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  1. Cellulose, which is the most abundant renewable biomass on earth, is a potential bio-resource of alternative energy. The hydrolysis of plant polysaccharides is catalyzed by microbial cellulases, including endo...

    Authors: Hsin-Liang Chen, Yo-Chia Chen, Mei-Yeh Jade Lu, Jui-Jen Chang, Hiaow-Ting Christine Wang, Huei-Mien Ke, Tzi-Yuan Wang, Sz-Kai Ruan, Tao-Yuan Wang, Kuo-Yen Hung, Hsing-Yi Cho, Wan-Ting Lin, Ming-Che Shih and Wen-Hsiung Li

    Citation: Biotechnology for Biofuels 2012 5:24

    Content type: Research

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  2. Cost-effective production of lignocellulosic biofuels remains a major financial and technical challenge at the industrial scale. A critical tool in biofuels process development is the techno-economic (TE) mode...

    Authors: Kristin J Vicari, Sai Sandeep Tallam, Tatyana Shatova, Koh Kang Joo, Christopher J Scarlata, David Humbird, Edward J Wolfrum and Gregg T Beckham

    Citation: Biotechnology for Biofuels 2012 5:23

    Content type: Research

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  3. Bioethanol produced from the lignocellulosic fractions of sugar cane (bagasse and leaves), i.e. second generation (2G) bioethanol, has a promising market potential as an automotive fuel; however, the process i...

    Authors: Stefano Macrelli, Johan Mogensen and Guido Zacchi

    Citation: Biotechnology for Biofuels 2012 5:22

    Content type: Research

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  4. Cellulose consisting of arrays of linear beta-1,4 linked glucans, is the most abundant carbon-containing polymer present in biomass. Recalcitrance of crystalline cellulose towards enzymatic degradation is wide...

    Authors: Fernando Segato, André R L Damasio, Thiago Augusto Gonçalves, Mario T Murakami, Fabio M Squina, MariadeLourdesTM Polizeli, Andrew J Mort and Rolf A Prade

    Citation: Biotechnology for Biofuels 2012 5:21

    Content type: Research

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  5. Understanding the dynamics of the microbial communities that, along with their secreted enzymes, are involved in the natural process of biomass composting may hold the key to breaking the major bottleneck in b...

    Authors: Hui Wei, Melvin P Tucker, John O Baker, Michelle Harris, Yonghua Luo, Qi Xu, Michael E Himmel and Shi-You Ding

    Citation: Biotechnology for Biofuels 2012 5:20

    Content type: Research

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  6. Lipids extracted from seeds of Camelina sativa have been successfully used as a reliable source of aviation biofuels. This biofuel is environmentally friendly because the drought resistance, frost tolerance and l...

    Authors: Youjun Zhang, Laura Yu, Ka-Fu Yung, Dennis YC Leung, Feng Sun and Boon L Lim

    Citation: Biotechnology for Biofuels 2012 5:19

    Content type: Research

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  7. Biofuel has been the focus of intensive global research over the past few years. The development of 4th generation biofuel production (algae-to-biofuels) based on metabolic engineering of algae is still in its in...

    Authors: Anping Lei, Huan Chen, Guoming Shen, Zhangli Hu, Lei Chen and Jiangxin Wang

    Citation: Biotechnology for Biofuels 2012 5:18

    Content type: Research

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  8. Direct conversion of solar energy and carbon dioxide to drop in fuel molecules in a single biological system can be achieved from fatty acid-based biofuels such as fatty alcohols and alkanes. These molecules h...

    Authors: Qianqian Gao, Weihua Wang, Hui Zhao and Xuefeng Lu

    Citation: Biotechnology for Biofuels 2012 5:17

    Content type: Research

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  9. Enzymatic hydrolysis, the rate limiting step in the process development for biofuel, is always hampered by its low sugar concentration. High solid enzymatic saccharification could solve this problem but has se...

    Authors: Rishi Gupta, Sanjay Kumar, James Gomes and Ramesh Chander Kuhad

    Citation: Biotechnology for Biofuels 2012 5:16

    Content type: Research

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  10. The commercialization of second-generation bioethanol has not been realized due to several factors, including poor biomass utilization and high production cost. It is generally accepted that the most important...

    Authors: Borbála Erdei, Balázs Frankó, Mats Galbe and Guido Zacchi

    Citation: Biotechnology for Biofuels 2012 5:12

    Content type: Research

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  11. Jatropha curcas is recognized as a new energy crop due to the presence of the high amount of oil in its seeds that can be converted into biodiesel. The quality and performance of the biodiesel depends on the chem...

    Authors: Jing Qu, Hui-Zhu Mao, Wen Chen, Shi-Qiang Gao, Ya-Nan Bai, Yan-Wei Sun, Yun-Feng Geng and Jian Ye

    Citation: Biotechnology for Biofuels 2012 5:10

    Content type: Research

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  12. An efficient hydrolysis of lignocellulosic substrates to soluble sugars for biofuel production necessitates the interplay and synergistic interaction of multiple enzymes. An optimized enzyme mixture is crucial...

    Authors: Hélène Billard, Abdelaziz Faraj, Nicolas Lopes Ferreira, Sandra Menir and Senta Heiss-Blanquet

    Citation: Biotechnology for Biofuels 2012 5:9

    Content type: Research

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  13. Dilute acid pretreatment is a promising pretreatment technology for the biochemical production of ethanol from lignocellulosic biomass. During dilute acid pretreatment, xylan depolymerizes to form soluble xylo...

    Authors: Xiaowen Chen, Joseph Shekiro, Mary Ann Franden, Wei Wang, Min Zhang, Erik Kuhn, David K Johnson and Melvin P Tucker

    Citation: Biotechnology for Biofuels 2012 5:8

    Content type: Research

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  14. Wax ester synthases (WSs) can synthesize wax esters from alcohols and fatty acyl coenzyme A thioesters. The knowledge of the preferred substrates for each WS allows the use of yeast cells for the production of...

    Authors: Shuobo Shi, Juan Octavio Valle-Rodríguez, Sakda Khoomrung, Verena Siewers and Jens Nielsen

    Citation: Biotechnology for Biofuels 2012 5:7

    Content type: Research

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  15. Second generation hydrogen fermentation technologies using organic agricultural and forestry wastes are emerging. The efficient microbial fermentation of hexoses and pentoses resulting from the pretreatment of...

    Authors: Angela A Abreu, Dimitar Karakashev, Irini Angelidaki, Diana Z Sousa and M Madalena Alves

    Citation: Biotechnology for Biofuels 2012 5:6

    Content type: Research

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  16. There is currently considerable interest in developing renewable sources of energy. One strategy is the biological conversion of plant biomass to liquid transportation fuel. Several technical hurdles impinge u...

    Authors: Scott J Lee, Thomas A Warnick, Sivakumar Pattathil, Jesús G Alvelo-Maurosa, Michelle J Serapiglia, Heather McCormick, Virginia Brown, Naomi F Young, Danny J Schnell, Lawrence B Smart, Michael G Hahn, Jeffrey F Pedersen, Susan B Leschine and Samuel P Hazen

    Citation: Biotechnology for Biofuels 2012 5:5

    Content type: Research

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  17. Microbial lipids have drawn increasing attention in recent years as promising raw materials for biodiesel production, and the use of lignocellulosic hydrolysates as carbon sources seems to be a feasible strate...

    Authors: Chao Huang, Hong Wu, Zong-jun Liu, Jun Cai, Wen-yong Lou and Min-hua Zong

    Citation: Biotechnology for Biofuels 2012 5:4

    Content type: Research

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  18. Improving the hydrolytic performance of hemicellulases on lignocellulosic biomass is of considerable importance for second-generation biorefining. To address this problem, and also to gain greater understandin...

    Authors: Letian Song, Béatrice Siguier, Claire Dumon, Sophie Bozonnet and Michael J O'Donohue

    Citation: Biotechnology for Biofuels 2012 5:3

    Content type: Research

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  19. The model bacterium Clostridium cellulolyticum efficiently degrades crystalline cellulose and hemicellulose, using cellulosomes to degrade lignocellulosic biomass. Although it imports and ferments both pentose an...

    Authors: Yongchao Li, Timothy J Tschaplinski, Nancy L Engle, Choo Y Hamilton, Miguel Rodriguez Jr, James C Liao, Christopher W Schadt, Adam M Guss, Yunfeng Yang and David E Graham

    Citation: Biotechnology for Biofuels 2012 5:2

    Content type: Research

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  20. The ascomycete fungus, Trichoderma reesei (anamorph of Hypocrea jecorina), represents a biotechnological workhorse and is currently one of the most proficient cellulase producers. While strain improvement was tra...

    Authors: André Schuster, Kenneth S Bruno, James R Collett, Scott E Baker, Bernhard Seiboth, Christian P Kubicek and Monika Schmoll

    Citation: Biotechnology for Biofuels 2012 5:1

    Content type: Research

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  21. We describe a new selection method based on BODIPY (4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene) staining, fluorescence activated cell sorting (FACS) and microplate-based isolation of lipid-ric...

    Authors: Hugo Pereira, Luísa Barreira, André Mozes, Cláudia Florindo, Cristina Polo, Catarina V Duarte, Luísa Custódio and João Varela

    Citation: Biotechnology for Biofuels 2011 4:61

    Content type: Methodology

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  22. Due to the complexity of lignocellulosic materials, a complete enzymatic hydrolysis into fermentable sugars requires a variety of cellulolytic and xylanolytic enzymes. Addition of xylanases has been shown to s...

    Authors: Junhua Zhang, Matti Siika-aho, Maija Tenkanen and Liisa Viikari

    Citation: Biotechnology for Biofuels 2011 4:60

    Content type: Research

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  23. Contamination of bacteria in large-scale yeast fermentations is a serious problem and a threat to the development of successful biofuel production plants. Huge research efforts have been spent in order to solv...

    Authors: Eva Albers, Emma Johansson, Carl Johan Franzén and Christer Larsson

    Citation: Biotechnology for Biofuels 2011 4:59

    Content type: Research

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  24. Large-scale production of effective cellulose hydrolytic enzymes is the key to the bioconversion of agricultural residues to ethanol. The goal of this study was to develop a rice plant as a bioreactor for the ...

    Authors: Hong Li Chou, Ziyu Dai, Chia Wen Hsieh and Maurice SB Ku

    Citation: Biotechnology for Biofuels 2011 4:58

    Content type: Research

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  25. The optimization of industrial bioethanol production will depend on the rational design and manipulation of industrial strains to improve their robustness against the many stress factors affecting their perfor...

    Authors: Francisco B Pereira, Pedro MR Guimarães, Daniel G Gomes, Nuno P Mira, Miguel C Teixeira, Isabel Sá-Correia and Lucília Domingues

    Citation: Biotechnology for Biofuels 2011 4:57

    Content type: Research

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  26. Solid acid catalyst was prepared from Kraft lignin by chemical activation with phosphoric acid, pyrolysis and sulfuric acid. This catalyst had high acid density as characterized by scanning electron microscope...

    Authors: Fei-ling Pua, Zhen Fang, Sarani Zakaria, Feng Guo and Chin-hua Chia

    Citation: Biotechnology for Biofuels 2011 4:56

    Content type: Research

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    The Erratum to this article has been published in Biotechnology for Biofuels 2012 5:66

  27. The recalcitrance of lignocellulosic materials is a major limitation for their conversion into fermentable sugars. Lignin depletion in new cultivars or transgenic plants has been identified as a way to diminis...

    Authors: Fernando Masarin, Daniela B Gurpilhares, David CF Baffa, Márcio HP Barbosa, Walter Carvalho, André Ferraz and Adriane MF Milagres

    Citation: Biotechnology for Biofuels 2011 4:55

    Content type: Research

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  28. In recent years, biorefining of lignocellulosic biomass to produce multi-products such as ethanol and other biomaterials has become a dynamic research area. Pretreatment technologies that fractionate sugarcane...

    Authors: Camila Alves Rezende, Marisa Aparecida de Lima, Priscila Maziero, Eduardo Ribeiro deAzevedo, Wanius Garcia and Igor Polikarpov

    Citation: Biotechnology for Biofuels 2011 4:54

    Content type: Research

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  29. Mixtures of prairie species (mixed prairie species; MPS) have been proposed to offer important advantages as a feedstock for sustainable production of fuels and chemicals. Therefore, understanding the performa...

    Authors: Jaclyn D DeMartini and Charles E Wyman

    Citation: Biotechnology for Biofuels 2011 4:52

    Content type: Research

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  30. The description of new hydrolytic enzymes is an important step in the development of techniques which use lignocellulosic materials as a starting point for fuel production. Sugarcane bagasse, which is subjecte...

    Authors: Severino A Lucena, Leile S Lima, Luís SA Cordeiro Jr, Celso Sant'Anna, Reginaldo Constantino, Patricia Azambuja, Wanderley de Souza, Eloi S Garcia and Fernando A Genta

    Citation: Biotechnology for Biofuels 2011 4:51

    Content type: Research

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  31. Lignocellulosic materials have been moved towards the forefront of the biofuel industry as a sustainable resource. However, saccharification and the production of bioproducts derived from plant cell wall bioma...

    Authors: João Paulo L Franco Cairo, Flávia C Leonardo, Thabata M Alvarez, Daniela A Ribeiro, Fernanda Büchli, Ana M Costa-Leonardo, Marcelo F Carazzolle, Fernando F Costa, Adriana F Paes Leme, Gonçalo AG Pereira and Fabio M Squina

    Citation: Biotechnology for Biofuels 2011 4:50

    Content type: Research

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  32. Softwoods are the dominant source of lignocellulosic biomass in the northern hemisphere, and have been investigated worldwide as a renewable substrate for cellulosic ethanol production. One challenge to using ...

    Authors: Gary M Hawkins and Joy Doran-Peterson

    Citation: Biotechnology for Biofuels 2011 4:49

    Content type: Research

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  33. Recently developed iron cocatalyst enhancement of dilute acid pretreatment of biomass is a promising approach for enhancing sugar release from recalcitrant lignocellulosic biomass. However, very little is know...

    Authors: Hui Wei, Bryon S Donohoe, Todd B Vinzant, Peter N Ciesielski, Wei Wang, Lynn M Gedvilas, Yining Zeng, David K Johnson, Shi-You Ding, Michael E Himmel and Melvin P Tucker

    Citation: Biotechnology for Biofuels 2011 4:48

    Content type: Research

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  34. Biodiesel is considered to be a promising future substitute for fossil fuels, and microalgae are one source of biodiesel. The ratios of lipid, carbohydrates and proteins are different in different microalgal s...

    Authors: Cheng Yuan, Junhan Liu, Yong Fan, Xiaohui Ren, Guangrong Hu and Fuli Li

    Citation: Biotechnology for Biofuels 2011 4:47

    Content type: Research

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  35. In the normal process of bioethanol production, biomass is transported to integrated large factories for degradation to sugar, fermentation, and recovery of ethanol by distillation. Biomass nutrient loss occur...

    Authors: Hiroko K Kitamoto, Mitsuo Horita, Yimin Cai, Yukiko Shinozaki and Keiji Sakaki

    Citation: Biotechnology for Biofuels 2011 4:46

    Content type: Research

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  36. Lignin is a highly abundant biopolymer synthesized by plants as a complex component of plant secondary cell walls. Efforts to utilize lignin-based bioproducts are needed.

    Authors: Venugopal Mendu, Anne E Harman-Ware, Mark Crocker, Jungho Jae, Jozsef Stork, Samuel Morton III, Andrew Placido, George Huber and Seth DeBolt

    Citation: Biotechnology for Biofuels 2011 4:43

    Content type: Research

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  37. The enzymatic production of biodiesel through alcoholysis of triglycerides has become more attractive because it shows potential in overcoming the drawbacks of chemical processes. In this study, we investigate...

    Authors: Koei Kawakami, Yasuhiro Oda and Ryo Takahashi

    Citation: Biotechnology for Biofuels 2011 4:42

    Content type: Research

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  38. In converting biomass to bioethanol, pretreatment is a key step intended to render cellulose more amenable and accessible to cellulase enzymes and thus increase glucose yields. In this study, four cellulose sa...

    Authors: Ashutosh Mittal, Rui Katahira, Michael E Himmel and David K Johnson

    Citation: Biotechnology for Biofuels 2011 4:41

    Content type: Research

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  39. Considering that the costs of cellulases and hemicellulases contribute substantially to the price of bioethanol, new studies aimed at understanding and improving cellulase efficiency and productivity are of pa...

    Authors: Wagner R de Souza, Paula F de Gouvea, Marcela Savoldi, Iran Malavazi, Luciano A de Souza Bernardes, Maria Helena S Goldman, Ronald P de Vries, Juliana V de Castro Oliveira and Gustavo H Goldman

    Citation: Biotechnology for Biofuels 2011 4:40

    Content type: Research

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  40. Hydrolysates of plant biomass used for the production of lignocellulosic biofuels typically contain sugar mixtures consisting mainly of D-glucose and D-xylose, and minor amounts of L-arabinose. The yeast Saccharo...

    Authors: Thorsten Subtil and Eckhard Boles

    Citation: Biotechnology for Biofuels 2011 4:38

    Content type: Research

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  41. Irpex lacteus, a versatile lignin-degrading fungus with various extracellular enzymes, has been widely used for biological pretreatment. However, most studies have focused on the change of substrate structure aft...

    Authors: Wanqing Du, Hongbo Yu, Lili Song, Ji Zhang, Changlong Weng, Fuying Ma and Xiaoyu Zhang

    Citation: Biotechnology for Biofuels 2011 4:37

    Content type: Research

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