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  1. Content type: Research

    Different mechanistic models have been used in the literature to describe the enzymatic hydrolysis of pretreated biomass. Although these different models have been applied to different substrates, most of thes...

    Authors: Russell F Brown, Frank K Agbogbo and Mark T Holtzapple

    Citation: Biotechnology for Biofuels 2010 3:6

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  2. Content type: Research

    The enzymatic hydrolysis of cellulose is still considered as one of the main limiting steps of the biological production of biofuels from lignocellulosic biomass. It is a complex multistep process, and various...

    Authors: Marie Chauve, Hugues Mathis, Delphine Huc, Dominique Casanave, Frédéric Monot and Nicolas Lopes Ferreira

    Citation: Biotechnology for Biofuels 2010 3:3

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  3. Content type: Research

    Biofuels offer a viable alternative to petroleum-based fuel. However, current methods are not sufficient and the technology required in order to use lignocellulosic biomass as a fermentation substrate faces se...

    Authors: Sandra A Allen, William Clark, J Michael McCaffery, Zhen Cai, Alison Lanctot, Patricia J Slininger, Z Lewis Liu and Steven W Gorsich

    Citation: Biotechnology for Biofuels 2010 3:2

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  4. Content type: Research

    When producing biofuels from dedicated feedstock, agronomic factors such as harvest time and location can impact the downstream production. Thus, this paper studies the effectiveness of ammonia fibre expansion...

    Authors: Bryan Bals, Chad Rogers, Mingjie Jin, Venkatesh Balan and Bruce Dale

    Citation: Biotechnology for Biofuels 2010 3:1

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  5. Content type: Research

    In this study, the dilute maleic acid pretreatment of wheat straw is optimized, using pretreatment time, temperature and maleic acid concentration as design variables. A central composite design was applied to...

    Authors: A Maarten J Kootstra, Hendrik H Beeftink, Elinor L Scott and Johan PM Sanders

    Citation: Biotechnology for Biofuels 2009 2:31

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  6. Content type: Research

    Pretreatment chemistry is of central importance due to its impacts on cellulosic biomass processing and biofuels conversion. Ammonia fiber expansion (AFEX) and dilute acid are two promising pretreatments using...

    Authors: Ming W Lau, Christa Gunawan and Bruce E Dale

    Citation: Biotechnology for Biofuels 2009 2:30

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  7. Content type: Research

    Corn stover composition changes considerably throughout the growing season and also varies between the various fractions of the plant. These differences can impact optimal pretreatment conditions, enzymatic di...

    Authors: Rebecca J Garlock, Shishir PS Chundawat, Venkatesh Balan and Bruce E Dale

    Citation: Biotechnology for Biofuels 2009 2:29

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  8. Content type: Research

    Screening new lignocellulosic biomass pretreatments and advanced enzyme systems at process relevant conditions is a key factor in the development of economically viable lignocellulosic ethanol. Shake flasks, t...

    Authors: Christine M Roche, Clare J Dibble and Jonathan J Stickel

    Citation: Biotechnology for Biofuels 2009 2:28

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  9. Content type: Research

    Termite lignocellulose digestion is achieved through a collaboration of host plus prokaryotic and eukaryotic symbionts. In the present work, we took a combined host and symbiont metatranscriptomic approach for...

    Authors: Aurélien Tartar, Marsha M Wheeler, Xuguo Zhou, Monique R Coy, Drion G Boucias and Michael E Scharf

    Citation: Biotechnology for Biofuels 2009 2:25

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  10. Content type: Research

    Bioethanol isolated from lignocellulosic biomass represents one of the most promising renewable and carbon neutral alternative liquid fuel sources. Enzymatic saccharification using cellulase has proven to be a...

    Authors: Tatsuya Fujii, Xu Fang, Hiroyuki Inoue, Katsuji Murakami and Shigeki Sawayama

    Citation: Biotechnology for Biofuels 2009 2:24

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  11. Content type: Commentary

    Mary E H Mandels, who spearheaded the US Army's national bioconversion studies for four decades and was an early proponent of conversion of waste biomass to readily bioconvertible sugars for the production of ...

    Authors: Fred Allen, Raymond Andreotti, Douglas E Eveleigh and John Nystrom

    Citation: Biotechnology for Biofuels 2009 2:22

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  12. Content type: Commentary

    This article sets forth a simple cellulase assay procedure. Cellulose is variable in nature, insoluble and resistant to enzymatic attack. As a result there have been a bevy of bewildering cellulase assays publ...

    Authors: Douglas E Eveleigh, Mary Mandels, Raymond Andreotti and Charles Roche

    Citation: Biotechnology for Biofuels 2009 2:21

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  13. Content type: Commentary

    This article, 'Reflections on the United States Military 1941-1987' written by my grandmother, Mary Mandels, illustrates her passion for life. Her outreach article was considered most appropriate for publicati...

    Authors: Mary Mandels and Douglas E Eveleigh

    Citation: Biotechnology for Biofuels 2009 2:20

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  14. Content type: Review

    Hypocrea jecorina (= Trichoderma reesei) is the main industrial source of cellulases and hemicellulases used to depolymerise plant biomass to simple sugars that are converted to chemical intermediates and biofuel...

    Authors: Christian P Kubicek, Marianna Mikus, André Schuster, Monika Schmoll and Bernhard Seiboth

    Citation: Biotechnology for Biofuels 2009 2:19

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  15. Content type: Research

    Two economic factors make watermelon worthy of consideration as a feedstock for ethanol biofuel production. First, about 20% of each annual watermelon crop is left in the field because of surface blemishes or ...

    Authors: Wayne W Fish, Benny D Bruton and Vincent M Russo

    Citation: Biotechnology for Biofuels 2009 2:18

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  16. Content type: Research

    Drying is currently the most frequently used conservation method for cereal grain, which in temperate climates consumes a major part of process energy. Airtight storage of moist feed grain using the biocontrol...

    Authors: Volkmar Passoth, Anna Eriksson, Mats Sandgren, Jerry Ståhlberg, Kathleen Piens and Johan Schnürer

    Citation: Biotechnology for Biofuels 2009 2:16

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  17. Content type: Research

    Improvement of the process of cellulase production and development of more efficient lignocellulose-degrading enzymes are necessary in order to reduce the cost of enzymes required in the biomass-to-bioethanol ...

    Authors: Krisztina Kovacs, Stefano Macrelli, George Szakacs and Guido Zacchi

    Citation: Biotechnology for Biofuels 2009 2:14

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  18. Content type: Research

    This work explores the potential contribution of bioenergy technologies to 60% and 80% carbon reductions in the UK energy system by 2050, by outlining the potential for accelerated technological development of...

    Authors: Donna Clarke, Sophie Jablonski, Brighid Moran, Gabrial Anandarajah and Gail Taylor

    Citation: Biotechnology for Biofuels 2009 2:13

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  19. Content type: Research

    The production of hydrogen from biomass by fermentation is one of the routes that can contribute to a future sustainable hydrogen economy. Lignocellulosic biomass is an attractive feedstock because of its abun...

    Authors: Truus de Vrije, Robert R Bakker, Miriam AW Budde, Man H Lai, Astrid E Mars and Pieternel AM Claassen

    Citation: Biotechnology for Biofuels 2009 2:12

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  20. Content type: Review

    Throughout immeasurable time, microorganisms evolved and accumulated remarkable physiological and functional heterogeneity, and now constitute the major reserve for genetic diversity on earth. Using metagenomi...

    Authors: Luen-Luen Li, Sean R McCorkle, Sebastien Monchy, Safiyh Taghavi and Daniel van der Lelie

    Citation: Biotechnology for Biofuels 2009 2:10

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  21. Content type: Research

    Xylose reductase (XR) and xylitol dehydrogenase (XDH) from Pichia stipitis are the two enzymes most commonly used in recombinant Saccharomyces cerevisiae strains engineered for xylose utilization. The availabilit...

    Authors: Oskar Bengtsson, Bärbel Hahn-Hägerdal and Marie F Gorwa-Grauslund

    Citation: Biotechnology for Biofuels 2009 2:9

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  22. Content type: Research

    Simultaneous saccharification and fermentation (SSF) is a promising process option for ethanol production from lignocellulosic materials. However, both the overall ethanol yield and the final ethanol concentra...

    Authors: Magnus Bertilsson, Kim Olofsson and Gunnar Lidén

    Citation: Biotechnology for Biofuels 2009 2:8

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  23. Content type: Research

    Microbial fuel cells (MFC) and microbial electrolysis cells are electrical devices that treat water using microorganisms and convert soluble organic matter into electricity and hydrogen, respectively. Emerging...

    Authors: Abhijeet P Borole, Jonathan R Mielenz, Tatiana A Vishnivetskaya and Choo Y Hamilton

    Citation: Biotechnology for Biofuels 2009 2:7

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  24. Content type: Research

    Two-step dilute acid hydrolysis of softwood, either as a stand-alone process or as pretreatment before enzymatic hydrolysis, is considered to result in higher sugar yields than one-step acid hydrolysis. Howeve...

    Authors: Sanam Monavari, Mats Galbe and Guido Zacchi

    Citation: Biotechnology for Biofuels 2009 2:6

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  25. Content type: Research

    Improvement of biofeedstock quality for cellulosic ethanol production will be facilitated by inexpensive and rapid methods of evaluation, such as those already employed in the field of ruminant nutrition. Our ...

    Authors: Aaron J Lorenz, Rob P Anex, Asli Isci, James G Coors, Natalia de Leon and Paul J Weimer

    Citation: Biotechnology for Biofuels 2009 2:5

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  26. Content type: Research

    Enzymatic corn wet milling (E-milling) is a process derived from conventional wet milling for the recovery and purification of starch and co-products using proteases to eliminate the need for sulfites and decr...

    Authors: Edna C Ramírez, David B Johnston, Andrew J McAloon and Vijay Singh

    Citation: Biotechnology for Biofuels 2009 2:2

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  27. Content type: Research

    Transesterification of Jatropha oil was carried out in t-butanol solvent using immobilized lipase from Enterobacter aerogenes. The presence of t-butanol significantly reduced the negative effects caused by both m...

    Authors: Annapurna Kumari, Paramita Mahapatra, Vijay Kumar Garlapati and Rintu Banerjee

    Citation: Biotechnology for Biofuels 2009 2:1

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  28. Content type: Research

    Due to its capacity to produce large amounts of cellulases, Trichoderma reesei is increasingly been researched in various fields of white biotechnology, especially in biofuel production from lignocellulosic bioma...

    Authors: Isabelle Herpoël-Gimbert, Antoine Margeot, Alain Dolla, Gwénaël Jan, Daniel Mollé, Sabrina Lignon, Hughes Mathis, Jean-Claude Sigoillot, Frédéric Monot and Marcel Asther

    Citation: Biotechnology for Biofuels 2008 1:18

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  29. Content type: Research

    The availability and low cost of lignocellulosic biomass has caused tremendous interest in the bioconversion of this feedstock into liquid fuels. One measure of the economic viability of the bioconversion proc...

    Authors: Lisa J Haney, James G Coors, Aaron J Lorenz, DRaj Raman, Robert P Anex and MPaul Scott

    Citation: Biotechnology for Biofuels 2008 1:17

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  30. Content type: Research

    Ethanolic fermentation of lignocellulosic biomass is a sustainable option for the production of bioethanol. This process would greatly benefit from recombinant Saccharomyces cerevisiae strains also able to fermen...

    Authors: Maurizio Bettiga, Bärbel Hahn-Hägerdal and Marie F Gorwa-Grauslund

    Citation: Biotechnology for Biofuels 2008 1:16

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  31. Content type: Research

    Brachypodium distachyon constitutes an excellent model species for grasses. It is a small, easily propagated, temperate grass with a rapid life cycle and a small genome. It is a self-fertile plant that can be tra...

    Authors: Leonardo D Gomez, Jennifer K Bristow, Emily R Statham and Simon J McQueen-Mason

    Citation: Biotechnology for Biofuels 2008 1:15

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  32. Content type: Research

    The limited availability of fossil fuel sources, worldwide rising energy demands and anticipated climate changes attributed to an increase of greenhouse gasses are important driving forces for finding alternat...

    Authors: Ronald HW Maas, Robert R Bakker, Arjen R Boersma, Iemke Bisschops, Jan R Pels, Ed de Jong, Ruud A Weusthuis and Hans Reith

    Citation: Biotechnology for Biofuels 2008 1:14

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  33. Content type: Methodology

    Lignocellulosic bioethanol technologies exhibit significant capacity for performance improvement across the supply chain through the development of high-yielding energy crops, integrated pretreatment, hydrolys...

    Authors: Alex J Dunnett, Claire S Adjiman and Nilay Shah

    Citation: Biotechnology for Biofuels 2008 1:13

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  34. Content type: Research

    Pichia stipitis xylose reductase (Ps-XR) has been used to design Saccharomyces cerevisiae strains that are able to ferment xylose. One example is the industrial S. cerevisiae xylose-consuming strain TMB3400, whic...

    Authors: João RM Almeida, Tobias Modig, Anja Röder, Gunnar Lidén and Marie-F Gorwa-Grauslund

    Citation: Biotechnology for Biofuels 2008 1:12

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  35. Content type: Methodology

    Engineering microorganisms to improve metabolite flux requires detailed knowledge of the concentrations and flux rates of metabolites and metabolic intermediates in vivo. Fluorescence resonance energy transfer se...

    Authors: Thijs Kaper, Ida Lager, Loren L Looger, Diane Chermak and Wolf B Frommer

    Citation: Biotechnology for Biofuels 2008 1:11

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  36. Content type: Research

    The cellulase producing ascomycete, Trichoderma reesei (Hypocrea jecorina), is known to secrete a range of enzymes important for ethanol production from lignocellulosic biomass. It is also widely used for the com...

    Authors: Tina Jeoh, William Michener, Michael E Himmel, Stephen R Decker and William S Adney

    Citation: Biotechnology for Biofuels 2008 1:10

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  37. Content type: Research

    A metabolic regulation study was performed, based upon measurements of enzymatic activities, fermentation performance, and RT-PCR analysis of pathways related to central carbon metabolism, in an ethanologenic Esc...

    Authors: Montserrat Orencio-Trejo, Noemí Flores, Adelfo Escalante, Georgina Hernández-Chávez, Francisco Bolívar, Guillermo Gosset and Alfredo Martinez

    Citation: Biotechnology for Biofuels 2008 1:8

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  38. Content type: Review

    Ethanol is a biofuel that is used as a replacement for approximately 3% of the fossil-based gasoline consumed in the world today. Most of this biofuel is produced from sugarcane in Brazil and corn in the Unite...

    Authors: José Goldemberg

    Citation: Biotechnology for Biofuels 2008 1:6

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