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  1. On-site enzyme production using Trichoderma reesei can improve yields and lower the overall cost of lignocellulose saccharification by exploiting the fungal gene regulatory mechanism that enables it to continuous...

    Authors: Vera Novy, Fredrik Nielsen, Daniel Cullen, Grzegorz Sabat, Carl J. Houtman and Christopher G. Hunt

    Citation: Biotechnology for Biofuels 2021 14:105

    Content type: Research

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  2. Understanding how the digestibility of lignocellulosic biomass is affected by its morphology is essential to design efficient processes for biomass deconstruction. In this study, we used a model based on a set...

    Authors: Jessica C. Rohrbach and Jeremy S. Luterbacher

    Citation: Biotechnology for Biofuels 2021 14:103

    Content type: Research

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  3. Plant biomass is a highly abundant renewable resource that can be converted into several types of high-value-added products, including chemicals, biofuels and advanced materials. In the last few decades, an in...

    Authors: Peng Ning, Guofeng Yang, Lihong Hu, Jingxin Sun, Lina Shi, Yonghong Zhou, Zhaobao Wang and Jianming Yang

    Citation: Biotechnology for Biofuels 2021 14:102

    Content type: Review

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  4. Mitigation of climate change requires that new routes for the production of fuels and chemicals be as oil-independent as possible. The microbial conversion of lignocellulosic feedstocks into terpene-based biof...

    Authors: James Kirby, Gina M. Geiselman, Junko Yaegashi, Joonhoon Kim, Xun Zhuang, Mary Bao Tran-Gyamfi, Jan-Philip Prahl, Eric R. Sundstrom, Yuqian Gao, Nathalie Munoz, Kristin E. Burnum-Johnson, Veronica T. Benites, Edward E. K. Baidoo, Anna Fuhrmann, Katharina Seibel, Bobbie-Jo M. Webb-Robertson…

    Citation: Biotechnology for Biofuels 2021 14:101

    Content type: Research

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  5. 4-Hydroxyphenylacetic acid (4HPAA) is an important raw material for the synthesis of drugs, pesticides and biochemicals. Microbial biotechnology would be an attractive approach for 4HPAA production, and cofact...

    Authors: Yu-Ping Shen, Yu-Ling Liao, Qian Lu, Xin He, Zhi-Bo Yan and Jian-Zhong Liu

    Citation: Biotechnology for Biofuels 2021 14:100

    Content type: Research

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  6. Duckweed is considered a promising feedstock for bioethanol production due to its high biomass and starch production. The starch content can be promoted by plant growth regulators after the vegetative reproduc...

    Authors: Yerong Zhu, Xiaoxue Li, Xuan Gao, Jiqi Sun, Xiaoyuan Ji, Guodong Feng, Guangshuang Shen, Beibei Xiang and Yong Wang

    Citation: Biotechnology for Biofuels 2021 14:99

    Content type: Research

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  7. Miscanthus is a commercial lignocellulosic biomass crop owing to its high biomass productivity and low chemical input requirements. Within an interspecific Miscanthus cross, progeny with high biomass yield were s...

    Authors: Jose J. De Vega, Ned Peel, Sarah J. Purdy, Sarah Hawkins, Lain Donnison, Sarah Dyer and Kerrie Farrar

    Citation: Biotechnology for Biofuels 2021 14:98

    Content type: Research

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  8. Xylo-oligosaccharide is the spotlight of functional sugar that improves the economic benefits of lignocellulose biorefinery. Acetic acid acidolysis technology provides a promising application for xylo-oligosac...

    Authors: Jianming Guo, Kaixuan Huang, Rou Cao, Junhua Zhang and Yong Xu

    Citation: Biotechnology for Biofuels 2021 14:97

    Content type: Research

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  9. Wood-decay basidiomycetes are effective for the degradation of highly lignified and recalcitrant plant substrates. The degradation of lignocellulosic materials by brown-rot strains is carried out by carbohydra...

    Authors: Fernanda Lopes de Figueiredo, Ana Carolina Piva de Oliveira, Cesar Rafael Fanchini Terrasan, Thiago Augusto Gonçalves, Jaqueline Aline Gerhardt, Geizecler Tomazetto, Gabriela Felix Persinoti, Marcelo Ventura Rubio, Jennifer Andrea Tamayo Peña, Michelle Fernandes Araújo, Maria Augusta de Carvalho Silvello, Telma Teixeira Franco, Sarita Cândida Rabelo, Rosana Goldbeck, Fabio Marcio Squina and André Damasio

    Citation: Biotechnology for Biofuels 2021 14:96

    Content type: Research

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  10. Rhodosporidium strain, a well-known oleaginous yeast, has been widely used as a platform for lipid and carotenoid production. However, the production of squalene for application in lipid-based biofuels is not rep...

    Authors: Shahryar Shakeri, Farshad Khoshbasirat and Mahmood Maleki

    Citation: Biotechnology for Biofuels 2021 14:95

    Content type: Research

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  11. Seed germination is the most important stage for the formation of a new plant. This process starts when the dry seed begins to absorb water and ends when the radicle protrudes. The germination rate of seed fro...

    Authors: Bingchao Wu, Min Sun, Huan Zhang, Dan Yang, Chuang Lin, Imran Khan, Xiaoshan Wang, Xinquan Zhang, Gang Nie, Guangyan Feng, Yanhong Yan, Zhou Li, Yan Peng and Linkai Huang

    Citation: Biotechnology for Biofuels 2021 14:94

    Content type: Research

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  12. Wild-type yeasts have been successfully used to obtain food products, yet their full potential as fermenting microorganisms for large-scale ethanol fuel production has to be determined. In this study, wild-typ...

    Authors: Enrique Romero-Frasca, Sharon B. Velasquez-Orta, Viviana Escobar-Sánchez, Raunel Tinoco-Valencia and María Teresa Orta Ledesma

    Citation: Biotechnology for Biofuels 2021 14:93

    Content type: Research

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  13. Presence of inhibitory chemicals in lignocellulose hydrolysates is a major hurdle for production of second-generation bioethanol. Especially cheaper pre-treatment methods that ensure an economical viable produ...

    Authors: Gert Vanmarcke, Mekonnen M. Demeke, Maria R. Foulquié-Moreno and Johan M. Thevelein

    Citation: Biotechnology for Biofuels 2021 14:92

    Content type: Research

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  14. Caldicellulosiruptor kronotskyensis has gained interest for its ability to grow on various lignocellulosic biomass. The aim of this study was to investigate the growth profiles of C. kronotskyensis in the presenc...

    Authors: Thitiwut Vongkampang, Krishnan Sreenivas, Jonathan Engvall, Carl Grey and Ed W. J. van Niel

    Citation: Biotechnology for Biofuels 2021 14:91

    Content type: Research

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  15. Burning fast-growing trees for energy production can be an effective alternative to coal combustion. Thus, lignocellulosic material, which can be used to obtain chemicals with a high added value, is highly abu...

    Authors: Alberto Palma, Javier Mauricio Loaiza, Manuel J. Díaz, Juan Carlos García, Inmaculada Giráldez and Francisco López

    Citation: Biotechnology for Biofuels 2021 14:89

    Content type: Research

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  16. Cocksfoot grass (Dactylis glomerata L.) with high biomass yield and rich cellulose can be used to produce bioethanol as fuel additive. In view of this, ultrasonic and hydrothermal pretreatments followed by succes...

    Authors: Shao-Chao Sun, Dan Sun and Xue-Fei Cao

    Citation: Biotechnology for Biofuels 2021 14:88

    Content type: Research

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  17. Global issues such as environmental problems and food security are currently of concern to all of us. Circular bioeconomy is a promising approach towards resolving these global issues. The production of bioene...

    Authors: Hui Yi Leong, Chih-Kai Chang, Kuan Shiong Khoo, Kit Wayne Chew, Shir Reen Chia, Jun Wei Lim, Jo-Shu Chang and Pau Loke Show

    Citation: Biotechnology for Biofuels 2021 14:87

    Content type: Review

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  18. Bioethanol from abundant and inexpensive agricultural and industrial wastes possesses the potential to reduce greenhouse gas emissions. Bioethanol as renewable fuel addresses elevated production costs, as well...

    Authors: Anita Ejiro Nwaefuna, Karl Rumbold, Teun Boekhout and Nerve Zhou

    Citation: Biotechnology for Biofuels 2021 14:86

    Content type: Review

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  19. Wheat straw, one of the most abundant agricultural residues worldwide, can be used to produce biogas, which is considered one of the most efficiently produced renewable energies. Wheat grown with the dual-purp...

    Authors: Nadia Gabbanelli, Elisa Erbetta, Maria Eugenia Sanz Smachetti, Máximo Lorenzo, Paola Mónica Talia, Ignacio Ramírez, Magdalena Vera, Ignacio Durruty, Ana Clara Pontaroli and Maria Mercedes Echarte

    Citation: Biotechnology for Biofuels 2021 14:85

    Content type: Research

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  20. Laccases and laccase-like multicopper oxidases (LMCOs) oxidize a vast array of phenolic compounds and amines, releasing water as a byproduct. Their low substrate specificity is responsible for their tremendous...

    Authors: A. Zerva, C. Pentari, A. Termentzi, A. H. P. America, D. Zouraris, S. K. Bhattacharya, A. Karantonis, G. I. Zervakis and E. Topakas

    Citation: Biotechnology for Biofuels 2021 14:83

    Content type: Research

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  21. The unicellular alga Haematococcus pluvialis has achieved considerable interests for its capacity to accumulate large amounts of triacylglycerol and astaxanthin under various environmental stresses. To our knowle...

    Authors: Qunju Hu, Danqiong Huang, Anguo Li, Zhangli Hu, Zhengquan Gao, Yongli Yang and Chaogang Wang

    Citation: Biotechnology for Biofuels 2021 14:82

    Content type: Research

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  22. Most of the organic content of waste activated sludge (WAS) comprises microbial cells hard to degrade, which must be pre-treated for energy recovery by anaerobic digestion (AD). Electrooxidation pre-treatment ...

    Authors: J. A. Barrios, A. Cano, F. F. Rivera, M. E. Cisneros and U. Durán

    Citation: Biotechnology for Biofuels 2021 14:81

    Content type: Research

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  23. Phytases are widely used commercially as dietary supplements for swine and poultry to increase the digestibility of phytic acid. Enzyme development has focused on increasing thermostability to withstand the hi...

    Authors: Laura Navone, Thomas Vogl, Pawarisa Luangthongkam, Jo-Anne Blinco, Carlos H. Luna-Flores, Xiaojing Chen, Juhani von Hellens, Stephen Mahler and Robert Speight

    Citation: Biotechnology for Biofuels 2021 14:80

    Content type: Research

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  24. Products made from recycled organic materials are an important part of a circular economy, but the question is whether they will be adopted by the public. Such products can elicit strong emotional responses an...

    Authors: Madeline Judge, Olivia de Hoog, Goda Perlaviciute, Nadja Contzen and Linda Steg

    Citation: Biotechnology for Biofuels 2021 14:79

    Content type: Research

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  25. Plant cell wall-derived biomass serves as a renewable source of energy and materials with increasing importance. The cell walls are biomacromolecular assemblies defined by a fine arrangement of different class...

    Authors: Brian T DeVree, Lisa M Steiner, Sylwia Głazowska, Felix Ruhnow, Klaus Herburger, Staffan Persson and Jozef Mravec

    Citation: Biotechnology for Biofuels 2021 14:78

    Content type: Review

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  26. Knowledge with respect to regulatory systems for cellulase production is prerequisite for exploitation of such regulatory networks to increase cellulase production, improve fermentation efficiency and reduce t...

    Authors: Ai-Ping Pang, Haiyan Wang, Funing Zhang, Xin Hu, Fu-Gen Wu, Zhihua Zhou, Wei Wang, Zuhong Lu and Fengming Lin

    Citation: Biotechnology for Biofuels 2021 14:77

    Content type: Research

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  27. Engineering triacylglycerol (TAG) accumulation in vegetative tissues of non-food crops has become a promising way to meet our increasing demand for plant oils, especially the renewable production of biofuels. ...

    Authors: Yu Gao, Yan Sun, Huiling Gao, Ying Chen, Xiaoqing Wang, Jinai Xue, Xiaoyun Jia and Runzhi Li

    Citation: Biotechnology for Biofuels 2021 14:76

    Content type: Research

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    The Correction to this article has been published in Biotechnology for Biofuels 2021 14:139

  28. Suberin is a hydrophobic biopolymer of significance in the production of biomass-derived materials and in biogeochemical cycling in terrestrial ecosystems. Here, we describe suberin structure and biosynthesis,...

    Authors: Anne E. Harman-Ware, Samuel Sparks, Bennett Addison and Udaya C. Kalluri

    Citation: Biotechnology for Biofuels 2021 14:75

    Content type: Review

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  29. The transition to a biobased economy involving the depolymerization and fermentation of renewable agro-industrial sources is a challenge that can only be met by achieving the efficient hydrolysis of biomass to...

    Authors: Sophie C. Brandt, Hévila Brognaro, Arslan Ali, Bernhard Ellinger, Katharina Maibach, Martin Rühl, Carsten Wrenger, Hartmut Schlüter, Wilhelm Schäfer, Christian Betzel, Stefan Janssen and Martin Gand

    Citation: Biotechnology for Biofuels 2021 14:74

    Content type: Research

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  30. Rhodotorula toruloides is a robust producer of triacylglycerol owing to its fast growth rate and strong metabolic flux under conditions of high cell density fermentation. However, the molecular basis of fatty aci...

    Authors: Yanbin Liu, Chong Mei John Koh, Sihui Amy Yap, Lin Cai and Lianghui Ji

    Citation: Biotechnology for Biofuels 2021 14:73

    Content type: Research

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  31. In view of the natural resistance of hemicelluloses in lignocellulosic biomass on bioconversion of cellulose into fermentable sugars, alkali extraction is considered as an effective method for gradually fracti...

    Authors: Shao-Fei Sun, Jing Yang, Da-Wei Wang, Hai-Yan Yang, Shao-Ni Sun and Zheng-Jun Shi

    Citation: Biotechnology for Biofuels 2021 14:72

    Content type: Research

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  32. An amendment to this paper has been published and can be accessed via the original article.

    Authors: Xiao Han, Yi An, Yangyan Zhou, Chao Liu, Weilun Yin and Xinli Xia

    Citation: Biotechnology for Biofuels 2021 14:71

    Content type: Correction

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    The original article was published in Biotechnology for Biofuels 2020 13:139

  33. Oxidation and peroxidation of lipids in microorganisms result in increased levels of intracellular reactive oxygen species (ROS) and reactive aldehydes, and consequent reduction of cell growth and lipid accumu...

    Authors: Xiao Han, Zhaohui Li, Ying Wen and Zhi Chen

    Citation: Biotechnology for Biofuels 2021 14:70

    Content type: Research

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  34. Methods to produce XOS have been intensively investigated, including enzymatic hydrolysis, steam explosion, and acid hydrolysis. Acid hydrolysis is currently the most widely used method to produce XOS due to i...

    Authors: Rou Cao, Xinlu Liu, Jianming Guo and Yong Xu

    Citation: Biotechnology for Biofuels 2021 14:69

    Content type: Research

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  35. A single-step ethanol production is the combination of raw cassava starch hydrolysis and fermentation. For the development of raw starch consolidated bioprocessing technologies, this research was to investigat...

    Authors: Morakot Krajang, Kwanruthai Malairuang, Jatuporn Sukna, Krongchan Rattanapradit and Saethawat Chamsart

    Citation: Biotechnology for Biofuels 2021 14:68

    Content type: Research

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  36. Cumulative reported evidence has indicated that renewable feedstocks are a promising alternative source to fossil platforms for the production of fuels and chemicals. In that regard, the development of new, hi...

    Authors: Gabriel Orlando Ferrero, Edgar Maximiliano Sánchez Faba and Griselda Alejandra Eimer

    Citation: Biotechnology for Biofuels 2021 14:67

    Content type: Research

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  37. Epidermal cell walls have special structural and biological roles in the life of the plant. Typically they are multi-ply structures encrusted with waxes and cutin which protect the plant from dehydration and p...

    Authors: Liza A. Wilson, Fabien Deligey, Tuo Wang and Daniel J. Cosgrove

    Citation: Biotechnology for Biofuels 2021 14:66

    Content type: Methodology

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  38. Crude glycerol (CG) and hemicellulose hydrolysate (HH) are low—value side-products of biodiesel transesterification and pulp—and paper industry or lignocellulosic ethanol production, respectively, which can be...

    Authors: Mikolaj Chmielarz, Johanna Blomqvist, Sabine Sampels, Mats Sandgren and Volkmar Passoth

    Citation: Biotechnology for Biofuels 2021 14:65

    Content type: Research

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  39. The production of microalgal biofuels, despite their sustainable and renowned potential, is not yet cost-effective compared to current conventional fuel technologies. However, the biorefinery concept increases...

    Authors: Gonzalo M. Figueroa-Torres, Jon K. Pittman and Constantinos Theodoropoulos

    Citation: Biotechnology for Biofuels 2021 14:64

    Content type: Research

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  40. Conventional aqueous dilute sulfuric acid (DSA) pretreatment of lignocellulosic biomass facilitates hemicellulose solubilization and can improve subsequent enzymatic digestibility of cellulose to fermentable g...

    Authors: Abhishek S. Patri, Ramya Mohan, Yunqiao Pu, Chang G. Yoo, Arthur J. Ragauskas, Rajeev Kumar, David Kisailus, Charles M. Cai and Charles E. Wyman

    Citation: Biotechnology for Biofuels 2021 14:63

    Content type: Research

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  41. The filamentous fungus Trichoderma reesei is one of the best producers of cellulase and has been widely studied for the production of cellulosic ethanol and bio-based products. We previously reported that Mn2+ an...

    Authors: Yumeng Chen, Xingjia Fan, Xinqing Zhao, Yaling Shen, Xiangyang Xu, Liujing Wei, Wei Wang and Dongzhi Wei

    Citation: Biotechnology for Biofuels 2021 14:62

    Content type: Research

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  42. Textile industry represents one prevalent activity worldwide, generating large amounts of highly contaminated and rich in azo dyes wastewater, with severe effects on natural ecosystems and public health. Howev...

    Authors: Sameh Samir Ali, Rania Al-Tohamy, Eleni Koutra, Michael Kornaros, Maha Khalil, Tamer Elsamahy, Mohamed El-Shetehy and Jianzhong Sun

    Citation: Biotechnology for Biofuels 2021 14:61

    Content type: Research

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  43. Miscanthus is a commercial lignocellulosic biomass crop owing to its high biomass productivity, resilience and photosynthetic capacity at low temperature. These qualities make Miscanthus a particularly good candi...

    Authors: Jose J. De Vega, Abel Teshome, Manfred Klaas, Jim Grant, John Finnan and Susanne Barth

    Citation: Biotechnology for Biofuels 2021 14:60

    Content type: Research

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  44. Pyrolysis-molecular beam mass spectrometry (py-MBMS) analysis of a pedigree of Populus trichocarpa was performed to study the phenotypic plasticity and heritability of lignin content and lignin monomer compositio...

    Authors: Anne E. Harman-Ware, David Macaya-Sanz, Chanaka Roshan Abeyratne, Crissa Doeppke, Kathleen Haiby, Gerald A. Tuskan, Brian Stanton, Stephen P. DiFazio and Mark F. Davis

    Citation: Biotechnology for Biofuels 2021 14:59

    Content type: Research

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  45. Multiple analytical methods have been developed to determine the ratios of aromatic lignin units, particularly the syringyl/guaiacyl (S/G) ratio, of lignin biopolymers in plant cell walls. Chemical degradation...

    Authors: Renee M. Happs, Bennett Addison, Crissa Doeppke, Bryon S. Donohoe, Mark F. Davis and Anne E. Harman-Ware

    Citation: Biotechnology for Biofuels 2021 14:58

    Content type: Research

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  46. Heterotrophic single-cell oils (SCOs) are one potential replacement to lipid-derived biofuels sourced from first-generation crops such as palm oil. However, despite a large experimental research effort in this...

    Authors: Eleni E. Karamerou, Sophie Parsons, Marcelle C. McManus and Christopher J. Chuck

    Citation: Biotechnology for Biofuels 2021 14:57

    Content type: Research

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  47. Slow degradation kinetics of long-chain fatty acids (LCFA) and their accumulation in anaerobic digesters disrupt methanogenic activity and biogas production at high loads of waste lipids. In this study, we eva...

    Authors: Sepehr Shakeri Yekta, Tong Liu, Thuane Mendes Anacleto, Mette Axelsson Bjerg, Luka Šafarič, Xavier Goux, Anna Karlsson, Annika Björn and Anna Schnürer

    Citation: Biotechnology for Biofuels 2021 14:56

    Content type: Research

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  48. Pretreatments are commonly used to facilitate the deconstruction of lignocellulosic biomass to its component sugars and aromatics. Previously, we showed that iron ions can be used as co-catalysts to reduce the...

    Authors: Chien-Yuan Lin, Bryon S. Donohoe, Yannick J. Bomble, Haibing Yang, Manal Yunes, Nicholas S. Sarai, Todd Shollenberger, Stephen R. Decker, Xiaowen Chen, Maureen C. McCann, Melvin P. Tucker, Hui Wei and Michael E. Himmel

    Citation: Biotechnology for Biofuels 2021 14:55

    Content type: Research

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