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  1. Consolidated bioprocessing, which combines saccharolytic and fermentative abilities in a single microorganism, is receiving increased attention to decrease environmental and economic costs in lignocellulosic b...

    Authors: Joana T. Cunha, Aloia Romaní, Kentaro Inokuma, Björn Johansson, Tomohisa Hasunuma, Akihiko Kondo and Lucília Domingues

    Citation: Biotechnology for Biofuels 2020 13:138

    Content type: Research

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  2. Trichoderma reesei is one of the best-known cellulolytic organisms, producing large quantities of a complete set of extracellular cellulases and hemicellulases for the degradation of lignocellulosic substances. H...

    Authors: Yun Luo, Mari Valkonen, Raymond E. Jackson, Jonathan M. Palmer, Aditya Bhalla, Igor Nikolaev, Markku Saloheimo and Michael Ward

    Citation: Biotechnology for Biofuels 2020 13:137

    Content type: Research

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  3. Cellobiohydrolase from glycoside hydrolase family 7 is a major component of commercial enzymatic mixtures for lignocellulosic biomass degradation. For many years, Trichoderma reesei Cel7A (TrCel7A) has served as ...

    Authors: Bartłomiej M. Kołaczkowski, Kay S. Schaller, Trine Holst Sørensen, Günther H. J. Peters, Kenneth Jensen, Kristian B. R. M. Krogh and Peter Westh

    Citation: Biotechnology for Biofuels 2020 13:136

    Content type: Research

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  4. Lytic polysaccharide monooxygenases (LPMOs) are often studied in simple models involving activity measurements of a single LPMO or a blend thereof with hydrolytic enzymes towards an insoluble substrate. Howeve...

    Authors: Majid Haddad Momeni, Maria Louise Leth, Claus Sternberg, Erwin Schoof, Maike Wennekers Nielsen, Jesper Holck, Christopher T. Workman, Jakob Blæsbjerg Hoof and Maher Abou Hachem

    Citation: Biotechnology for Biofuels 2020 13:135

    Content type: Research

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  5. Brassica napus is one of the most important oilseed crops, and also an important biofuel plant due to its low air pollution and renewability. Growth period are important traits that affect yield and are crucial f...

    Authors: Tengyue Wang, Lijuan Wei, Jia Wang, Ling Xie, Yang Yang Li, Shuyao Ran, Lanyang Ren, Kun Lu, Jiana Li, Michael P. Timko and Liezhao Liu

    Citation: Biotechnology for Biofuels 2020 13:134

    Content type: Research

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  6. Lupeol exhibits novel physiological and pharmacological activities, such as anticancer and immunity-enhancing activities. However, cytotoxicity remains a challenge for triterpenoid overproduction in microbial ...

    Authors: Jin-Lai Zhang, Qiu-Yan Bai, Yang-Zi Peng, Jie Fan, Cong-Cong Jin, Ying-Xiu Cao and Ying-Jin Yuan

    Citation: Biotechnology for Biofuels 2020 13:133

    Content type: Research

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  7. Fumarate is a multifunctional dicarboxylic acid in the tricarboxylic acid cycle, but microbial engineering for fumarate production is limited by the transmission efficiency of its biosynthetic pathway.

    Authors: Xiulai Chen, Danlei Ma, Jia Liu, Qiuling Luo and Liming Liu

    Citation: Biotechnology for Biofuels 2020 13:132

    Content type: Research

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  8. Docosahexaenoic acid (DHA) is an important omega-3 long-chain polyunsaturated fatty acid that has a variety of physiological functions for infant development and human health. Although metabolic engineering wa...

    Authors: Xiao Han, Zhunan Zhao, Ying Wen and Zhi Chen

    Citation: Biotechnology for Biofuels 2020 13:131

    Content type: Research

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  9. Nitrogen (N) starvation in algae induces a variety of structural and metabolic changes including accumulation of triacylglycerol (TAG). Given the promising prospect of using algae as feedstock for biofuel prod...

    Authors: Feifei Xu and Junmin Pan

    Citation: Biotechnology for Biofuels 2020 13:129

    Content type: Research

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  10. Chromochloris zofingiensis, an oleaginous microalga, is a promising feedstock for the co-production of triacylglycerol (TAG)-based biodiesel and the high-value product astaxanthin. To reveal the molecular mechani...

    Authors: Xuemei Mao, Yongmin Lao, Han Sun, Xiaojie Li, Jianfeng Yu and Feng Chen

    Citation: Biotechnology for Biofuels 2020 13:128

    Content type: Research

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  11. Heterotrophic microbial oils are potentially a more sustainable alternative to vegetable or fossil oils for food and fuel applications. However, as almost all work in the area is conducted on the laboratory sc...

    Authors: Felix Abeln, Robert H. Hicks, Hadiza Auta, Mauro Moreno-Beltrán, Luca Longanesi, Daniel A. Henk and Christopher J. Chuck

    Citation: Biotechnology for Biofuels 2020 13:127

    Content type: Research

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  12. High acetic acid tolerance is of major importance in industrial yeast strains used for second-generation bioethanol production, because of the high acetic acid content of lignocellulose hydrolysates. It is als...

    Authors: Marija Stojiljkovic, María R. Foulquié-Moreno and Johan M. Thevelein

    Citation: Biotechnology for Biofuels 2020 13:126

    Content type: Research

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  13. Microbial biorefinery approaches are beginning to define renewable and sustainable routes to clean-burning and non-fossil fuel-derived gaseous alkanes (known as ‘bio-LPG’). The most promising strategies have u...

    Authors: Mohamed Amer, Robin Hoeven, Paul Kelly, Matthew Faulkner, Michael H. Smith, Helen S. Toogood and Nigel S. Scrutton

    Citation: Biotechnology for Biofuels 2020 13:125

    Content type: Research

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  14. The current production of bioethanol based on lignocellulosic biomass (LCB) highly depends on thermostable enzymes and extremophiles owing to less risk of contamination. Thermophilic bacterial cellulases are p...

    Authors: Meena Ganesan, Remitha Mathivani Vinayakamoorthy, Sugitha Thankappan, Iniyakumar Muniraj and Sivakumar Uthandi

    Citation: Biotechnology for Biofuels 2020 13:124

    Content type: Research

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  15. Microbial cell factories are usually engineered and employed for cultivations that combine product synthesis with growth. Such a strategy inevitably invests part of the substrate pool towards the generation of...

    Authors: Enrico Orsi, Ioannis Mougiakos, Wilbert Post, Jules Beekwilder, Marco Dompè, Gerrit Eggink, John van der Oost, Servé W. M. Kengen and Ruud A. Weusthuis

    Citation: Biotechnology for Biofuels 2020 13:123

    Content type: Research

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  16. Soil-derived exogenous ash (EA) poses a challenge toward lignocellulosic autohydrolysis due to its buffering capacity. Previous works focusing on this phenomenon have failed to also investigate the role that s...

    Authors: Xinxing Wu, Wei Tang, Chen Huang, Caoxing Huang, Chenhuan Lai and Qiang Yong

    Citation: Biotechnology for Biofuels 2020 13:122

    Content type: Research

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  17. Fungal beta-glucosidases (BGs) from glucoside hydrolase family 3 (GH3) are industrially important enzymes, which convert cellooligosaccharides into glucose; the end product of the cellulolytic process. They ar...

    Authors: Malene B. Keller, Trine H. Sørensen, Kristian B. R. M. Krogh, Mark Wogulis, Kim Borch and Peter Westh

    Citation: Biotechnology for Biofuels 2020 13:121

    Content type: Research

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  18. The development of microalgal strains for enhanced biomass and biofuel production has received increased attention. Moreover, strain development via metabolic engineering for commercial production is being con...

    Authors: Seungbeom Seo, Joon Kim, Jun-Woo Lee, Onyou Nam, Kwang Suk Chang and EonSeon Jin

    Citation: Biotechnology for Biofuels 2020 13:120

    Content type: Research

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  19. The use of cyanobacteria and microalgae as cell factories to produce biofuels and added-value bioproducts has received great attention during the last two decades. Important investments have been made by publi...

    Authors: Sandra Isabel González-Morales, Navid Berenice Pacheco-Gutiérrez, Carlos A. Ramírez-Rodríguez, Alethia A. Brito-Bello, Priscila Estrella-Hernández, Luis Herrera-Estrella and Damar L. López-Arredondo

    Citation: Biotechnology for Biofuels 2020 13:119

    Content type: Research

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  20. Lignocellulose is the most abundant biomass on earth with an annual production of about 2 × 1011 tons. It is an inedible renewable carbonaceous resource that is very rich in pentose and hexose sugars. The ability...

    Authors: Jean Marie Francois, Ceren Alkim and Nicolas Morin

    Citation: Biotechnology for Biofuels 2020 13:118

    Content type: Review

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  21. Mannitol is a C(6) polyol that is used in the food and medical sector as a sweetener and antioxidant, respectively. The sustainable production of mannitol, especially via the direct conversion of CO2 by photosynt...

    Authors: Wenyang Wu, Wei Du, Ruth Perez Gallego, Klaas J. Hellingwerf, Aniek D. van der Woude and Filipe Branco dos Santos

    Citation: Biotechnology for Biofuels 2020 13:117

    Content type: Research

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  22. Global resource reallocation is an established critical strategy through which organisms deal with environmental stress. The regulation of intracellular lipid storage or utilization is one of the most importan...

    Authors: Hengqian Lu, Haiqin Chen, Xin Tang, Qin Yang, Hao Zhang, Yong Q. Chen and Wei Chen

    Citation: Biotechnology for Biofuels 2020 13:116

    Content type: Research

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  23. N-Acetyl glucosamine (GlcNAc) and N-Acetyl chitooligosaccharides (N-Acetyl COSs) exhibit many biological activities, and have been widely used in the pharmaceutical, agriculture, food, and chemical industries. Pa...

    Authors: Alei Zhang, Xiaofang Mo, Ning Zhou, Yingying Wang, Guoguang Wei, Jie Chen, Kequan Chen and Pingkai Ouyang

    Citation: Biotechnology for Biofuels 2020 13:115

    Content type: Research

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  24. Aldehyde decarbonylases (ADs), which convert acyl aldehydes into alkanes, supply promising solution for producing alkanes from renewable feedstock. However the instability of ADs impedes their further applicat...

    Authors: Yilan Liu, Jinjin Chen, Anna N. Khusnutdinova, Kevin Correia, Patrick Diep, Khorcheska A. Batyrova, Kayla Nemr, Robert Flick, Peter Stogios, Alexander F. Yakunin and Radhakrishnan Mahadevan

    Citation: Biotechnology for Biofuels 2020 13:114

    Content type: Research

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  25. Xylose is the most prevalent sugar available in hemicellulose fraction of lignocellulosic biomass (LCB) and of great interest for the green economy. Unfortunately, most of the cell factories cannot inherently ...

    Authors: Ashish A. Prabhu, Rodrigo Ledesma-Amaro, Carol Sze Ki Lin, Frederic Coulon, Vijay Kumar Thakur and Vinod Kumar

    Citation: Biotechnology for Biofuels 2020 13:113

    Content type: Research

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  26. The simultaneous and effective conversion of both pentose and hexose in fermentation is a critical and challenging task toward the lignocellulosic economy. This study aims to investigate the feasibility of an ...

    Authors: Ying Wang, Ka-Lai Chan, Mohamed Ali Abdel-Rahman, Kenji Sonomoto and Shao-Yuan Leu

    Citation: Biotechnology for Biofuels 2020 13:112

    Content type: Research

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  27. The industrial vinegar residue (VR) from solid-state fermentation, mainly cereals and their bran, will be a potential feedstock for future biofuels because of their low cost and easy availability. However, uti...

    Authors: Menglei Xia, Mingmeng Peng, Danni Xue, Yang Cheng, Caixia Li, Di Wang, Kai Lu, Yu Zheng, Ting Xia, Jia song and Min Wang

    Citation: Biotechnology for Biofuels 2020 13:111

    Content type: Research

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  28. The exposure of microalgae and plants to low UV-C radiation dosages can improve their biomass composition and stress tolerance. Despite UV-C sharing these effects with UV-A/B but at much lower dosages, UV-C se...

    Authors: Francisco Colina, María Carbó, Mónica Meijón, María Jesús Cañal and Luis Valledor

    Citation: Biotechnology for Biofuels 2020 13:110

    Content type: Research

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  29. Under nitrogen deficiency situation, Nannochloropsis spp. accumulate large amounts of lipids in the form of triacylglycerides (TAG). Mechanisms of this process from the perspective of transcriptome and metabolome...

    Authors: Wuxin You, Li Wei, Yanhai Gong, Mohamed El Hajjami, Jian Xu and Ansgar Poetsch

    Citation: Biotechnology for Biofuels 2020 13:109

    Content type: Research

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  30. Engineering yeast as a consolidated bioprocessing (CBP) microorganism by surface assembly of cellulosomes has been aggressively utilized for cellulosic ethanol production. However, most of the previous studies...

    Authors: Ce Dong, Jie Qiao, Xinping Wang, Wenli Sun, Lixia Chen, Shuntang Li, Ke Wu, Lixin Ma and Yi Liu

    Citation: Biotechnology for Biofuels 2020 13:108

    Content type: Research

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  31. Esterases and lipases hydrolyze short-chain esters and long-chain triglycerides, respectively, and therefore play essential roles in the synthesis and decomposition of ester bonds in the pharmaceutical and foo...

    Authors: Zhengyang Li, Long Li, Yingyi Huo, Zijun Chen, Yu Zhao, Jing Huang, Shuling Jian, Zhen Rong, Di Wu, Jianhua Gan, Xiaojian Hu, Jixi Li and Xue-Wei Xu

    Citation: Biotechnology for Biofuels 2020 13:107

    Content type: Research

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  32. The sorghum stem can be divided into the pith and rind parts with obvious differences in cell type and chemical composition, thus arising the different recalcitrance to enzyme hydrolysis and demand for differe...

    Authors: Dandan Li, Liangkun Long and Shaojun Ding

    Citation: Biotechnology for Biofuels 2020 13:106

    Content type: Research

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  33. Phototrophic purple non-sulfur bacteria (PNSB) have gained attention for their ability to produce a valuable clean energy source in the form biohydrogen via photofermentation of a wide variety of organic waste...

    Authors: Jan-Pierre du Toit and Robert W. M. Pott

    Citation: Biotechnology for Biofuels 2020 13:105

    Content type: Research

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  34. Industrial biotechnology will play an increasing role in creating a more sustainable global economy. For conventional aerobic bioprocesses supplying O2 can account for 15% of total production costs. Microbubbles ...

    Authors: Vijayendran Raghavendran, Joseph P. Webb, Michaël L. Cartron, Vicki Springthorpe, Tony R. Larson, Michael Hines, Hamza Mohammed, William B. Zimmerman, Robert K. Poole and Jeffrey Green

    Citation: Biotechnology for Biofuels 2020 13:104

    Content type: Research

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  35. A precise map of the metabolic fluxome, the closest surrogate to the physiological phenotype, is becoming progressively more important in the metabolic engineering of photosynthetic organisms for biofuel and b...

    Authors: Zhengdong Zhang, Zhentao Liu, Yafei Meng, Zhen Chen, Jiayu Han, Yimin Wei, Tie Shen, Yin Yi and Xiaoyao Xie

    Citation: Biotechnology for Biofuels 2020 13:103

    Content type: Methodology

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  36. Butyric acid is a platform chemical material, the production of which has been greatly stimulated by the diverse range of downstream applications in many industries. In particular, higher quality butyric acid ...

    Authors: Xia Hua, GenLai Du, Xin Zhou, Ali Nawaz, Ikram ul Haq and Yong Xu

    Citation: Biotechnology for Biofuels 2020 13:102

    Content type: Research

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  37. Ethanol-type fermentation, one of the fermentation types in mixed cultures of acidogenesis with obvious advantages such as low pH tolerance and high efficiency of H2 production, has attracted widespread attention...

    Authors: Zhen Li, Yu Lou, Jie Ding, Bing-Feng Liu, Guo-Jun Xie, Nan-Qi Ren and Defeng Xing

    Citation: Biotechnology for Biofuels 2020 13:101

    Content type: Research

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  38. Platform chemicals are essential to industrial processes. Used as starting materials for the manufacture of diverse products, their cheap availability and efficient sourcing are an industrial requirement. Incr...

    Authors: Pinar Karagoz, Ravneet Mandair, Jinesh Cherukkattu Manayil, Jai Lad, Katie Chong, Georgios Kyriakou, Adam F. Lee, Karen Wilson and Roslyn M. Bill

    Citation: Biotechnology for Biofuels 2020 13:100

    Content type: Research

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  39. As the most abundant polysaccharide in brown algae, alginate has become a promising economical material for bioethanol production. Recently, exo-type alginate lyases have received extensive attention because t...

    Authors: Luyao Tang, Ying Wang, Shan Gao, Hao Wu, Danni Wang, Wengong Yu and Feng Han

    Citation: Biotechnology for Biofuels 2020 13:99

    Content type: Research

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  40. Microalgae are widely be used in carbon sequestration, food supplements, natural pigments, polyunsaturated fatty acids, biofuel applications, and wastewater treatment. However, the difficulties incurred in alg...

    Authors: Mingcan Wu, Jing Li, Huan Qin, Anping Lei, Hui Zhu, Zhangli Hu and Jiangxin Wang

    Citation: Biotechnology for Biofuels 2020 13:98

    Content type: Research

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  41. During the chemical and biochemical decomposition of lignocellulosic biomasses, lignin is highly recalcitrant. Genetic transformation of plants to qualitatively and/or quantitatively modify lignin may reduce t...

    Authors: Shingo Sakamoto, Naofumi Kamimura, Yosuke Tokue, Miyuki T. Nakata, Masanobu Yamamoto, Shi Hu, Eiji Masai, Nobutaka Mitsuda and Shinya Kajita

    Citation: Biotechnology for Biofuels 2020 13:97

    Content type: Research

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  42. Succinic acid (SA), a valuable chemical compound with a broad range of industrial uses, has become a subject of global interest in recent years. The bio-based production of SA by highly efficient microbial pro...

    Authors: Hubert Szczerba, Elwira Komoń-Janczara, Karolina Dudziak, Adam Waśko and Zdzisław Targoński

    Citation: Biotechnology for Biofuels 2020 13:96

    Content type: Research

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  43. Lytic polysaccharide monooxygenases (LPMOs) are powerful enzymes that oxidatively cleave plant cell wall polysaccharides. LPMOs classified as fungal Auxiliary Activities family 9 (AA9) have been mainly studied...

    Authors: Peicheng Sun, Christophe V. F. P. Laurent, Stefan Scheiblbrandner, Matthias Frommhagen, Dimitrios Kouzounis, Mark G. Sanders, Willem J. H. van Berkel, Roland Ludwig and Mirjam A. Kabel

    Citation: Biotechnology for Biofuels 2020 13:95

    Content type: Research

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  44. Biohydrogen production from lignocellulose has become an important hydrogen production method due to its diversity, renewability, and cheapness. Overexpression of the formate hydrogen lyase activator (fhlA) gene ...

    Authors: Qin Zhang, Shaolin You, Yanbin Li, Xiaowei Qu and Hui Jiang

    Citation: Biotechnology for Biofuels 2020 13:94

    Content type: Research

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  45. The path for the development of hypersecreting strains of Trichoderma reesei capable of producing industrially relevant enzyme titers remains elusive despite over 70 years of research and industrial utilization. ...

    Authors: Lucas Miranda Fonseca, Lucas Salera Parreiras and Mario Tyago Murakami

    Citation: Biotechnology for Biofuels 2020 13:93

    Content type: Research

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  46. The development of alternative pathways for sustainable fuel production is a crucial task for politics, industry and research, since the current use of fossil fuels contributes to resource depletion and climat...

    Authors: Jessica Varela Villarreal, Cecilia Burgués and Christine Rösch

    Citation: Biotechnology for Biofuels 2020 13:92

    Content type: Research

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  47. Although bioethanol production has been gaining worldwide attention as an alternative to fossil fuel, ethanol productivities and yields are still limited due to the susceptibility of fermentation microorganism...

    Authors: Ifeanyi A. Ndubuisi, Qijian Qin, Guiyan Liao, Bin Wang, Anene N. Moneke, James C. Ogbonna, Cheng Jin and Wenxia Fang

    Citation: Biotechnology for Biofuels 2020 13:91

    Content type: Research

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  48. Brassica rapa is an important oilseed and vegetable crop species and is the A subgenome donor of two important oilseed Brassica crops, Brassica napus and Brassica juncea. Although seed size (SZ), seed color (SC),...

    Authors: Yue Niu, Limin Wu, Yanhua Li, Hualei Huang, Mingchao Qian, Wei Sun, Hong Zhu, Yuanfang Xu, Yonghai Fan, Umer Mahmood, Benbo Xu, Kai Zhang, Cunmin Qu, Jiana Li and Kun Lu

    Citation: Biotechnology for Biofuels 2020 13:90

    Content type: Research

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  49. Cyanobacteria, a group of photosynthetic prokaryotes, are being increasingly explored for direct conversion of carbon dioxide to useful chemicals. However, efforts to engineer these photoautotrophs have result...

    Authors: Shinjinee Sengupta, Damini Jaiswal, Annesha Sengupta, Shikha Shah, Shruti Gadagkar and Pramod P. Wangikar

    Citation: Biotechnology for Biofuels 2020 13:89

    Content type: Research

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