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  1. The recalcitrance of lignocellulosic materials is a major limitation for their conversion into fermentable sugars. Lignin depletion in new cultivars or transgenic plants has been identified as a way to diminis...

    Authors: Fernando Masarin, Daniela B Gurpilhares, David CF Baffa, Márcio HP Barbosa, Walter Carvalho, André Ferraz and Adriane MF Milagres

    Citation: Biotechnology for Biofuels 2011 4:55

    Content type: Research

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  2. In recent years, biorefining of lignocellulosic biomass to produce multi-products such as ethanol and other biomaterials has become a dynamic research area. Pretreatment technologies that fractionate sugarcane...

    Authors: Camila Alves Rezende, Marisa Aparecida de Lima, Priscila Maziero, Eduardo Ribeiro deAzevedo, Wanius Garcia and Igor Polikarpov

    Citation: Biotechnology for Biofuels 2011 4:54

    Content type: Research

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  3. Mixtures of prairie species (mixed prairie species; MPS) have been proposed to offer important advantages as a feedstock for sustainable production of fuels and chemicals. Therefore, understanding the performa...

    Authors: Jaclyn D DeMartini and Charles E Wyman

    Citation: Biotechnology for Biofuels 2011 4:52

    Content type: Research

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  4. The description of new hydrolytic enzymes is an important step in the development of techniques which use lignocellulosic materials as a starting point for fuel production. Sugarcane bagasse, which is subjecte...

    Authors: Severino A Lucena, Leile S Lima, Luís SA Cordeiro Jr, Celso Sant'Anna, Reginaldo Constantino, Patricia Azambuja, Wanderley de Souza, Eloi S Garcia and Fernando A Genta

    Citation: Biotechnology for Biofuels 2011 4:51

    Content type: Research

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  5. Lignocellulosic materials have been moved towards the forefront of the biofuel industry as a sustainable resource. However, saccharification and the production of bioproducts derived from plant cell wall bioma...

    Authors: João Paulo L Franco Cairo, Flávia C Leonardo, Thabata M Alvarez, Daniela A Ribeiro, Fernanda Büchli, Ana M Costa-Leonardo, Marcelo F Carazzolle, Fernando F Costa, Adriana F Paes Leme, Gonçalo AG Pereira and Fabio M Squina

    Citation: Biotechnology for Biofuels 2011 4:50

    Content type: Research

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  6. Softwoods are the dominant source of lignocellulosic biomass in the northern hemisphere, and have been investigated worldwide as a renewable substrate for cellulosic ethanol production. One challenge to using ...

    Authors: Gary M Hawkins and Joy Doran-Peterson

    Citation: Biotechnology for Biofuels 2011 4:49

    Content type: Research

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  7. Recently developed iron cocatalyst enhancement of dilute acid pretreatment of biomass is a promising approach for enhancing sugar release from recalcitrant lignocellulosic biomass. However, very little is know...

    Authors: Hui Wei, Bryon S Donohoe, Todd B Vinzant, Peter N Ciesielski, Wei Wang, Lynn M Gedvilas, Yining Zeng, David K Johnson, Shi-You Ding, Michael E Himmel and Melvin P Tucker

    Citation: Biotechnology for Biofuels 2011 4:48

    Content type: Research

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  8. Biodiesel is considered to be a promising future substitute for fossil fuels, and microalgae are one source of biodiesel. The ratios of lipid, carbohydrates and proteins are different in different microalgal s...

    Authors: Cheng Yuan, Junhan Liu, Yong Fan, Xiaohui Ren, Guangrong Hu and Fuli Li

    Citation: Biotechnology for Biofuels 2011 4:47

    Content type: Research

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  9. In the normal process of bioethanol production, biomass is transported to integrated large factories for degradation to sugar, fermentation, and recovery of ethanol by distillation. Biomass nutrient loss occur...

    Authors: Hiroko K Kitamoto, Mitsuo Horita, Yimin Cai, Yukiko Shinozaki and Keiji Sakaki

    Citation: Biotechnology for Biofuels 2011 4:46

    Content type: Research

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  10. Lignin is a highly abundant biopolymer synthesized by plants as a complex component of plant secondary cell walls. Efforts to utilize lignin-based bioproducts are needed.

    Authors: Venugopal Mendu, Anne E Harman-Ware, Mark Crocker, Jungho Jae, Jozsef Stork, Samuel Morton III, Andrew Placido, George Huber and Seth DeBolt

    Citation: Biotechnology for Biofuels 2011 4:43

    Content type: Research

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  11. The enzymatic production of biodiesel through alcoholysis of triglycerides has become more attractive because it shows potential in overcoming the drawbacks of chemical processes. In this study, we investigate...

    Authors: Koei Kawakami, Yasuhiro Oda and Ryo Takahashi

    Citation: Biotechnology for Biofuels 2011 4:42

    Content type: Research

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  12. In converting biomass to bioethanol, pretreatment is a key step intended to render cellulose more amenable and accessible to cellulase enzymes and thus increase glucose yields. In this study, four cellulose sa...

    Authors: Ashutosh Mittal, Rui Katahira, Michael E Himmel and David K Johnson

    Citation: Biotechnology for Biofuels 2011 4:41

    Content type: Research

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  13. Considering that the costs of cellulases and hemicellulases contribute substantially to the price of bioethanol, new studies aimed at understanding and improving cellulase efficiency and productivity are of pa...

    Authors: Wagner R de Souza, Paula F de Gouvea, Marcela Savoldi, Iran Malavazi, Luciano A de Souza Bernardes, Maria Helena S Goldman, Ronald P de Vries, Juliana V de Castro Oliveira and Gustavo H Goldman

    Citation: Biotechnology for Biofuels 2011 4:40

    Content type: Research

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  14. Hydrolysates of plant biomass used for the production of lignocellulosic biofuels typically contain sugar mixtures consisting mainly of D-glucose and D-xylose, and minor amounts of L-arabinose. The yeast Saccharo...

    Authors: Thorsten Subtil and Eckhard Boles

    Citation: Biotechnology for Biofuels 2011 4:38

    Content type: Research

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  15. Irpex lacteus, a versatile lignin-degrading fungus with various extracellular enzymes, has been widely used for biological pretreatment. However, most studies have focused on the change of substrate structure aft...

    Authors: Wanqing Du, Hongbo Yu, Lili Song, Ji Zhang, Changlong Weng, Fuying Ma and Xiaoyu Zhang

    Citation: Biotechnology for Biofuels 2011 4:37

    Content type: Research

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  16. We and other workers have shown that accessory enzymes, such as β-glucosidase, xylanase, and cellulase cofactors, such as GH61, can considerably enhance the hydrolysis effectiveness of cellulase cocktails when...

    Authors: Jinguang Hu, Valdeir Arantes and Jack N Saddler

    Citation: Biotechnology for Biofuels 2011 4:36

    Content type: Research

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  17. Ethanol production from paper sludge (PS) by simultaneous saccharification and fermentation (SSF) is considered to be the most appropriate way to process PS, as it contains negligible lignin. In this study, SS...

    Authors: Joni Prasetyo, Kazuya Naruse, Tatsuya Kato, Chuenchit Boonchird, Satoshi Harashima and Enoch Y Park

    Citation: Biotechnology for Biofuels 2011 4:35

    Content type: Research

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  18. Microalgae are a promising feedstock for biofuel and bioenergy production due to their high photosynthetic efficiencies, high growth rates and no need for external organic carbon supply. In this study, utiliza...

    Authors: Aino-Maija Lakaniemi, Christopher J Hulatt, David N Thomas, Olli H Tuovinen and Jaakko A Puhakka

    Citation: Biotechnology for Biofuels 2011 4:34

    Content type: Research

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  19. In order to generate biofuels, insoluble cellulosic substrates are pretreated andsubsequently hydrolyzed with cellulases. One way to pretreat cellulose in a safeand environmentally friendly manner is to apply,...

    Authors: Gernot Jäger, Michele Girfoglio, Florian Dollo, Roberto Rinaldi, Hans Bongard, Ulrich Commandeur, Rainer Fischer, Antje C Spiess and Jochen Büchs

    Citation: Biotechnology for Biofuels 2011 4:33

    Content type: Research

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  20. Caldicellulosiruptor saccharolyticus has attracted increased interest as an industrial hydrogen (H2) producer. The aim of the present study was to develop a kinetic growth model for this extreme thermophile. The ...

    Authors: Mattias Ljunggren, Karin Willquist, Guido Zacchi and Ed WJ van Niel

    Citation: Biotechnology for Biofuels 2011 4:31

    Content type: Research

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  21. The main technological impediment to widespread utilization of lignocellulose for the production of fuels and chemicals is the lack of low-cost technologies to overcome its recalcitrance. Organisms that hydrol...

    Authors: Marja Ilmén, Riaan den Haan, Elena Brevnova, John McBride, Erin Wiswall, Allan Froehlich, Anu Koivula, Sanni P Voutilainen, Matti Siika-aho, Daniël C la Grange, Naomi Thorngren, Simon Ahlgren, Mark Mellon, Kristen Deleault, Vineet Rajgarhia, Willem H van Zyl…

    Citation: Biotechnology for Biofuels 2011 4:30

    Content type: Research

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  22. Cellulases and related hydrolytic enzymes represent a key cost factor for biochemical conversion of cellulosic biomass feedstocks to sugars for biofuels and chemicals production. The US Department of Energy (D...

    Authors: James D McMillan, Edward W Jennings, Ali Mohagheghi and Mildred Zuccarello

    Citation: Biotechnology for Biofuels 2011 4:29

    Content type: Research

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  23. Atmospheric pressure chemical ionisation mass spectrometry (APCI-MS) offers advantages as a rapid analytical technique for the quantification of three biomass degradation products (acetic acid, formic acid and...

    Authors: Scott M Davies, Rob S Linforth, Stuart J Wilkinson, Katherine A Smart and David J Cook

    Citation: Biotechnology for Biofuels 2011 4:28

    Content type: Methodology

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  24. The trichothecene mycotoxin deoxynivalenol (DON) may be concentrated in distillers dried grains with solubles (DDGS; a co-product of fuel ethanol fermentation) when grain containing DON is used to produce fuel...

    Authors: Piyum A Khatibi, Justin Montanti, Nhuan P Nghiem, Kevin B Hicks, Greg Berger, Wynse S Brooks, Carl A Griffey and David G Schmale III

    Citation: Biotechnology for Biofuels 2011 4:26

    Content type: Research

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  25. Biochemical conversion of lignocellulose hydrolysates remains challenging, largely because most microbial processes have markedly reduced efficiency in the presence of both hexoses and pentoses. Thus, identifi...

    Authors: Cuimin Hu, Siguo Wu, Qian Wang, Guojie Jin, Hongwei Shen and Zongbao K Zhao

    Citation: Biotechnology for Biofuels 2011 4:25

    Content type: Research

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  26. Neocallimastix patriciarum is one of the common anaerobic fungi in the digestive tracts of ruminants that can actively digest cellulosic materials, and its cellulases have great potential for hydrolyzing cellulos...

    Authors: Tzi-Yuan Wang, Hsin-Liang Chen, Mei-Yeh J Lu, Yo-Chia Chen, Huang-Mo Sung, Chi-Tang Mao, Hsing-Yi Cho, Huei-Mien Ke, Teh-Yang Hwa, Sz-Kai Ruan, Kuo-Yen Hung, Chih-Kuan Chen, Jeng-Yi Li, Yueh-Chin Wu, Yu-Hsiang Chen, Shao-Pei Chou…

    Citation: Biotechnology for Biofuels 2011 4:24

    Content type: Research

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  27. To efficiently deconstruct recalcitrant plant biomass to fermentable sugars in industrial processes, biocatalysts of higher performance and lower cost are required. The genetic diversity found in the metagenom...

    Authors: Luen-Luen Li, Safiyh Taghavi, Sean M McCorkle, Yian-Biao Zhang, Michael G Blewitt, Roman Brunecky, William S Adney, Michael E Himmel, Phillip Brumm, Colleen Drinkwater, David A Mead, Susannah G Tringe and Daniel van der Lelie

    Citation: Biotechnology for Biofuels 2011 4:23

    Content type: Research

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  28. As the supply of starch grain and sugar cane, currently the main feedstocks for bioethanol production, become limited, lignocelluloses will be sought as alternative materials for bioethanol production. Product...

    Authors: Yong Tang, Danqing Zhao, Carrasco Cristhian and Jianxin Jiang

    Citation: Biotechnology for Biofuels 2011 4:22

    Content type: Research

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  29. Isobutanol can be a better biofuel than ethanol due to its higher energy density and lower hygroscopicity. Furthermore, the branched-chain structure of isobutanol gives a higher octane number than the isomeric n-...

    Authors: Xiao Chen, Kristian F Nielsen, Irina Borodina, Morten C Kielland-Brandt and Kaisa Karhumaa

    Citation: Biotechnology for Biofuels 2011 4:21

    Content type: Research

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  30. The use of energy crops and agricultural residues is expected to increase to fulfil the legislative demands of bio-based components in transport fuels. Ensiling methods, adapted from the feed sector, are suita...

    Authors: Annukka Pakarinen, Pekka Maijala, Seija Jaakkola, Frederick L Stoddard, Maritta Kymäläinen and Liisa Viikari

    Citation: Biotechnology for Biofuels 2011 4:20

    Content type: Research

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  31. The BioEnergy Science Center (BESC) developed a high-throughput screening method to rapidly identify low-recalcitrance biomass variants. Because the customary separation and analysis of liquid and solids betwe...

    Authors: Michael H Studer, Simone Brethauer, Jaclyn D DeMartini, Heather L McKenzie and Charles E Wyman

    Citation: Biotechnology for Biofuels 2011 4:19

    Content type: Research

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  32. Hemicellulose is often credited with being one of the important physical barriers to enzymatic hydrolysis of cellulose, and acts by blocking enzyme access to the cellulose surface. In addition, our recent rese...

    Authors: Qing Qing and Charles E Wyman

    Citation: Biotechnology for Biofuels 2011 4:18

    Content type: Research

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  33. Termites are highly effective at degrading lignocelluloses, and thus can be used as a model for studying plant cell-wall degradation in biological systems. However, the process of lignin deconstruction and/or ...

    Authors: Jing Ke, Dhrubojyoti D Laskar, Deepak Singh and Shulin Chen

    Citation: Biotechnology for Biofuels 2011 4:17

    Content type: Research

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  34. Pretreatment is a critical step in the conversion of lignocellulose to fermentable sugars. Although many pretreatment processes are currently under investigation, none of them are entirely satisfactory in rega...

    Authors: Goutami Banerjee, Suzana Car, John S Scott-Craig, David B Hodge and Jonathan D Walton

    Citation: Biotechnology for Biofuels 2011 4:16

    Content type: Research

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  35. The recent energy crisis has triggered significant attention on the microbial synthesis of lipids, which comprise the raw material for biodiesel production. Microbial oil accumulation with filamentous fungi ha...

    Authors: Chunjie Xia, Jianguo Zhang, Weidong Zhang and Bo Hu

    Citation: Biotechnology for Biofuels 2011 4:15

    Content type: Research

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  36. Biomass use for the production of bioethanol or platform chemicals requires efficient breakdown of biomass to fermentable monosaccharides. Lignocellulosic feedstocks often require physicochemical pretreatment ...

    Authors: Stefan Kühnel, Henk A Schols and Harry Gruppen

    Citation: Biotechnology for Biofuels 2011 4:14

    Content type: Research

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  37. Short rotation coppice willow is a potential lignocellulosic feedstock in the United Kingdom and elsewhere; however, research on optimising willow specifically for bioethanol production has started developing ...

    Authors: Nicholas JB Brereton, Frederic E Pitre, Michael J Ray, Angela Karp and Richard J Murphy

    Citation: Biotechnology for Biofuels 2011 4:13

    Content type: Research

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  38. In the hydrolysis of lignocellulosic materials, thermostable enzymes decrease the amount of enzyme needed due to higher specific activity and elongate the hydrolysis time due to improved stability. For cost-ef...

    Authors: Junhua Zhang, Matti Siika-aho, Terhi Puranen, Ming Tang, Maija Tenkanen and Liisa Viikari

    Citation: Biotechnology for Biofuels 2011 4:12

    Content type: Research

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  39. The recent development of improved enzymes and pentose-using yeast for cellulosic ethanol processes calls for new attention to the lignocellulose pretreatment step. This study assessed the influence of pretrea...

    Authors: Mads Pedersen, Katja S Johansen and Anne S Meyer

    Citation: Biotechnology for Biofuels 2011 4:11

    Content type: Research

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  40. When scaling up lignocellulose-based ethanol production, the desire to increase the final ethanol titer after fermentation can introduce problems. A high concentration of water-insoluble solids (WIS) is needed...

    Authors: Benny Palmqvist, Magnus Wiman and Gunnar Lidén

    Citation: Biotechnology for Biofuels 2011 4:10

    Content type: Research

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  41. Xylose isomerase (XI) catalyses the isomerisation of xylose to xylulose in bacteria and some fungi. Currently, only a limited number of XI genes have been functionally expressed in Saccharomyces cerevisiae, the m...

    Authors: Nádia Skorupa Parachin and Marie F Gorwa-Grauslund

    Citation: Biotechnology for Biofuels 2011 4:9

    Content type: Research

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  42. Hydrolysis of cellulose requires the action of the cellulolytic enzymes endoglucanase, cellobiohydrolase and β-glucosidase. The expression ratios and synergetic effects of these enzymes significantly influence...

    Authors: Ryosuke Yamada, Naho Taniguchi, Tsutomu Tanaka, Chiaki Ogino, Hideki Fukuda and Akihiko Kondo

    Citation: Biotechnology for Biofuels 2011 4:8

    Content type: Research

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  43. Lignin and hemicelluloses are the major components limiting enzyme infiltration into cell walls. Determination of the topochemical distribution of lignin and aromatics in sugar cane might provide important dat...

    Authors: Germano Siqueira, Adriane MF Milagres, Walter Carvalho, Gerald Koch and André Ferraz

    Citation: Biotechnology for Biofuels 2011 4:7

    Content type: Research

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  44. Enzymatic biodiesel production by transesterification in solvent media has been investigated intensively, but glycerol, as a by-product, could block the immobilized enzyme and excess n-hexane, as a solution aid, ...

    Authors: Yonghong Meng, Guili Wang, Na Yang, Zhiqi Zhou, Yuejuan Li, Xiaomei Liang, Jinnan Chen, Ying Li and Jilun Li

    Citation: Biotechnology for Biofuels 2011 4:6

    Content type: Research

    Published on: