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  1. In an effort to find economical, carbon-neutral transportation fuels, biomass feedstock compositional analysis methods are used to monitor, compare, and improve biofuel conversion processes. These methods are ...

    Authors: David W. Templeton, Justin B. Sluiter, Amie Sluiter, Courtney Payne, David P. Crocker, Ling Tao and Ed. Wolfrum
    Citation: Biotechnology for Biofuels 2016 9:223
  2. The quest for sustainable production of renewable and cheap biofuels has triggered an intensive search for domestication of the next generation of bioenergy crops. Aquatic plants which can rapidly colonize wet...

    Authors: Ana F. Miranda, Bijoy Biswas, Narasimhan Ramkumar, Rawel Singh, Jitendra Kumar, Anton James, Felicity Roddick, Banwari Lal, Sanjukta Subudhi, Thallada Bhaskar and Aidyn Mouradov
    Citation: Biotechnology for Biofuels 2016 9:221
  3. The design of photobioreactor (PBR) for outdoor mass cultivation of microalgae determines the distribution of solar irradiance among cells in the culture, mode of agitation, mass transfer efficacy, and energy ...

    Authors: Jim Junhui Huang, Gagarin Bunjamin, Edwin Sianguan Teo, Deric Boonhuat Ng and Yuan Kun Lee
    Citation: Biotechnology for Biofuels 2016 9:218
  4. Starch is a very abundant and renewable carbohydrate and is an important feedstock for industrial applications. The conventional starch liquefaction and saccharification processes are energy-intensive, complic...

    Authors: Qiang-Sheng Xu, Yu-Si Yan and Jia-Xun Feng
    Citation: Biotechnology for Biofuels 2016 9:216
  5. Microbial synthesis of oleochemicals derived from native fatty acid (FA) metabolism has presented significant advances in recent years. Even so, native FA biosynthetic pathways often provide a narrow variety o...

    Authors: Simón Menendez-Bravo, Julia Roulet, Martín Sabatini, Santiago Comba, Robert Dunn, Hugo Gramajo and Ana Arabolaza
    Citation: Biotechnology for Biofuels 2016 9:215
  6. Limonene, a monocyclic monoterpene, is known for its using as an important precursor of many flavoring, pharmaceutical, and biodiesel products. Currently, d-limonene has been produced via fractionation from es...

    Authors: Xuan Cao, Yu-Bei Lv, Jun Chen, Tadayuki Imanaka, Liu-Jing Wei and Qiang Hua
    Citation: Biotechnology for Biofuels 2016 9:214
  7. Pretreatment is a critical step in the biochemical conversion of lignocellulosic biomass to fuels and chemicals. Due to the complexity of the physicochemical transformations involved, predictively scaling up t...

    Authors: James J. Lischeske, Nathan C. Crawford, Erik Kuhn, Nicholas J. Nagle, Daniel J. Schell, Melvin P. Tucker, James D. McMillan and Edward J. Wolfrum
    Citation: Biotechnology for Biofuels 2016 9:213
  8. Glycolysis breakdowns glucose into essential building blocks and ATP/NAD(P)H for the cell, occupying a central role in its growth and bio-production. Among glycolytic pathways, the Entner Doudoroff pathway (ED...

    Authors: Whitney D. Hollinshead, Sarah Rodriguez, Hector Garcia Martin, George Wang, Edward E. K. Baidoo, Kenneth L. Sale, Jay D. Keasling, Aindrila Mukhopadhyay and Yinjie J. Tang
    Citation: Biotechnology for Biofuels 2016 9:212
  9. Lignocellulolytic bacteria have revealed to be a promising source for biofuel production, yet the underlying mechanisms are still worth exploring. Our previous study inferred that the highly efficient lignocel...

    Authors: Jiangshan Ma, Keke Zhang, Mei Huang, Stanton B. Hector, Bin Liu, Chunyi Tong, Qian Liu, Jiarui Zeng, Yan Gao, Ting Xu, Ying Liu, Xuanming Liu and Yonghua Zhu
    Citation: Biotechnology for Biofuels 2016 9:211
  10. Due to the finite nature of global oil resources we are now faced with the challenge of finding renewable resources to produce fuels and chemicals in the future. Lactic acid has great potential as a precursor ...

    Authors: Martinus J. A. Daas, Antonius H. P. van de Weijer, Willem M. de Vos, John van der Oost and Richard van Kranenburg
    Citation: Biotechnology for Biofuels 2016 9:210
  11. 3-hydroxypropionic acid (3-HP) is an important platform chemical with a wide range of applications. In our previous study, the biosynthetic pathway of 3-HP was constructed and optimized in cyanobacterium Synechoc...

    Authors: Yunpeng Wang, Lei Chen and Weiwen Zhang
    Citation: Biotechnology for Biofuels 2016 9:209
  12. Microbial production of oleochemicals has been actively studied in the last decade. Free fatty acids (FFAs) could be converted into a variety of molecules such as industrial products, consumer products, and fu...

    Authors: Kwang Soo Shin, Sangwoo Kim and Sung Kuk Lee
    Citation: Biotechnology for Biofuels 2016 9:208
  13. To reduce the cost of the enzymes for the hydrolysis of lignocellulosic biomass, two main strategies have been followed: one, the reduction of enzyme dosing by the use of more efficient and stable enzymatic co...

    Authors: María Ángeles Bermúdez Alcántara, Justyna Dobruchowska, Parastoo Azadi, Bruno Díez García, Fernando P. Molina-Heredia and Francisco Manuel Reyes-Sosa
    Citation: Biotechnology for Biofuels 2016 9:207
  14. The conversion of lignocellulosic biomass to biofuels (second-generation biofuel production) is an environmentally friendlier alternative to petroleum-based energy sources. Enzymatic deconstruction of lignocel...

    Authors: Thaila Fernanda dos Reis, Pollyne Borborema Almeida de Lima, Nádia Skorupa Parachin, Fabiana Bombonato Mingossi, Juliana Velasco de Castro Oliveira, Laure Nicolas Annick Ries and Gustavo Henrique Goldman
    Citation: Biotechnology for Biofuels 2016 9:204
  15. The filamentous fungus Penicillium oxalicum is a potential alternative to Trichoderma reesei for industrial production of a complete cellulolytic enzyme system for a bio-refinery. Comparative omics approaches can...

    Authors: Shuai Zhao, Yu-Si Yan, Qi-Peng He, Lin Yang, Xin Yin, Cheng-Xi Li, Li-Chun Mao, Lu-Sheng Liao, Jin-Qun Huang, Shang-Bo Xie, Qing-Dong Nong, Zheng Zhang, Lei Jing, Ya-Ru Xiong, Cheng-Jie Duan, Jun-Liang Liu…
    Citation: Biotechnology for Biofuels 2016 9:203
  16. Metabolic engineering of cyanobacteria has enabled photosynthetic conversion of CO2 to value-added chemicals as bio-solar cell factories. However, the production levels of isoprenoids in engineered cyanobacteria ...

    Authors: Sun Young Choi, Hyun Jeong Lee, Jaeyeon Choi, Jiye Kim, Sang Jun Sim, Youngsoon Um, Yunje Kim, Taek Soon Lee, Jay D. Keasling and Han Min Woo
    Citation: Biotechnology for Biofuels 2016 9:202
  17. Glycolic acid (GA) is a two-carbon hydroxyacid with applications in the cosmetic, textile, and medical industry. Microbial GA production from all sugars can be achieved by engineering the natural glyoxylate sh...

    Authors: Ceren Alkim, Debora Trichez, Yvan Cam, Lucie Spina, Jean Marie François and Thomas Walther
    Citation: Biotechnology for Biofuels 2016 9:201
  18. Lignocellulosic biomass continues to be investigated as a viable source for bioethanol production. However, the pretreatment process generates inhibitory compounds that impair the growth and fermentation perfo...

    Authors: Olivia A. Thompson, Gary M. Hawkins, Steven W. Gorsich and Joy Doran-Peterson
    Citation: Biotechnology for Biofuels 2016 9:200
  19. Random mutagenesis is a powerful technique to obtain mutant proteins with different properties from the wild-type molecule. Error-prone PCR is often employed for random mutagenesis in bacterial protein express...

    Authors: Mikako Tachioka, Naohisa Sugimoto, Akihiko Nakamura, Naoki Sunagawa, Takuya Ishida, Taku Uchiyama, Kiyohiko Igarashi and Masahiro Samejima
    Citation: Biotechnology for Biofuels 2016 9:199
  20. Despite claims as key enzymes in enzymatic delignification, very scarce information on the reaction rates between the ligninolytic versatile peroxidase (VP) and lignin peroxidase (LiP) and the lignin polymer i...

    Authors: Verónica Sáez-Jiménez, Jorge Rencoret, Miguel Angel Rodríguez-Carvajal, Ana Gutiérrez, Francisco Javier Ruiz-Dueñas and Angel T. Martínez
    Citation: Biotechnology for Biofuels 2016 9:198
  21. Fermentable sugars are important intermediates in the biological conversion of biomass. Hemicellulose and amorphous cellulose are easily hydrolyzed to fermentable sugars in dilute acid, whereas crystalline cel...

    Authors: Liqun Jiang, Nannan Wu, Anqing Zheng, Zengli Zhao, Fang He and Haibin Li
    Citation: Biotechnology for Biofuels 2016 9:196
  22. Second generation (2G) bioenergy from lignocellulosic feedstocks has the potential to develop as a sustainable source of renewable energy; however, significant hurdles still remain for large-scale commercialis...

    Authors: Mike Robert Allwright, Adrienne Payne, Giovanni Emiliani, Suzanne Milner, Maud Viger, Franchesca Rouse, Joost J. B. Keurentjes, Aurélie Bérard, Henning Wildhagen, Patricia Faivre-Rampant, Andrea Polle, Michele Morgante and Gail Taylor
    Citation: Biotechnology for Biofuels 2016 9:195
  23. Clostridium thermocellum is a gram-positive thermophile that can directly convert lignocellulosic material into biofuels. The metabolism of C. thermocellum contains many branches and r...

    Authors: R. Adam Thompson, Sanjeev Dahal, Sergio Garcia, Intawat Nookaew and Cong T. Trinh
    Citation: Biotechnology for Biofuels 2016 9:194
  24. Rice cultivation produces two waste streams, straw and husk, which could be exploited more effectively. Chemical pretreatment studies using rice residues have largely focussed on straw exploitation alone, and ...

    Authors: Ian P. Wood, Huong-Giang Cao, Long Tran, Nicola Cook, Peter Ryden, David R. Wilson, Graham K. Moates, Samuel R. A. Collins, Adam Elliston and Keith W. Waldron
    Citation: Biotechnology for Biofuels 2016 9:193
  25. The white-rot Agaricomycetes species Phlebia radiata is an efficient wood-decaying fungus degrading all wood components, including cellulose, hemicellulose, and lignin. We cultivated P. radiata in solid state cul...

    Authors: Jaana Kuuskeri, Mari Häkkinen, Pia Laine, Olli-Pekka Smolander, Fitsum Tamene, Sini Miettinen, Paula Nousiainen, Marianna Kemell, Petri Auvinen and Taina Lundell
    Citation: Biotechnology for Biofuels 2016 9:192
  26. Replacing fossil fuel with renewable sources such as lignocellulosic biomass is currently a promising alternative for obtaining biofuel and for fighting against the consequences of climate change. However, the...

    Authors: Sara Gómez, Asia M. Payne, Martin Savko, Gavin C. Fox, William E. Shepard, Francisco J. Fernandez and M. Cristina Vega
    Citation: Biotechnology for Biofuels 2016 9:191
  27. Elephant grass (Pennisetum purpureum Schumach) and Hybrid Pennisetum (Pennisetum americanum × P. purpureum Schumach) are tall, fast-growing perennial C4 bunchgrasses that have been in recent developed as the most...

    Authors: Xia Li, Xiaoyan Geng, Rongrong Xie, Lei Fu, Jianxiong Jiang, Lu Gao and Jianzhong Sun
    Citation: Biotechnology for Biofuels 2016 9:190
  28. To develop pathways for advanced biofuel production, and to understand the impact of host metabolism and environmental conditions on heterologous pathway engineering for economic advanced biofuels production f...

    Authors: Shihui Yang, Ali Mohagheghi, Mary Ann Franden, Yat-Chen Chou, Xiaowen Chen, Nancy Dowe, Michael E. Himmel and Min Zhang
    Citation: Biotechnology for Biofuels 2016 9:189
  29. Mannans represent the largest hemicellulosic fraction in softwoods and also serve as carbohydrate stores in various plants. However, the utilization of mannans as sustainable resources has been less advanced i...

    Authors: Jun Ishii, Fumiyoshi Okazaki, Apridah Cameliawati Djohan, Kiyotaka Y. Hara, Nanami Asai-Nakashima, Hiroshi Teramura, Ade Andriani, Masahiro Tominaga, Satoshi Wakai, Prihardi Kahar, Yopi, Bambang Prasetya, Chiaki Ogino and Akihiko Kondo
    Citation: Biotechnology for Biofuels 2016 9:188
  30. Starch is the second most abundant plant-derived biomass and a major feedstock in non-food industrial applications and first generation biofuel production. In contrast to lignocellulose, detailed insight into ...

    Authors: Laura Nekiunaite, Magnus Ø. Arntzen, Birte Svensson, Gustav Vaaje-Kolstad and Maher Abou Hachem
    Citation: Biotechnology for Biofuels 2016 9:187
  31. Lytic polysaccharide monooxgygenases (LPMOs) are known to boost the hydrolytic breakdown of lignocellulosic biomass, especially cellulose, due to their oxidative mechanism. For their activity, LPMOs require an...

    Authors: Matthias Frommhagen, Martijn J. Koetsier, Adrie H. Westphal, Jaap Visser, Sandra W. A. Hinz, Jean-Paul Vincken, Willem J. H. van Berkel, Mirjam A. Kabel and Harry Gruppen
    Citation: Biotechnology for Biofuels 2016 9:186
  32. Aldehyde-deformylating oxygenase (ADO) is an important enzyme involved in the biosynthetic pathway of fatty alk(a/e)nes in cyanobacteria. However, ADO exhibits quite low chain-length specificity with respect t...

    Authors: Luyao Bao, Jian-Jun Li, Chenjun Jia, Mei Li and Xuefeng Lu
    Citation: Biotechnology for Biofuels 2016 9:185
  33. Microalgae biofuel has become the most promising renewable energy over the past few years. But limitations still exist because of its high cost. Although, efforts have been made in enhancement of lipid product...

    Authors: Bin Jia, Yanzi Song, Min Wu, Baicheng Lin, Kang Xiao, Zhangli Hu and Ying Huang
    Citation: Biotechnology for Biofuels 2016 9:184
  34. Despite extensive research in the last decades, microalgae are still only economically feasible for high valued markets. Strain improvement is a strategy to increase productivities, hence reducing costs. In th...

    Authors: Iago Teles Dominguez Cabanelas, Mathijs van der Zwart, Dorinde M. M. Kleinegris, René H. Wijffels and Maria J. Barbosa
    Citation: Biotechnology for Biofuels 2016 9:183
  35. Hydrogen photo-production in green algae, catalyzed by the enzyme [FeFe]-hydrogenase (HydA), is considered a promising source of renewable clean energy. Yet, a significant increase in hydrogen production effic...

    Authors: Haviva Eilenberg, Iddo Weiner, Oren Ben-Zvi, Carmel Pundak, Abigail Marmari, Oded Liran, Matt S. Wecker, Yuval Milrad and Iftach Yacoby
    Citation: Biotechnology for Biofuels 2016 9:182
  36. Ultrafine grinding is an environmentally friendly pretreatment that can alter the degree of polymerization, the porosity and the specific surface area of lignocellulosic biomass and can, thus, enhance cellulos...

    Authors: Haiyan Zhang, Longjian Chen, Minsheng Lu, Junbao Li and Lujia Han
    Citation: Biotechnology for Biofuels 2016 9:181
  37. Increasing interest of non-conventional yeasts has been observed for many years due to their biochemical characteristics and potential applications. Well-studied, oleaginous yeast Y. lipolytica is an attractive h...

    Authors: Aleksandra M. Mirończuk, Dorota A. Rzechonek, Anna Biegalska, Magdalena Rakicka and Adam Dobrowolski
    Citation: Biotechnology for Biofuels 2016 9:180
  38. Integrating waste management with fuels and chemical production is considered to address the food waste problem and oil crisis. Approximately, 600 million tonnes crude glycerol is produced from the biodiesel i...

    Authors: Cuijuan Gao, Xiaofeng Yang, Huaimin Wang, Cristina Perez Rivero, Chong Li, Zhiyong Cui, Qingsheng Qi and Carol Sze Ki Lin
    Citation: Biotechnology for Biofuels 2016 9:179
  39. Through binding to cellulose, expansin-like proteins are thought to loosen the structural order of crystalline surface material, thus making it more accessible for degradation by hydrolytic enzymes. Swollenin ...

    Authors: Manuel Eibinger, Karin Sigl, Jürgen Sattelkow, Thomas Ganner, Jonas Ramoni, Bernhard Seiboth, Harald Plank and Bernd Nidetzky
    Citation: Biotechnology for Biofuels 2016 9:178
  40. Solid acid catalyzed inexpensive ionic liquid (IL) pretreatment is promising because of its effectiveness at decreasing biomass recalcitrance to subsequent enzymatic hydrolysis or in situ hydrolysis of carbohy...

    Authors: Tingting You, Lupeng Shao, Ruizhen Wang, Liming Zhang and Feng Xu
    Citation: Biotechnology for Biofuels 2016 9:177
  41. The ability to deconstruct plant biomass without conventional pretreatment has made members of the genus Caldicellulosiruptor the target of investigation for the consolidated processing of lignocellulosic biomass...

    Authors: Sun-Ki Kim, Daehwan Chung, Michael E. Himmel, Yannick J. Bomble and Janet Westpheling
    Citation: Biotechnology for Biofuels 2016 9:176
  42. Glycerol, a byproduct of biodiesel, has become a readily available and inexpensive carbon source for the production of high-value products. However, the main drawback of glycerol utilization is the low consump...

    Authors: Ruilian Yao, Dewang Xiong, Hongbo Hu, Masataka Wakayama, Wenjuan Yu, Xuehong Zhang and Kazuyuki Shimizu
    Citation: Biotechnology for Biofuels 2016 9:175
  43. In general, biofuel production involves biomass pretreatment and enzymatic saccharification, followed by the subsequent sugar conversion to biofuel via fermentation. The crucial step in the production of biofu...

    Authors: Chia Sing Chan, Lee Li Sin, Kok-Gan Chan, Mohd Shahir Shamsir, Fazilah Abd Manan, Rajesh Kumar Sani and Kian Mau Goh
    Citation: Biotechnology for Biofuels 2016 9:174
  44. Acetic acid, released during hydrolysis of lignocellulosic feedstocks for second generation bioethanol production, inhibits yeast growth and alcoholic fermentation. Yeast biomass generated in a propagation ste...

    Authors: Daniel González-Ramos, Arthur R. Gorter de Vries, Sietske S. Grijseels, Margo C. van Berkum, Steve Swinnen, Marcel van den Broek, Elke Nevoigt, Jean-Marc G. Daran, Jack T. Pronk and Antonius J. A. van Maris
    Citation: Biotechnology for Biofuels 2016 9:173
  45. Lignocellulosic biomass is one of earth’s most abundant resources, and it has great potential for biofuel production because it is renewable and has carbon-neutral characteristics. Lignocellulose is mainly com...

    Authors: Tao Sheng, Lei Zhao, Ling-Fang Gao, Wen-Zong Liu, Min-Hua Cui, Ze-Chong Guo, Xiao-Dan Ma, Shih-Hsin Ho and Ai-Jie Wang
    Citation: Biotechnology for Biofuels 2016 9:172
  46. One of the most promising technologies to sustainably produce energy and to mitigate greenhouse gas emissions from combustion of fossil energy carriers is the anaerobic digestion and biomethanation of organic ...

    Authors: Irena Maus, Daniela E. Koeck, Katharina G. Cibis, Sarah Hahnke, Yong S. Kim, Thomas Langer, Jana Kreubel, Marcel Erhard, Andreas Bremges, Sandra Off, Yvonne Stolze, Sebastian Jaenicke, Alexander Goesmann, Alexander Sczyrba, Paul Scherer, Helmut König…
    Citation: Biotechnology for Biofuels 2016 9:171