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  1. Plant biomass is a potentially important renewable source of energy and industrial products. The natural recalcitrance of the cell walls to enzymatic degradation (saccharification), which plants have evolved t...

    Authors: Fedra Francocci, Elisa Bastianelli, Vincenzo Lionetti, Simone Ferrari, Giulia De Lorenzo, Daniela Bellincampi and Felice Cervone
    Citation: Biotechnology for Biofuels 2013 6:163
  2. The rapid determination of the release of structural sugars from biomass feedstocks is an important enabling technology for the development of cellulosic biofuels. An assay that is used to determine sugar rele...

    Authors: Edward J Wolfrum, Ryan M Ness, Nicholas J Nagle, Darren J Peterson and Christopher J Scarlata
    Citation: Biotechnology for Biofuels 2013 6:162
  3. Recently, interest in the utilization of corncob residue (CCR, with high lignin of 45.1%) as a feedstock for bioethanol has been growing. Surfactants have been one of the most popular additives intended to pre...

    Authors: Yue Feng, Jianxin Jiang, Liwei Zhu, Linyan Yue, Junhui Zhang and Shijie Han
    Citation: Biotechnology for Biofuels 2013 6:161
  4. Lignocellulosic bioethanol is expected to play an important role in fossil fuel replacement in the short term. Process integration, improvements in water economy, and increased ethanol titers are key considera...

    Authors: Antonio D Moreno, Elia Tomás-Pejó, David Ibarra, Mercedes Ballesteros and Lisbeth Olsson
    Citation: Biotechnology for Biofuels 2013 6:160
  5. Liquid hot water (LHW) pretreatment is an effective and environmentally friendly method to produce bioethanol with lignocellulosic materials. In our previous study, high ethanol concentration and ethanol yield...

    Authors: Jie Lu, Xuezhi Li, Ruifeng Yang, Jian Zhao and Yinbo Qu
    Citation: Biotechnology for Biofuels 2013 6:159
  6. Sugar beet and intermediates of sugar beet processing are considered to be very attractive feedstock for ethanol production due to their content of fermentable sugars. In particular, the processing of the inte...

    Authors: Piotr Dziugan, Maria Balcerek, Katarzyna Pielech-Przybylska and Piotr Patelski
    Citation: Biotechnology for Biofuels 2013 6:158
  7. Glycerol has attracted attention as a carbon source for microbial production processes due to the large amounts of crude glycerol waste resulting from biodiesel production. The current knowledge about the gene...

    Authors: Steve Swinnen, Mathias Klein, Martina Carrillo, Joseph McInnes, Huyen Thanh Thi Nguyen and Elke Nevoigt
    Citation: Biotechnology for Biofuels 2013 6:157
  8. Thermochemical pretreatment of lignocellulose is crucial to bioconversion in the fields of biorefinery and biofuels. However, the enzyme inhibitors in pretreatment hydrolysate make solid substrate washing and ...

    Authors: Zhaojiang Wang, JY Zhu, Yingjuan Fu, Menghua Qin, Zhiyong Shao, Jungang Jiang and Fang Yang
    Citation: Biotechnology for Biofuels 2013 6:156
  9. 2,3-Butanediol is a platform and fuel biochemical that can be efficiently produced from biomass. However, a value-added process for this chemical has not yet been developed. To expand the utilization of 2,3-bu...

    Authors: Xiuqing Wang, Min Lv, Lijie Zhang, Kun Li, Chao Gao, Cuiqing Ma and Ping Xu
    Citation: Biotechnology for Biofuels 2013 6:155
  10. Ionic liquid (IL) pretreatment is receiving significant attention as a potential process that enables fractionation of lignocellulosic biomass and produces high yields of fermentable sugars suitable for the pr...

    Authors: Chenlin Li, Deepti Tanjore, Wei He, Jessica Wong, James L Gardner, Kenneth L Sale, Blake A Simmons and Seema Singh
    Citation: Biotechnology for Biofuels 2013 6:154
  11. Pretreatment of lignocellulosic biomass is a prerequisite for effective saccharification to produce fermentable sugars. In this study, “green” solvent systems based on acidified mixtures of glycerol carbonate ...

    Authors: Zhanying Zhang, Darryn W Rackemann, William O S Doherty and Ian M O’Hara
    Citation: Biotechnology for Biofuels 2013 6:153
  12. One of the main obstacles for continuous productivity in microalgae cultivation is the presence of biological contaminants capable of eliminating large numbers of cells in a matter of days or even hours. Howev...

    Authors: Leonardo Brantes Bacellar Mendes and Alane Beatriz Vermelho
    Citation: Biotechnology for Biofuels 2013 6:152
  13. Development of industrial yeast strains with high tolerance towards the inhibitors released during biomass pretreatment is critical for bioethanol production. Combining this trait with increased thermotoleranc...

    Authors: Valeria Wallace-Salinas and Marie F Gorwa-Grauslund
    Citation: Biotechnology for Biofuels 2013 6:151
  14. Due to the growing need to provide alternatives to fossil fuels as efficiently, economically, and sustainably as possible there has been growing interest in improved biofuel production systems. Biofuels produc...

    Authors: Sowmya Subramanian, Amanda N Barry, Shayani Pieris and Richard T Sayre
    Citation: Biotechnology for Biofuels 2013 6:150
  15. Aspergillus fumigatus Z5 has a strong ability to decompose lignocellulose biomass, and its extracellular protein secretion has been reported in earlier studies employing traditional techniques. However, a compreh...

    Authors: Dongyang Liu, Juan Li, Shuang Zhao, Ruifu Zhang, Mengmeng Wang, Youzhi Miao, Yifei Shen and Qirong Shen
    Citation: Biotechnology for Biofuels 2013 6:149
  16. The oxidation of carbohydrates from lignocellulose can facilitate the synthesis of new biopolymers and biochemicals, and also reduce sugar metabolism by lignocellulolytic microorganisms, reserving aldonates fo...

    Authors: Thu V Vuong, Arja-Helena Vesterinen, Maryam Foumani, Minna Juvonen, Jukka Seppälä, Maija Tenkanen and Emma R Master
    Citation: Biotechnology for Biofuels 2013 6:148
  17. Enzymatic hydrolysis of lignocellulosic biomass (mainly plant cell walls) is a critical process for biofuel production. This process is greatly hindered by the natural complexity of plant cell walls and limite...

    Authors: Mengmeng Zhang, Guojun Chen, Rajeev Kumar and Bingqian Xu
    Citation: Biotechnology for Biofuels 2013 6:147
  18. Improving saccharification efficiency in bioenergy crop species remains an important challenge. Here, we report the characterization of a Sorghum (Sorghum bicolor L.) mutant, named REDforGREEN (RG), as a bioenerg...

    Authors: Carloalberto Petti, Anne E Harman-Ware, Mizuki Tateno, Rekha Kushwaha, Andrew Shearer, A Bruce Downie, Mark Crocker and Seth DeBolt
    Citation: Biotechnology for Biofuels 2013 6:146
  19. Saccharification and fermentation of pretreated lignocellulosic materials, such as spruce, should be performed at high solids contents in order to reduce the cost of the produced bioethanol. However, this has ...

    Authors: Kerstin Hoyer, Mats Galbe and Guido Zacchi
    Citation: Biotechnology for Biofuels 2013 6:145
  20. Biotechnological exploitation of lignocellulosic biomass is promising for sustainable and environmentally sound energy provision strategy because of the abundant availability of the renewable resources. Wheat ...

    Authors: Saima Shahzad Mirza, Javed Iqbal Qazi, Quanbao Zhao and Shulin Chen
    Citation: Biotechnology for Biofuels 2013 6:144
  21. Biodiesel production from oleaginous microalgae shows great potential as a promising alternative to conventional fossil fuels. Currently, most research focus on algal biomass production with autotrophic cultiv...

    Authors: Hong-Yu Ren, Bing-Feng Liu, Chao Ma, Lei Zhao and Nan-Qi Ren
    Citation: Biotechnology for Biofuels 2013 6:143
  22. Sugarcane is the most efficient crop for production of (1G) ethanol. Additionally, sugarcane bagasse can be used to produce (2G) ethanol. However, the manufacture of 2G ethanol in large scale is not a consolid...

    Authors: Felipe F Furlan, Renato Tonon Filho, Fabio HPB Pinto, Caliane BB Costa, Antonio JG Cruz, Raquel LC Giordano and Roberto C Giordano
    Citation: Biotechnology for Biofuels 2013 6:142
  23. The availability of feedstock options is a key to meeting the volumetric requirement of 136.3 billion liters of renewable fuels per year beginning in 2022, as required in the US 2007 Energy Independence and Se...

    Authors: Hao Cai, Jennifer B Dunn, Zhichao Wang, Jeongwoo Han and Michael Q Wang
    Citation: Biotechnology for Biofuels 2013 6:141
  24. Jatropha curcas L. is an oil seed producing non-leguminous tropical shrub that has good potential to be a fuel plant that can be cultivated on marginal land. Due to the low nutrient content of the targeted planta...

    Authors: Munusamy Madhaiyan, Ni Peng, Ngoh Si Te, Cheng Hsin I, Cai Lin, Fu Lin, Chalapathy Reddy, Hong Yan and Lianghui Ji
    Citation: Biotechnology for Biofuels 2013 6:140
  25. While most resources in biofuels were directed towards implementing bioethanol programs, 1-propanol has recently received attention as a promising alternative biofuel. Nevertheless, no microorganism has been i...

    Authors: Kajan Srirangan, Lamees Akawi, Xuejia Liu, Adam Westbrook, Eric JM Blondeel, Marc G Aucoin, Murray Moo-Young and C Perry Chou
    Citation: Biotechnology for Biofuels 2013 6:139
  26. Butanol (n-butanol) has high values as a promising fuel source and chemical feedstock. Biobutanol is usually produced by the solventogenic clostridia through a typical biphasic (acidogenesis and solventogenesi...

    Authors: Yi Wang, Xiangzhen Li and Hans P Blaschek
    Citation: Biotechnology for Biofuels 2013 6:138
  27. Microbial production of biofuels requires robust cell growth and metabolism under tough conditions. Conventionally, such tolerance phenotypes were engineered through evolutionary engineering using the principl...

    Authors: Guodong Luan, Zhen Cai, Yin Li and Yanhe Ma
    Citation: Biotechnology for Biofuels 2013 6:137
  28. Obtaining bioethanol from cellulosic biomass involves numerous steps, among which the enzymatic conversion of the polymer to individual sugar units has been a main focus of the biotechnology industry. Among th...

    Authors: Ragothaman M Yennamalli, Andrew J Rader, Adam J Kenny, Jeffrey D Wolt and Taner Z Sen
    Citation: Biotechnology for Biofuels 2013 6:136
  29. There has been a great deal of interest in fuel productions from lignocellulosic biomass to minimize the conflict between food and fuel use. The bioconversion of xylose, which is the second most abundant sugar...

    Authors: Kazuhiko Kurosawa, Sandra J Wewetzer and Anthony J Sinskey
    Citation: Biotechnology for Biofuels 2013 6:134
  30. VHG fermentation is a promising process engineering strategy aiming at improving ethanol titer, and thus saving energy consumption for ethanol distillation and distillage treatment. However, sustained process ...

    Authors: Liang Wang, Xin-Qing Zhao, Chuang Xue and Feng-Wu Bai
    Citation: Biotechnology for Biofuels 2013 6:133
  31. The thermophilic anaerobe Clostridium thermocellum is a candidate consolidated bioprocessing (CBP) biocatalyst for cellulosic ethanol production. It is capable of both cellulose solubilization and its fermentatio...

    Authors: Charlotte M Wilson, Shihui Yang, Miguel Rodriguez Jr, Qin Ma, Courtney M Johnson, Lezlee Dice, Ying Xu and Steven D Brown
    Citation: Biotechnology for Biofuels 2013 6:131
  32. Though n-butanol has been proposed as a potential transportation biofuel, its toxicity often causes oxidative stress in the host microorganism and is considered one of the bottlenecks preventing its efficient mas...

    Authors: Wei-Chih Chin, Kuo-Hsing Lin, Jui-Jen Chang and Chieh-Chen Huang
    Citation: Biotechnology for Biofuels 2013 6:130
  33. Recently, Jatropha curcas L. has attracted worldwide attention for its potential as a source of biodiesel. However, most DNA markers have demonstrated high levels of genetic similarity among and within jatropha p...

    Authors: Atefeh Alipour, Suguru Tsuchimoto, Hiroe Sakai, Nobuko Ohmido and Kiichi Fukui
    Citation: Biotechnology for Biofuels 2013 6:129
  34. Renewable lignocellulosic biomass is an advantageous resource for the production of second generation biofuels and other biorefinery products. In Middle Europe, wheat straw is one of the most abundant low-cost...

    Authors: Robert Bischof, Lukas Fourtis, Andreas Limbeck, Christian Gamauf, Bernhard Seiboth and Christian P Kubicek
    Citation: Biotechnology for Biofuels 2013 6:127
  35. Complete hydrolysis of cellulose to glucose requires the synergistic action of three general types of glycoside hydrolases; endoglucanases, exoglucanases, and cellobiases. Cellulases that are found in Nature v...

    Authors: Qi Xu, Shi-You Ding, Roman Brunecky, Yannick J Bomble, Michael E Himmel and John O Baker
    Citation: Biotechnology for Biofuels 2013 6:126
  36. Redox-cofactor balancing constrains product yields in anaerobic fermentation processes. This challenge is exemplified by the formation of glycerol as major by-product in yeast-based bioethanol production, whic...

    Authors: Víctor Guadalupe-Medina, H Wouter Wisselink, Marijke AH Luttik, Erik de Hulster, Jean-Marc Daran, Jack T Pronk and Antonius JA van Maris
    Citation: Biotechnology for Biofuels 2013 6:125
  37. Efficient conversion of lignocellulosic biomass to optically pure lactic acid is a key challenge for the economical production of biodegradable poly-lactic acid. A recently isolated strain, Thermoanaerobacterium ...

    Authors: Xiaofeng Yang, Zhicheng Lai, Chaofeng Lai, Muzi Zhu, Shuang Li, Jufang Wang and Xiaoning Wang
    Citation: Biotechnology for Biofuels 2013 6:124
  38. 2,3-Butanediol (2,3-BD), a platform and fuel bio-chemical, can be efficiently produced by Klebsiella pneumonia, K. oxytoca, and Serratia marcescens. However, these strains are opportunistic pathogens and not favo...

    Authors: Lixiang Li, Lijie Zhang, Kun Li, Yu Wang, Chao Gao, Binbin Han, Cuiqing Ma and Ping Xu
    Citation: Biotechnology for Biofuels 2013 6:123
  39. Fermentative hydrogen production is an attractive means for the sustainable production of this future energy carrier but is hampered by low yields. One possible solution is to create, using metabolic engineeri...

    Authors: Dipankar Ghosh, Ariane Bisaillon and Patrick C Hallenbeck
    Citation: Biotechnology for Biofuels 2013 6:122
  40. In addition to efficient pentose utilization, high inhibitor tolerance is a key trait required in any organism used for economically viable industrial bioethanol production with lignocellulose biomass. Althoug...

    Authors: Mekonnen M Demeke, Françoise Dumortier, Yingying Li, Tom Broeckx, María R Foulquié-Moreno and Johan M Thevelein
    Citation: Biotechnology for Biofuels 2013 6:120
  41. One route for producing cellulosic biofuels is by the fermentation of lignocellulose-derived sugars generated from a pretreatment that can be effectively coupled with an enzymatic hydrolysis of the plant cell ...

    Authors: Zhenglun Li, Charles H Chen, Eric L Hegg and David B Hodge
    Citation: Biotechnology for Biofuels 2013 6:119
  42. Microalgae can make a significant contribution towards meeting global renewable energy needs in both carbon-based and hydrogen (H2) biofuel. The development of energy-related products from algae could be accelera...

    Authors: Shihui Yang, Michael T Guarnieri, Sharon Smolinski, Maria Ghirardi and Philip T Pienkos
    Citation: Biotechnology for Biofuels 2013 6:118
  43. Consolidated bioprocessing (CBP) is reliant on the simultaneous enzyme production, saccharification of biomass, and fermentation of released sugars into valuable products such as butanol. Clostridial species t...

    Authors: Katalin Kovács, Benjamin J Willson, Katrin Schwarz, John T Heap, Adam Jackson, David N Bolam, Klaus Winzer and Nigel P Minton
    Citation: Biotechnology for Biofuels 2013 6:117
  44. Ensiling is a well-known method for preserving green biomasses through anaerobic production of organic acids by lactic acid bacteria. In this study, wheat straw is subjected to ensiling in combination with hyd...

    Authors: Morten Ambye-Jensen, Sune Tjalfe Thomsen, Zsófia Kádár and Anne S Meyer
    Citation: Biotechnology for Biofuels 2013 6:116
  45. Identifying new high-performance enzymes or enzyme complexes to enhance biomass degradation is the key for the development of cost-effective processes for ethanol production. Irpex lacteus is an efficient microor...

    Authors: Davinia Salvachúa, Angel T Martínez, Ming Tien, María F López-Lucendo, Francisco García, Vivian de los Ríos, María Jesús Martínez and Alicia Prieto
    Citation: Biotechnology for Biofuels 2013 6:115
  46. Little is known about the levels of variation in lignin or other wood related genes in Salix, a genus that is being increasingly used for biomass and biofuel production. The lignin biosynthesis pathway is well ch...

    Authors: Aude C Perdereau, Gerry C Douglas, Trevor R Hodkinson and Colin T Kelleher
    Citation: Biotechnology for Biofuels 2013 6:114