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  1. Historically, acid pretreatment technology for the production of bio-ethanol from corn stover has required severe conditions to overcome biomass recalcitrance. However, the high usage of acid and steam at seve...

    Authors: Xiaowen Chen, Ling Tao, Joseph Shekiro, Ali Mohaghaghi, Steve Decker, Wei Wang, Holly Smith, Sunkyu Park, Michael E Himmel and Melvin Tucker

    Citation: Biotechnology for Biofuels 2012 5:60

    Content type: Research

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  2. Lignocellulose is the most abundant biomass on earth. However, biomass recalcitrance has become a major factor affecting biofuel production. Although cellulose crystallinity significantly influences biomass sa...

    Authors: Ning Xu, Wei Zhang, Shuangfeng Ren, Fei Liu, Chunqiao Zhao, Haofeng Liao, Zhengdan Xu, Jiangfeng Huang, Qing Li, Yuanyuan Tu, Bin Yu, Yanting Wang, Jianxiong Jiang, Jingping Qin and Liangcai Peng

    Citation: Biotechnology for Biofuels 2012 5:58

    Content type: Research

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  3. A common trend in the research on 2nd generation bioethanol is the focus on intensifying the process and increasing the concentration of water insoluble solids (WIS) throughout the process. However, increasing th...

    Authors: Benny Palmqvist and Gunnar Lidén

    Citation: Biotechnology for Biofuels 2012 5:57

    Content type: Research

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  4. Because many Miscanthus genotypes can be cultivated with relatively high productivity and carbohydrate content, Miscanthus has great potential as an energy crop that can support large scale biological production ...

    Authors: Taiying Zhang, Charles E Wyman, Katrin Jakob and Bin Yang

    Citation: Biotechnology for Biofuels 2012 5:56

    Content type: Research

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  5. Degradation of cellulose to glucose requires the cooperative action of three classes of enzymes, collectively known as cellulases. Endoglucanases randomly bind to cellulose surfaces and generate new chain ends...

    Authors: Andrea Asztalos, Marcus Daniels, Anurag Sethi, Tongye Shen, Paul Langan, Antonio Redondo and Sandrasegaram Gnanakaran

    Citation: Biotechnology for Biofuels 2012 5:55

    Content type: Research

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  6. Thermophilic fungi have attracted increased interest for their ability to secrete enzymes that deconstruct biomass at high temperatures. However, development of thermophilic fungi as enzyme producers for bioma...

    Authors: Shara D McClendon, Tanveer Batth, Christopher J Petzold, Paul D Adams, Blake A Simmons and Steven W Singer

    Citation: Biotechnology for Biofuels 2012 5:54

    Content type: Research

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  7. To achieve an economical cellulosic ethanol production, a host that can do both cellulosic saccharification and ethanol fermentation is desirable. However, to engineer a non-cellulolytic yeast to be such a hos...

    Authors: Jui-Jen Chang, Cheng-Yu Ho, Feng-Ju Ho, Tsung-Yu Tsai, Huei-Mien Ke, Christine H-T Wang, Hsin-Liang Chen, Ming-Che Shih, Chieh-Chen Huang and Wen-Hsiung Li

    Citation: Biotechnology for Biofuels 2012 5:53

    Content type: Research

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  8. Fungi are important players in the turnover of plant biomass because they produce a broad range of degradative enzymes. Aspergillus nidulans, a well-studied saprophyte and close homologue to industrially importan...

    Authors: Sayali Saykhedkar, Anamika Ray, Patricia Ayoubi-Canaan, Steven D Hartson, Rolf Prade and Andrew J Mort

    Citation: Biotechnology for Biofuels 2012 5:52

    Content type: Research

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  9. Cellulose amorphogenesis, described as the non-hydrolytic “opening up” or disruption of a cellulosic substrate, is becoming increasingly recognized as one of the key steps in the enzymatic deconstruction of ce...

    Authors: Keith Gourlay, Valdeir Arantes and Jack N Saddler

    Citation: Biotechnology for Biofuels 2012 5:51

    Content type: Methodology

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  10. Lipids produced from filamentous fungi show great promise for biofuel production, but a major limiting factor is the high production cost attributed to feedstock. Lignocellulosic biomass is a suitable feedstoc...

    Authors: Yubin Zheng, Xiaochen Yu, Jijiao Zeng and Shulin Chen

    Citation: Biotechnology for Biofuels 2012 5:50

    Content type: Research

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  11. The impact of hydrothermal flowthrough (FT) pretreatment severity on pretreatment and solubilization performance metrics was evaluated for three milled feedstocks (corn stover, bagasse, and poplar) and two con...

    Authors: Veronique Archambault-Leger, Xiongjun Shao and Lee R Lynd

    Citation: Biotechnology for Biofuels 2012 5:49

    Content type: Research

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  12. The considerable increase in biodiesel production worldwide in the last 5 years resulted in a stoichiometric increased coproduction of crude glycerol. As an excess of crude glycerol has been produced, its valu...

    Authors: João R M Almeida, Léia C L Fávaro and Betania F Quirino

    Citation: Biotechnology for Biofuels 2012 5:48

    Content type: Review

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  13. Temporal and spatial expression of fatty acid and lipid biosynthetic genes are associated with the accumulation of storage lipids in the seeds of oil plants. In jatropha (Jatropha curcas L.), a potential biofuel ...

    Authors: Keyu Gu, Chengxin Yi, Dongsheng Tian, Jatinder Singh Sangha, Yan Hong and Zhongchao Yin

    Citation: Biotechnology for Biofuels 2012 5:47

    Content type: Research

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  14. The bulk terrestrial biomass resource in a future bio-economy will be lignocellulosic biomass, which is recalcitrant and challenging to process. Enzymatic conversion of polysaccharides in the lignocellulosic b...

    Authors: Svein Jarle Horn, Gustav Vaaje-Kolstad, Bjørge Westereng and VincentGH Eijsink

    Citation: Biotechnology for Biofuels 2012 5:45

    Content type: Review

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  15. Previously we have developed a butanol tolerant mutant of Clostridium acetobutylicum Rh8, from the wild type strain DSM 1731. Strain Rh8 can tolerate up to 19 g/L butanol, with solvent titer improved accordingly,...

    Authors: Zongjie Dai, Hongjun Dong, Yan Zhu, Yanping Zhang, Yin Li and Yanhe Ma

    Citation: Biotechnology for Biofuels 2012 5:44

    Content type: Research

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  16. A previously developed mathematical model of low solids thermophilic simultaneous saccharification and fermentation (tSSF) with Avicel was unable to predict performance at high solids using a commercial cellul...

    Authors: Kara K Podkaminer, William R Kenealy, Christopher D Herring, David A Hogsett and Lee R Lynd

    Citation: Biotechnology for Biofuels 2012 5:43

    Content type: Research

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  17. Higher seed yield is one of the objectives of jatropha breeding. However, genetic analysis of the yield traits has not been done in jatropha. Quantitative trait loci (QTL) mapping was conducted to identify gen...

    Authors: Fei Sun, Peng Liu, Jian Ye, Loong Chuan Lo, Suying Cao, Lei Li, Gen Hua Yue and Chun Ming Wang

    Citation: Biotechnology for Biofuels 2012 5:42

    Content type: Research

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  18. Renewable energy production is currently a major issue worldwide. Biogas is a promising renewable energy carrier as the technology of its production combines the elimination of organic waste with the formation...

    Authors: Roland Wirth, Etelka Kovács, Gergely Maróti, Zoltán Bagi, Gábor Rákhely and Kornél L Kovács

    Citation: Biotechnology for Biofuels 2012 5:41

    Content type: Research

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  19. Phaeodactylum tricornutum is a unicellular diatom in the class Bacillariophyceae. The full genome has been sequenced (<30 Mb), and approximately 20 to 30% triacylglyceride (TAG) accumulation on a dry cell basis h...

    Authors: Jacob Valenzuela, Aurelien Mazurie, Ross P Carlson, Robin Gerlach, Keith E Cooksey, Brent M Peyton and Matthew W Fields

    Citation: Biotechnology for Biofuels 2012 5:40

    Content type: Research

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  20. Presently, different studies are conducted related to the topic of biomass potential to generate through anaerobic fermentation process alternative fuels supposed to support the existing fossil fuel resources,...

    Authors: Adrian Eugen Cioabla, Ioana Ionel, Gabriela-Alina Dumitrel and Francisc Popescu

    Citation: Biotechnology for Biofuels 2012 5:39

    Content type: Research

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  21. For cellulosic biofuels processes, suitable characterization of the lignin remaining within the cell wall and correlation of quantified properties of lignin to cell wall polysaccharide enzymatic deconstruction...

    Authors: Muyang Li, Cliff Foster, Shantanu Kelkar, Yunqiao Pu, Daniel Holmes, Arthur Ragauskas, Christopher M Saffron and David B Hodge

    Citation: Biotechnology for Biofuels 2012 5:38

    Content type: Research

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  22. Switchgrass is one of the most promising bioenergy crop candidates for the US. It gives relatively high biomass yield and can grow on marginal lands. However, its yields vary from year to year and from locatio...

    Authors: Seonhwa Kim, Scott Lowman, Guichuan Hou, Jerzy Nowak, Barry Flinn and Chuansheng Mei

    Citation: Biotechnology for Biofuels 2012 5:37

    Content type: Research

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  23. Previous studies on the use of SO2 and CO2 as impregnating agent for sugar cane bagasse steam treatment showed comparative and promising results concerning the cellulose enzymatic hydrolysis and the low formation...

    Authors: Roberta Cristina Novaes Reis Corrales, Fabiana Magalhães Teixeira Mendes, Clarissa Cruz Perrone, Celso Sant’Anna, Wanderley de Souza, Yuri Abud, Elba Pinto Pinto da Silva Bon and Viridiana Ferreira-Leitão

    Citation: Biotechnology for Biofuels 2012 5:36

    Content type: Research

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  24. Pure bacterial strains give better yields when producing H2 than mixed, natural communities. However the main drawback with the pure cultures is the need to perform the fermentations under sterile conditions. The...

    Authors: Julien Masset, Magdalena Calusinska, Christopher Hamilton, Serge Hiligsmann, Bernard Joris, Annick Wilmotte and Philippe Thonart

    Citation: Biotechnology for Biofuels 2012 5:35

    Content type: Research

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  25. The concerted effects of changes in gene expression due to changes in the environment are ultimately reflected in the metabolome. Dynamics of metabolite concentrations under a certain condition can therefore g...

    Authors: Basti Bergdahl, Dominik Heer, Uwe Sauer, Bärbel Hahn-Hägerdal and Ed WJ van Niel

    Citation: Biotechnology for Biofuels 2012 5:34

    Content type: Research

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  26. The desulphurization of dibenzothiophene (DBT), a recalcitrant thiophenic fossil fuel component by Serratia marcescens (UCP 1549) in order for reducing the Sulphur content was investigated. The Study was carried ...

    Authors: HélviaW Casullo de Araújo, Marta Cristina de Freitas Siva, Clarissai Matos Lins, Aline Elesbão do Nascimento, CarlosAlberto Alves da Silva and Galba M Campos-Takaki

    Citation: Biotechnology for Biofuels 2012 5:33

    Content type: Research

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  27. One of the crucial factors for a sustainable and economical production of lignocellulosic based bioethanol is the availability of a robust fermenting microorganism with high tolerance to inhibitors generated d...

    Authors: Rakesh Koppram, Eva Albers and Lisbeth Olsson

    Citation: Biotechnology for Biofuels 2012 5:32

    Content type: Research

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  28. β-Glucosidase is an important component of the cellulase enzyme system. It does not only participate in cellulose degradation, it also plays an important role in hydrolyzing cellulose to fermentable glucose by...

    Authors: Jianjun Pei, Qian Pang, Linguo Zhao, Song Fan and Hao Shi

    Citation: Biotechnology for Biofuels 2012 5:31

    Content type: Research

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  29. The microbial bioemulsifiers was surface active compounds, are more effective in stabilizing oil-in-water emulsions. The yeasts have been isolated to produce bioemulsifiers from vegetable oils and industrial w...

    Authors: Andrea de Souza Monteiro, Vitor Souza Domingues, Marcus VD Souza, Ivana Lula, Daniel Bonoto Gonçalves, Ezequias Pessoa de Siqueira and Vera Lúcia dos Santos

    Citation: Biotechnology for Biofuels 2012 5:29

    Content type: Research

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  30. A major challenge in the identification and development of superior feedstocks for the production of second generation biofuels is the rapid assessment of biomass composition in a large number of samples. Curr...

    Authors: German Muttoni, James M Johnson, Nicholas Santoro, Craig J Rhiner, Karl J Haro von Mogel, Shawn M Kaeppler and Natalia de Leon

    Citation: Biotechnology for Biofuels 2012 5:27

    Content type: Methodology

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  31. The recent discovery of accessory proteins that boost cellulose hydrolysis has increased the economical and technical efficiency of processing cellulose to bioethanol. Oxidative enzymes (e.g. GH61) present in ...

    Authors: David Cannella, Chia-wen C Hsieh, Claus Felby and Henning Jørgensen

    Citation: Biotechnology for Biofuels 2012 5:26

    Content type: Research

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  32. Cellulose, which is the most abundant renewable biomass on earth, is a potential bio-resource of alternative energy. The hydrolysis of plant polysaccharides is catalyzed by microbial cellulases, including endo...

    Authors: Hsin-Liang Chen, Yo-Chia Chen, Mei-Yeh Jade Lu, Jui-Jen Chang, Hiaow-Ting Christine Wang, Huei-Mien Ke, Tzi-Yuan Wang, Sz-Kai Ruan, Tao-Yuan Wang, Kuo-Yen Hung, Hsing-Yi Cho, Wan-Ting Lin, Ming-Che Shih and Wen-Hsiung Li

    Citation: Biotechnology for Biofuels 2012 5:24

    Content type: Research

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  33. Cost-effective production of lignocellulosic biofuels remains a major financial and technical challenge at the industrial scale. A critical tool in biofuels process development is the techno-economic (TE) mode...

    Authors: Kristin J Vicari, Sai Sandeep Tallam, Tatyana Shatova, Koh Kang Joo, Christopher J Scarlata, David Humbird, Edward J Wolfrum and Gregg T Beckham

    Citation: Biotechnology for Biofuels 2012 5:23

    Content type: Research

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  34. Bioethanol produced from the lignocellulosic fractions of sugar cane (bagasse and leaves), i.e. second generation (2G) bioethanol, has a promising market potential as an automotive fuel; however, the process i...

    Authors: Stefano Macrelli, Johan Mogensen and Guido Zacchi

    Citation: Biotechnology for Biofuels 2012 5:22

    Content type: Research

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  35. Cellulose consisting of arrays of linear beta-1,4 linked glucans, is the most abundant carbon-containing polymer present in biomass. Recalcitrance of crystalline cellulose towards enzymatic degradation is wide...

    Authors: Fernando Segato, André R L Damasio, Thiago Augusto Gonçalves, Mario T Murakami, Fabio M Squina, MariadeLourdesTM Polizeli, Andrew J Mort and Rolf A Prade

    Citation: Biotechnology for Biofuels 2012 5:21

    Content type: Research

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  36. Understanding the dynamics of the microbial communities that, along with their secreted enzymes, are involved in the natural process of biomass composting may hold the key to breaking the major bottleneck in b...

    Authors: Hui Wei, Melvin P Tucker, John O Baker, Michelle Harris, Yonghua Luo, Qi Xu, Michael E Himmel and Shi-You Ding

    Citation: Biotechnology for Biofuels 2012 5:20

    Content type: Research

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  37. Lipids extracted from seeds of Camelina sativa have been successfully used as a reliable source of aviation biofuels. This biofuel is environmentally friendly because the drought resistance, frost tolerance and l...

    Authors: Youjun Zhang, Laura Yu, Ka-Fu Yung, Dennis YC Leung, Feng Sun and Boon L Lim

    Citation: Biotechnology for Biofuels 2012 5:19

    Content type: Research

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  38. Biofuel has been the focus of intensive global research over the past few years. The development of 4th generation biofuel production (algae-to-biofuels) based on metabolic engineering of algae is still in its in...

    Authors: Anping Lei, Huan Chen, Guoming Shen, Zhangli Hu, Lei Chen and Jiangxin Wang

    Citation: Biotechnology for Biofuels 2012 5:18

    Content type: Research

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  39. Direct conversion of solar energy and carbon dioxide to drop in fuel molecules in a single biological system can be achieved from fatty acid-based biofuels such as fatty alcohols and alkanes. These molecules h...

    Authors: Qianqian Gao, Weihua Wang, Hui Zhao and Xuefeng Lu

    Citation: Biotechnology for Biofuels 2012 5:17

    Content type: Research

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  40. Enzymatic hydrolysis, the rate limiting step in the process development for biofuel, is always hampered by its low sugar concentration. High solid enzymatic saccharification could solve this problem but has se...

    Authors: Rishi Gupta, Sanjay Kumar, James Gomes and Ramesh Chander Kuhad

    Citation: Biotechnology for Biofuels 2012 5:16

    Content type: Research

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  41. The commercialization of second-generation bioethanol has not been realized due to several factors, including poor biomass utilization and high production cost. It is generally accepted that the most important...

    Authors: Borbála Erdei, Balázs Frankó, Mats Galbe and Guido Zacchi

    Citation: Biotechnology for Biofuels 2012 5:12

    Content type: Research

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  42. Jatropha curcas is recognized as a new energy crop due to the presence of the high amount of oil in its seeds that can be converted into biodiesel. The quality and performance of the biodiesel depends on the chem...

    Authors: Jing Qu, Hui-Zhu Mao, Wen Chen, Shi-Qiang Gao, Ya-Nan Bai, Yan-Wei Sun, Yun-Feng Geng and Jian Ye

    Citation: Biotechnology for Biofuels 2012 5:10

    Content type: Research

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