza Bernardes, Maria Helena S Goldman, Ronald P de Vries, Juliana V de Castro Oliveira and Gustavo H Goldman

    Citation: Biotechnology for Biofuels 2011 4:40

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  29. Content type: Research

    Hydrolysates of plant biomass used for the production of lignocellulosic biofuels typically contain sugar mixtures consisting mainly of D-glucose and D-xylose, and minor amounts of L-arabinose. The yeast Saccharo...

    Authors: Thorsten Subtil and Eckhard Boles

    Citation: Biotechnology for Biofuels 2011 4:38

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  30. Content type: Research

    Irpex lacteus, a versatile lignin-degrading fungus with various extracellular enzymes, has been widely used for biological pretreatment. However, most studies have focused on the change of substrate structure aft...

    Authors: Wanqing Du, Hongbo Yu, Lili Song, Ji Zhang, Changlong Weng, Fuying Ma and Xiaoyu Zhang

    Citation: Biotechnology for Biofuels 2011 4:37

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  31. Content type: Research

    We and other workers have shown that accessory enzymes, such as β-glucosidase, xylanase, and cellulase cofactors, such as GH61, can considerably enhance the hydrolysis effectiveness of cellulase cocktails when...

    Authors: Jinguang Hu, Valdeir Arantes and Jack N Saddler

    Citation: Biotechnology for Biofuels 2011 4:36

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  32. Content type: Research

    Ethanol production from paper sludge (PS) by simultaneous saccharification and fermentation (SSF) is considered to be the most appropriate way to process PS, as it contains negligible lignin. In this study, SS...

    Authors: Joni Prasetyo, Kazuya Naruse, Tatsuya Kato, Chuenchit Boonchird, Satoshi Harashima and Enoch Y Park

    Citation: Biotechnology for Biofuels 2011 4:35

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  33. Content type: Research

    Microalgae are a promising feedstock for biofuel and bioenergy production due to their high photosynthetic efficiencies, high growth rates and no need for external organic carbon supply. In this study, utiliza...

    Authors: Aino-Maija Lakaniemi, Christopher J Hulatt, David N Thomas, Olli H Tuovinen and Jaakko A Puhakka

    Citation: Biotechnology for Biofuels 2011 4:34

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  34. Content type: Research

    In order to generate biofuels, insoluble cellulosic substrates are pretreated andsubsequently hydrolyzed with cellulases. One way to pretreat cellulose in a safeand environmentally friendly manner is to apply,...

    Authors: Gernot Jäger, Michele Girfoglio, Florian Dollo, Roberto Rinaldi, Hans Bongard, Ulrich Commandeur, Rainer Fischer, Antje C Spiess and Jochen Büchs

    Citation: Biotechnology for Biofuels 2011 4:33

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  35. Content type: Research

    Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H2) producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The ...

    Authors: Mattias Ljunggren, Karin Willquist, Guido Zacchi and Ed WJ van Niel

    Citation: Biotechnology for Biofuels 2011 4:31

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  36. Content type: Research

    The main technological impediment to widespread utilization of lignocellulose for the production of fuels and chemicals is the lack of low-cost technologies to overcome its recalcitrance. Organisms that hydrol...

    Authors: Marja Ilmén, Riaan den Haan, Elena Brevnova, John McBride, Erin Wiswall, Allan Froehlich, Anu Koivula, Sanni P Voutilainen, Matti Siika-aho, Daniël C la Grange, Naomi Thorngren, Simon Ahlgren, Mark Mellon, Kristen Deleault, Vineet Rajgarhia, Willem H van Zyl…

    Citation: Biotechnology for Biofuels 2011 4:30

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  37. Content type: Research

    Cellulases and related hydrolytic enzymes represent a key cost factor for biochemical conversion of cellulosic biomass feedstocks to sugars for biofuels and chemicals production. The US Department of Energy (D...

    Authors: James D McMillan, Edward W Jennings, Ali Mohagheghi and Mildred Zuccarello

    Citation: Biotechnology for Biofuels 2011 4:29

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  38. Content type: Methodology

    Atmospheric pressure chemical ionisation mass spectrometry (APCI-MS) offers advantages as a rapid analytical technique for the quantification of three biomass degradation products (acetic acid, formic acid and...

    Authors: Scott M Davies, Rob S Linforth, Stuart J Wilkinson, Katherine A Smart and David J Cook

    Citation: Biotechnology for Biofuels 2011 4:28

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  39. Content type: Research

    The trichothecene mycotoxin deoxynivalenol (DON) may be concentrated in distillers dried grains with solubles (DDGS; a co-product of fuel ethanol fermentation) when grain containing DON is used to produce fuel...

    Authors: Piyum A Khatibi, Justin Montanti, Nhuan P Nghiem, Kevin B Hicks, Greg Berger, Wynse S Brooks, Carl A Griffey and David G Schmale III

    Citation: Biotechnology for Biofuels 2011 4:26

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  40. Content type: Research

    Biochemical conversion of lignocellulose hydrolysates remains challenging, largely because most microbial processes have markedly reduced efficiency in the presence of both hexoses and pentoses. Thus, identifi...

    Authors: Cuimin Hu, Siguo Wu, Qian Wang, Guojie Jin, Hongwei Shen and Zongbao K Zhao

    Citation: Biotechnology for Biofuels 2011 4:25

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  41. Content type: Research

    Neocallimastix patriciarum is one of the common anaerobic fungi in the digestive tracts of ruminants that can actively digest cellulosic materials, and its cellulases have great potential for hydrolyzing cellulos...

    Authors: Tzi-Yuan Wang, Hsin-Liang Chen, Mei-Yeh J Lu, Yo-Chia Chen, Huang-Mo Sung, Chi-Tang Mao, Hsing-Yi Cho, Huei-Mien Ke, Teh-Yang Hwa, Sz-Kai Ruan, Kuo-Yen Hung, Chih-Kuan Chen, Jeng-Yi Li, Yueh-Chin Wu, Yu-Hsiang Chen, Shao-Pei Chou…

    Citation: Biotechnology for Biofuels 2011 4:24

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  42. Content type: Research

    To efficiently deconstruct recalcitrant plant biomass to fermentable sugars in industrial processes, biocatalysts of higher performance and lower cost are required. The genetic diversity found in the metagenom...

    Authors: Luen-Luen Li, Safiyh Taghavi, Sean M McCorkle, Yian-Biao Zhang, Michael G Blewitt, Roman Brunecky, William S Adney, Michael E Himmel, Phillip Brumm, Colleen Drinkwater, David A Mead, Susannah G Tringe and Daniel van der Lelie

    Citation: Biotechnology for Biofuels 2011 4:23

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  43. Content type: Research

    As the supply of starch grain and sugar cane, currently the main feedstocks for bioethanol production, become limited, lignocelluloses will be sought as alternative materials for bioethanol production. Product...

    Authors: Yong Tang, Danqing Zhao, Carrasco Cristhian and Jianxin Jiang

    Citation: Biotechnology for Biofuels 2011 4:22

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  44. Content type: Research

    Isobutanol can be a better biofuel than ethanol due to its higher energy density and lower hygroscopicity. Furthermore, the branched-chain structure of isobutanol gives a higher octane number than the isomeric n-...

    Authors: Xiao Chen, Kristian F Nielsen, Irina Borodina, Morten C Kielland-Brandt and Kaisa Karhumaa

    Citation: Biotechnology for Biofuels 2011 4:21

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