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  1. Microbial cellulose conversion by Clostridium thermocellum 27405 occurs predominantly through the activity of substrate-adherent bacteria organized in thin, primarily single cell-layered biofilms. The importance ...

    Authors: Alexandru Dumitrache, Gideon M Wolfaardt, David Grant Allen, Steven N Liss and Lee R Lynd
    Citation: Biotechnology for Biofuels 2013 6:175
  2. Sweet sorghum is regarded as a very promising energy crop for ethanol production because it not only supplies grain and sugar, but also offers lignocellulosic resource. Cost-competitive ethanol production requ...

    Authors: Jihong Li, Shizhong Li, Bing Han, Menghui Yu, Guangming Li and Yan Jiang
    Citation: Biotechnology for Biofuels 2013 6:174
  3. Bamboo is potentially an interesting feedstock for advanced bioethanol production in China due to its natural abundance, rapid growth, perennial nature and low management requirements. Liquid hot water (LHW) p...

    Authors: Jade Littlewood, Lei Wang, Colin Turnbull and Richard J Murphy
    Citation: Biotechnology for Biofuels 2013 6:173
  4. The lignocellulosic enzymes of Trichoderma species have received particular attention with regard to biomass conversion to biofuels, but the production cost of these enzymes remains a significant hurdle for their...

    Authors: Isa Jacoba Marx, Niël van Wyk, Salome Smit, Daniel Jacobson, Marinda Viljoen-Bloom and Heinrich Volschenk
    Citation: Biotechnology for Biofuels 2013 6:172
  5. Empty fruit bunch (EFB) has many advantages, including its abundance, the fact that it does not require collection, and its year-round availability as a feedstock for bioethanol production. But before the sign...

    Authors: Won-Il Choi, Ji-Yeon Park, Joon-Pyo Lee, You-Kwan Oh, Yong Chul Park, Jun Seok Kim, Jang Min Park, Chul Ho Kim and Jin-Suk Lee
    Citation: Biotechnology for Biofuels 2013 6:170
  6. Integration of second-generation (2G) bioethanol production with existing first-generation (1G) production may facilitate commercial production of ethanol from cellulosic material. Since 2G hydrolysates have a...

    Authors: Borbála Erdei, Dóra Hancz, Mats Galbe and Guido Zacchi
    Citation: Biotechnology for Biofuels 2013 6:169
  7. Robust yeasts with high inhibitor, temperature, and osmotic tolerance remain a crucial requirement for the sustainable production of lignocellulosic bioethanol. These stress factors are known to severely hinde...

    Authors: Lorenzo Favaro, Marina Basaglia, Alberto Trento, Eugéne Van Rensburg, Maria García-Aparicio, Willem H Van Zyl and Sergio Casella
    Citation: Biotechnology for Biofuels 2013 6:168
  8. Starch is one of the most abundant organic polysaccharides available for the production of bio-ethanol as an alternative transport fuel. Cost-effective utilisation of starch requires consolidated bioprocessing...

    Authors: Marko J Viktor, Shaunita H Rose, Willem H van Zyl and Marinda Viljoen-Bloom
    Citation: Biotechnology for Biofuels 2013 6:167
  9. In solid-state anaerobic digestion (AD) bioprocesses, hydrolytic and acidogenic microbial metabolisms have not yet been clarified. Since these stages are particularly important for the establishment of the bio...

    Authors: Jean-Charles Motte, Eric Trably, Renaud Escudié, Jérôme Hamelin, Jean-Philippe Steyer, Nicolas Bernet, Jean-Philippe Delgenes and Claire Dumas
    Citation: Biotechnology for Biofuels 2013 6:164
  10. Plant biomass is a potentially important renewable source of energy and industrial products. The natural recalcitrance of the cell walls to enzymatic degradation (saccharification), which plants have evolved t...

    Authors: Fedra Francocci, Elisa Bastianelli, Vincenzo Lionetti, Simone Ferrari, Giulia De Lorenzo, Daniela Bellincampi and Felice Cervone
    Citation: Biotechnology for Biofuels 2013 6:163
  11. The rapid determination of the release of structural sugars from biomass feedstocks is an important enabling technology for the development of cellulosic biofuels. An assay that is used to determine sugar rele...

    Authors: Edward J Wolfrum, Ryan M Ness, Nicholas J Nagle, Darren J Peterson and Christopher J Scarlata
    Citation: Biotechnology for Biofuels 2013 6:162
  12. Recently, interest in the utilization of corncob residue (CCR, with high lignin of 45.1%) as a feedstock for bioethanol has been growing. Surfactants have been one of the most popular additives intended to pre...

    Authors: Yue Feng, Jianxin Jiang, Liwei Zhu, Linyan Yue, Junhui Zhang and Shijie Han
    Citation: Biotechnology for Biofuels 2013 6:161
  13. Lignocellulosic bioethanol is expected to play an important role in fossil fuel replacement in the short term. Process integration, improvements in water economy, and increased ethanol titers are key considera...

    Authors: Antonio D Moreno, Elia Tomás-Pejó, David Ibarra, Mercedes Ballesteros and Lisbeth Olsson
    Citation: Biotechnology for Biofuels 2013 6:160
  14. Liquid hot water (LHW) pretreatment is an effective and environmentally friendly method to produce bioethanol with lignocellulosic materials. In our previous study, high ethanol concentration and ethanol yield...

    Authors: Jie Lu, Xuezhi Li, Ruifeng Yang, Jian Zhao and Yinbo Qu
    Citation: Biotechnology for Biofuels 2013 6:159
  15. Sugar beet and intermediates of sugar beet processing are considered to be very attractive feedstock for ethanol production due to their content of fermentable sugars. In particular, the processing of the inte...

    Authors: Piotr Dziugan, Maria Balcerek, Katarzyna Pielech-Przybylska and Piotr Patelski
    Citation: Biotechnology for Biofuels 2013 6:158
  16. Glycerol has attracted attention as a carbon source for microbial production processes due to the large amounts of crude glycerol waste resulting from biodiesel production. The current knowledge about the gene...

    Authors: Steve Swinnen, Mathias Klein, Martina Carrillo, Joseph McInnes, Huyen Thanh Thi Nguyen and Elke Nevoigt
    Citation: Biotechnology for Biofuels 2013 6:157
  17. Thermochemical pretreatment of lignocellulose is crucial to bioconversion in the fields of biorefinery and biofuels. However, the enzyme inhibitors in pretreatment hydrolysate make solid substrate washing and ...

    Authors: Zhaojiang Wang, JY Zhu, Yingjuan Fu, Menghua Qin, Zhiyong Shao, Jungang Jiang and Fang Yang
    Citation: Biotechnology for Biofuels 2013 6:156
  18. 2,3-Butanediol is a platform and fuel biochemical that can be efficiently produced from biomass. However, a value-added process for this chemical has not yet been developed. To expand the utilization of 2,3-bu...

    Authors: Xiuqing Wang, Min Lv, Lijie Zhang, Kun Li, Chao Gao, Cuiqing Ma and Ping Xu
    Citation: Biotechnology for Biofuels 2013 6:155
  19. Ionic liquid (IL) pretreatment is receiving significant attention as a potential process that enables fractionation of lignocellulosic biomass and produces high yields of fermentable sugars suitable for the pr...

    Authors: Chenlin Li, Deepti Tanjore, Wei He, Jessica Wong, James L Gardner, Kenneth L Sale, Blake A Simmons and Seema Singh
    Citation: Biotechnology for Biofuels 2013 6:154
  20. Pretreatment of lignocellulosic biomass is a prerequisite for effective saccharification to produce fermentable sugars. In this study, “green” solvent systems based on acidified mixtures of glycerol carbonate ...

    Authors: Zhanying Zhang, Darryn W Rackemann, William O S Doherty and Ian M O’Hara
    Citation: Biotechnology for Biofuels 2013 6:153
  21. One of the main obstacles for continuous productivity in microalgae cultivation is the presence of biological contaminants capable of eliminating large numbers of cells in a matter of days or even hours. Howev...

    Authors: Leonardo Brantes Bacellar Mendes and Alane Beatriz Vermelho
    Citation: Biotechnology for Biofuels 2013 6:152
  22. Development of industrial yeast strains with high tolerance towards the inhibitors released during biomass pretreatment is critical for bioethanol production. Combining this trait with increased thermotoleranc...

    Authors: Valeria Wallace-Salinas and Marie F Gorwa-Grauslund
    Citation: Biotechnology for Biofuels 2013 6:151
  23. Due to the growing need to provide alternatives to fossil fuels as efficiently, economically, and sustainably as possible there has been growing interest in improved biofuel production systems. Biofuels produc...

    Authors: Sowmya Subramanian, Amanda N Barry, Shayani Pieris and Richard T Sayre
    Citation: Biotechnology for Biofuels 2013 6:150
  24. Aspergillus fumigatus Z5 has a strong ability to decompose lignocellulose biomass, and its extracellular protein secretion has been reported in earlier studies employing traditional techniques. However, a compreh...

    Authors: Dongyang Liu, Juan Li, Shuang Zhao, Ruifu Zhang, Mengmeng Wang, Youzhi Miao, Yifei Shen and Qirong Shen
    Citation: Biotechnology for Biofuels 2013 6:149
  25. The oxidation of carbohydrates from lignocellulose can facilitate the synthesis of new biopolymers and biochemicals, and also reduce sugar metabolism by lignocellulolytic microorganisms, reserving aldonates fo...

    Authors: Thu V Vuong, Arja-Helena Vesterinen, Maryam Foumani, Minna Juvonen, Jukka Seppälä, Maija Tenkanen and Emma R Master
    Citation: Biotechnology for Biofuels 2013 6:148
  26. Enzymatic hydrolysis of lignocellulosic biomass (mainly plant cell walls) is a critical process for biofuel production. This process is greatly hindered by the natural complexity of plant cell walls and limite...

    Authors: Mengmeng Zhang, Guojun Chen, Rajeev Kumar and Bingqian Xu
    Citation: Biotechnology for Biofuels 2013 6:147
  27. Improving saccharification efficiency in bioenergy crop species remains an important challenge. Here, we report the characterization of a Sorghum (Sorghum bicolor L.) mutant, named REDforGREEN (RG), as a bioenerg...

    Authors: Carloalberto Petti, Anne E Harman-Ware, Mizuki Tateno, Rekha Kushwaha, Andrew Shearer, A Bruce Downie, Mark Crocker and Seth DeBolt
    Citation: Biotechnology for Biofuels 2013 6:146
  28. Saccharification and fermentation of pretreated lignocellulosic materials, such as spruce, should be performed at high solids contents in order to reduce the cost of the produced bioethanol. However, this has ...

    Authors: Kerstin Hoyer, Mats Galbe and Guido Zacchi
    Citation: Biotechnology for Biofuels 2013 6:145
  29. Biotechnological exploitation of lignocellulosic biomass is promising for sustainable and environmentally sound energy provision strategy because of the abundant availability of the renewable resources. Wheat ...

    Authors: Saima Shahzad Mirza, Javed Iqbal Qazi, Quanbao Zhao and Shulin Chen
    Citation: Biotechnology for Biofuels 2013 6:144
  30. Biodiesel production from oleaginous microalgae shows great potential as a promising alternative to conventional fossil fuels. Currently, most research focus on algal biomass production with autotrophic cultiv...

    Authors: Hong-Yu Ren, Bing-Feng Liu, Chao Ma, Lei Zhao and Nan-Qi Ren
    Citation: Biotechnology for Biofuels 2013 6:143
  31. Sugarcane is the most efficient crop for production of (1G) ethanol. Additionally, sugarcane bagasse can be used to produce (2G) ethanol. However, the manufacture of 2G ethanol in large scale is not a consolid...

    Authors: Felipe F Furlan, Renato Tonon Filho, Fabio HPB Pinto, Caliane BB Costa, Antonio JG Cruz, Raquel LC Giordano and Roberto C Giordano
    Citation: Biotechnology for Biofuels 2013 6:142
  32. The availability of feedstock options is a key to meeting the volumetric requirement of 136.3 billion liters of renewable fuels per year beginning in 2022, as required in the US 2007 Energy Independence and Se...

    Authors: Hao Cai, Jennifer B Dunn, Zhichao Wang, Jeongwoo Han and Michael Q Wang
    Citation: Biotechnology for Biofuels 2013 6:141
  33. Jatropha curcas L. is an oil seed producing non-leguminous tropical shrub that has good potential to be a fuel plant that can be cultivated on marginal land. Due to the low nutrient content of the targeted planta...

    Authors: Munusamy Madhaiyan, Ni Peng, Ngoh Si Te, Cheng Hsin I, Cai Lin, Fu Lin, Chalapathy Reddy, Hong Yan and Lianghui Ji
    Citation: Biotechnology for Biofuels 2013 6:140
  34. While most resources in biofuels were directed towards implementing bioethanol programs, 1-propanol has recently received attention as a promising alternative biofuel. Nevertheless, no microorganism has been i...

    Authors: Kajan Srirangan, Lamees Akawi, Xuejia Liu, Adam Westbrook, Eric JM Blondeel, Marc G Aucoin, Murray Moo-Young and C Perry Chou
    Citation: Biotechnology for Biofuels 2013 6:139
  35. Butanol (n-butanol) has high values as a promising fuel source and chemical feedstock. Biobutanol is usually produced by the solventogenic clostridia through a typical biphasic (acidogenesis and solventogenesi...

    Authors: Yi Wang, Xiangzhen Li and Hans P Blaschek
    Citation: Biotechnology for Biofuels 2013 6:138
  36. Microbial production of biofuels requires robust cell growth and metabolism under tough conditions. Conventionally, such tolerance phenotypes were engineered through evolutionary engineering using the principl...

    Authors: Guodong Luan, Zhen Cai, Yin Li and Yanhe Ma
    Citation: Biotechnology for Biofuels 2013 6:137
  37. Obtaining bioethanol from cellulosic biomass involves numerous steps, among which the enzymatic conversion of the polymer to individual sugar units has been a main focus of the biotechnology industry. Among th...

    Authors: Ragothaman M Yennamalli, Andrew J Rader, Adam J Kenny, Jeffrey D Wolt and Taner Z Sen
    Citation: Biotechnology for Biofuels 2013 6:136
  38. There has been a great deal of interest in fuel productions from lignocellulosic biomass to minimize the conflict between food and fuel use. The bioconversion of xylose, which is the second most abundant sugar...

    Authors: Kazuhiko Kurosawa, Sandra J Wewetzer and Anthony J Sinskey
    Citation: Biotechnology for Biofuels 2013 6:134
  39. VHG fermentation is a promising process engineering strategy aiming at improving ethanol titer, and thus saving energy consumption for ethanol distillation and distillage treatment. However, sustained process ...

    Authors: Liang Wang, Xin-Qing Zhao, Chuang Xue and Feng-Wu Bai
    Citation: Biotechnology for Biofuels 2013 6:133
  40. The thermophilic anaerobe Clostridium thermocellum is a candidate consolidated bioprocessing (CBP) biocatalyst for cellulosic ethanol production. It is capable of both cellulose solubilization and its fermentatio...

    Authors: Charlotte M Wilson, Shihui Yang, Miguel Rodriguez Jr, Qin Ma, Courtney M Johnson, Lezlee Dice, Ying Xu and Steven D Brown
    Citation: Biotechnology for Biofuels 2013 6:131
  41. Though n-butanol has been proposed as a potential transportation biofuel, its toxicity often causes oxidative stress in the host microorganism and is considered one of the bottlenecks preventing its efficient mas...

    Authors: Wei-Chih Chin, Kuo-Hsing Lin, Jui-Jen Chang and Chieh-Chen Huang
    Citation: Biotechnology for Biofuels 2013 6:130
  42. Recently, Jatropha curcas L. has attracted worldwide attention for its potential as a source of biodiesel. However, most DNA markers have demonstrated high levels of genetic similarity among and within jatropha p...

    Authors: Atefeh Alipour, Suguru Tsuchimoto, Hiroe Sakai, Nobuko Ohmido and Kiichi Fukui
    Citation: Biotechnology for Biofuels 2013 6:129
  43. Renewable lignocellulosic biomass is an advantageous resource for the production of second generation biofuels and other biorefinery products. In Middle Europe, wheat straw is one of the most abundant low-cost...

    Authors: Robert Bischof, Lukas Fourtis, Andreas Limbeck, Christian Gamauf, Bernhard Seiboth and Christian P Kubicek
    Citation: Biotechnology for Biofuels 2013 6:127
  44. Complete hydrolysis of cellulose to glucose requires the synergistic action of three general types of glycoside hydrolases; endoglucanases, exoglucanases, and cellobiases. Cellulases that are found in Nature v...

    Authors: Qi Xu, Shi-You Ding, Roman Brunecky, Yannick J Bomble, Michael E Himmel and John O Baker
    Citation: Biotechnology for Biofuels 2013 6:126
  45. Redox-cofactor balancing constrains product yields in anaerobic fermentation processes. This challenge is exemplified by the formation of glycerol as major by-product in yeast-based bioethanol production, whic...

    Authors: Víctor Guadalupe-Medina, H Wouter Wisselink, Marijke AH Luttik, Erik de Hulster, Jean-Marc Daran, Jack T Pronk and Antonius JA van Maris
    Citation: Biotechnology for Biofuels 2013 6:125