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  1. Woody biomass has been considered as a promising feedstock for biofuel production via thermochemical conversion technologies such as fast pyrolysis. Extensive Life Cycle Assessment studies have been completed ...

    Authors: Kai Lan, Longwen Ou, Sunkyu Park, Stephen S. Kelley, Prakash Nepal, Hoyoung Kwon, Hao Cai and Yuan Yao
    Citation: Biotechnology for Biofuels 2021 14:191
  2. In the oilseed crop Brassica napus (rapeseed), various metabolic processes influence seed oil content, oil quality, and biological yield. However, the role of plastid membrane proteins in these traits has not bee...

    Authors: Zhongchun Xiao, Fang Tang, Liyuan Zhang, Shengting Li, Shufeng Wang, Qiang Huo, Bo Yang, Chao Zhang, Daojie Wang, Qing Li, Lijuan Wei, Tao Guo, Cunmin Qu, Kun Lu, Yanfeng Zhang, Liang Guo…
    Citation: Biotechnology for Biofuels 2021 14:190
  3. Lignin is a complex aromatic heteropolymer comprising 15–30% dry weight of the lignocellulose. The complex structural characteristic of lignin renders it difficult for value-added utilization. Exploring effici...

    Authors: Jiangshan Ma, Qiang Li, Yujie Wu, Huimin Yue, Yanghong Zhang, Jiashun Zhang, Muling Shi, Sixian Wang and Gao-Qiang Liu
    Citation: Biotechnology for Biofuels 2021 14:189
  4. Sugar alcohols are widely used as low-calorie sweeteners in the food and pharmaceutical industries. They can also be transformed into platform chemicals. Yarrowia lipolytica, an oleaginous yeast, is a promising h...

    Authors: Sujit Sadashiv Jagtap, Ashwini Ashok Bedekar, Vijay Singh, Yong-Su Jin and Christopher V. Rao
    Citation: Biotechnology for Biofuels 2021 14:188
  5. The industrial production of various alcohols from organic carbon compounds may be performed at high rates and with a low risk of contamination using thermophilic microorganisms as whole-cell catalysts. Thermoana...

    Authors: Lisa Hitschler, Laura Sofie Nissen, Michelle Kuntz and Mirko Basen
    Citation: Biotechnology for Biofuels 2021 14:187
  6. Consolidated bioprocessing (CBP) technique is a promising strategy for biorefinery construction, producing bulk chemicals directly from plant biomass without extra hydrolysis steps. Fixing and channeling CO2 into...

    Authors: Jingen Li, Bingchen Chen, Shuying Gu, Zhen Zhao, Qian Liu, Tao Sun, Yongli Zhang, Taju Wu, Defei Liu, Wenliang Sun and Chaoguang Tian
    Citation: Biotechnology for Biofuels 2021 14:186
  7. Recent research articles indicate that direct interspecies electron transfer (DIET) is an alternative metabolic route for methanogenic archaea that improves microbial methane productivity. It has been shown th...

    Authors: Kerstin Heitkamp, Adriel Latorre-PĂ©rez, Sven Nefigmann, Helena Gimeno-Valero, Cristina Vilanova, Efri Jahmad and Christian Abendroth
    Citation: Biotechnology for Biofuels 2021 14:185
  8. The green microalga Chromochloris zofingiensis is capable of producing high levels of triacylglycerol rich in C18 unsaturated fatty acids (UFAs). FA desaturation degree is regulated by FA desaturases (FADs). Neve...

    Authors: Tao Wu, Lihua Yu, Yu Zhang and Jin Liu
    Citation: Biotechnology for Biofuels 2021 14:184
  9. Endo-β-1,4-galactanases are glycoside hydrolases (GH) from the GH53 family belonging to the largest clan of GHs, clan GH-A. GHs are ubiquitous and involved in a myriad of biological functions as well as being ...

    Authors: Sebastian J. Muderspach, Folmer Fredslund, Verena Volf, Jens-Christian Navarro Poulsen, Thomas H. Blicher, Mads Hartvig Clausen, Kim Krighaar Rasmussen, Kristian B. R. M. Krogh, Kenneth Jensen and Leila Lo Leggio
    Citation: Biotechnology for Biofuels 2021 14:183
  10. Bio-hydrogen production via dark fermentation of low-value waste is a potent and simple mean of recovering energy, maximising the harvesting of reducing equivalents to produce the cleanest fuel amongst renewab...

    Authors: M. Arizzi, S. Morra, G. Gilardi, M. Pugliese, M. L. Gullino and F. Valetti
    Citation: Biotechnology for Biofuels 2021 14:182
  11. Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production of microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates h...

    Authors: Zhi-Peng Wang, Xin-Yue Zhang, Yan Ma, Jing-Run Ye, Jing Jiang, Hai-Ying Wang and Wei Chen
    Citation: Biotechnology for Biofuels 2021 14:181
  12. Microalgae are coming to the spotlight due to their potential applications in a wide number of fields ranging from the biofuel to the pharmaceutical sector. However, several factors such as low productivity, e...

    Authors: M. Giovannoni, I. Larini, V. Scafati, A. Scortica, M. Compri, D. Pontiggia, G. Zapparoli, N. Vitulo, M. Benedetti and B. Mattei
    Citation: Biotechnology for Biofuels 2021 14:180
  13. Environmental factors, such as weather extremes, have the potential to cause adverse effects on plant biomass quality and quantity. Beyond adversely affecting feedstock yield and composition, which have been e...

    Authors: Meenaa Chandrasekar, Leela Joshi, Karleigh Krieg, Sarvada Chipkar, Emily Burke, Derek J. Debrauske, Kurt D. Thelen, Trey K. Sato and Rebecca G. Ong
    Citation: Biotechnology for Biofuels 2021 14:179
  14. In terms of global demand, rapeseed is the third-largest oilseed crop after soybeans and palm, which produces vegetable oil for human consumption and biofuel for industrial production. Roots are vital organs f...

    Authors: Keqi Li, Jie Wang, Lieqiong Kuang, Ze Tian, Xinfa Wang, Xiaoling Dun, Jinxing Tu and Hanzhong Wang
    Citation: Biotechnology for Biofuels 2021 14:178
  15. To create an ideotype woody bioenergy crop with desirable growth and biomass properties, we utilized the viral 2A-meidated bicistronic expression strategy to express both PtrMYB3 (MYB46 ortholog of Populus tricho...

    Authors: Jin-Seong Cho, Min-Ha Kim, Eun-Kyung Bae, Young-Im Choi, Hyung-Woo Jeon, Kyung-Hwan Han and Jae-Heung Ko
    Citation: Biotechnology for Biofuels 2021 14:177
  16. The efficiency of biological systems as an option for pretreating lignocellulosic biomass has to be improved to make the process practical. Fungal treatment with manganese (Mn) addition for improving lignocell...

    Authors: Xiao Fu, Jialong Zhang, Xiangyu Gu, Hongbo Yu and Shulin Chen
    Citation: Biotechnology for Biofuels 2021 14:176
  17. Lignification of secondary cell walls is a major factor conferring recalcitrance of lignocellulosic biomass to deconstruction for fuels and chemicals. Genetic modification can reduce lignin content and enhance...

    Authors: Hasi Yu, Chang Liu and Richard A. Dixon
    Citation: Biotechnology for Biofuels 2021 14:175
  18. Isobutanol is an attractive biofuel with many advantages. Third-generation biorefineries that convert CO2 into bio-based fuels have drawn considerable attention due to their lower feedstock cost and more ecofrien...

    Authors: Xiao-Xi Wu, Jian-Wei Li, Su-Fang Xing, Hui-Ting Chen, Chao Song, Shu-Guang Wang and Zhen Yan
    Citation: Biotechnology for Biofuels 2021 14:174
  19. The fermentation of lignocellulose hydrolysates to ethanol requires robust xylose-capable Saccharomyces cerevisiae strains able to operate in the presence of microbial inhibitory stresses. This study aimed at dev...

    Authors: Bianca A. Brandt, Maria D. P. García-Aparicio, Johann F. Görgens and Willem H. van Zyl
    Citation: Biotechnology for Biofuels 2021 14:173
  20. Over the last few years, valorization of lignocellulosic biomass has been expanded beyond the production of second-generation biofuels to the synthesis of numerous platform chemicals to be used instead of thei...

    Authors: Grigorios Dedes, Anthi Karnaouri, Asimina A. Marianou, Konstantinos G. Kalogiannis, Chrysoula M. Michailof, Angelos A. Lappas and Evangelos Topakas
    Citation: Biotechnology for Biofuels 2021 14:172
  21. Sugarcane bagasse (SCB) is an abundant feedstock for second-generation bioethanol production. This complex biomass requires an array of carbohydrate active enzymes (CAZymes), mostly from filamentous fungi, for...

    Authors: Olusola A. Ogunyewo, Pooja Upadhyay, Girish H. Rajacharya, Omoaruemike E. Okereke, Laura Faas, Leonardo D. GĂłmez, Simon J. McQueen-Mason and Syed Shams Yazdani
    Citation: Biotechnology for Biofuels 2021 14:171
  22. Biogas can be upgraded to methane biologically by adding H2 to biogas reactors. The process is called biological methanation (BM) and can be done in situ in a regular biogas reactor or the biogas can be transferr...

    Authors: Radziah Wahid and Svein Jarle Horn
    Citation: Biotechnology for Biofuels 2021 14:170
  23. Filamentous fungi are excellent lignocellulose degraders, which they achieve through producing carbohydrate active enzymes (CAZymes). CAZyme production is highly orchestrated and gene expression analysis has g...

    Authors: Raphael Gabriel, Rebecca Mueller, Lena Floerl, Cynthia Hopson, Simon Harth, Timo Schuerg, Andre Fleissner and Steven W. Singer
    Citation: Biotechnology for Biofuels 2021 14:169
  24. Rhodospirillum rubrum is a purple non-sulphur bacterium that produces H2 by photofermentation of several organic compounds or by water gas-shift reaction during CO fermentation. Successful strategies for both pro...

    Authors: Alberto Rodríguez, Natalia Hernández-Herreros, José L. García and M. Auxiliadora Prieto
    Citation: Biotechnology for Biofuels 2021 14:168
  25. Plants inherently display a rich diversity in cell wall chemistry, as they synthesize an array of polysaccharides along with lignin, a polyphenolic that can vary dramatically in subunit composition and interun...

    Authors: Lisanne de Vries, Sydne Guevara-Rozo, MiJung Cho, Li-Yang Liu, Scott Renneckar and Shawn D. Mansfield
    Citation: Biotechnology for Biofuels 2021 14:167
  26. As the fifth major cereal crop originated from Africa, sorghum (Sorghum bicolor) has become a key C4 model organism for energy plant research. With the development of high-throughput detection technologies for va...

    Authors: Yuanming Liu, Zhonghuang Wang, Xiaoyuan Wu, Junwei Zhu, Hong Luo, Dongmei Tian, Cuiping Li, Jingchu Luo, Wenming Zhao, Huaiqing Hao and Hai-Chun Jing
    Citation: Biotechnology for Biofuels 2021 14:165
  27. Biomass recalcitrance is governed by various molecular and structural factors but the interplay between these multiscale factors remains unclear. In this study, hot water pretreatment (HWP) was applied to maiz...

    Authors: Amandine Leroy, Xavier Falourd, Loïc Foucat, Valérie Méchin, Fabienne Guillon and Gabriel Paës
    Citation: Biotechnology for Biofuels 2021 14:164
  28. As a potential source of polyunsaturated fatty acids (PUFA), Schizochytrium sp. has been widely used in industry for PUFA production. Polyketide synthase (PKS) cluster is supposed to be the primary way of PUFA sy...

    Authors: Yanyan Shi, Zhen Chen, Yixin Li, Xingyu Cao, Lijie Yang, Yiyuan Xu, Zhipeng Li and Ning He
    Citation: Biotechnology for Biofuels 2021 14:163
  29. As one of the major components of lignocellulosic biomass, lignin has been considered as the most abundant renewable aromatic feedstock in the world. Comparing with thermal or catalytic strategies for lignin d...

    Authors: Tangwu Cui, Bo Yuan, Haiwei Guo, Hua Tian, Weimin Wang, Yingqun Ma, Changzhi Li and Qiang Fei
    Citation: Biotechnology for Biofuels 2021 14:162
  30. Fungal glucose dehydrogenases (GDHs) are FAD-dependent enzymes belonging to the glucose-methanol-choline oxidoreductase superfamily. These enzymes are classified in the “Auxiliary Activity” family 3 (AA3) of t...

    Authors: Gabriele Cerutti, Elena Gugole, Linda Celeste Montemiglio, Annick Turbé-Doan, Dehbia Chena, David Navarro, Anne Lomascolo, François Piumi, Cécile Exertier, Ida Freda, Beatrice Vallone, Eric Record, Carmelinda Savino and Giuliano Sciara
    Citation: Biotechnology for Biofuels 2021 14:161
  31. Population increase and industrialization has resulted in high energy demand and consumptions, and presently, fossil fuels are the major source of staple energy, supplying 80% of the entire consumption. This h...

    Authors: Kehinde Oladoke Olatunji, Noor A. Ahmed and Oyetola Ogunkunle
    Citation: Biotechnology for Biofuels 2021 14:159
  32. Future expansion of corn-derived ethanol raises concerns of sustainability and competition with the food industry. Therefore, cellulosic biofuels derived from agricultural waste and dedicated energy crops are ...

    Authors: Shannon M. Hoffman, Maria Alvarez, Gilad Alfassi, Dmitry M. Rein, Sergio Garcia-Echauri, Yachin Cohen and José L. Avalos
    Citation: Biotechnology for Biofuels 2021 14:157
  33. Production of biodiesel from renewable sources such as inedible vegetable oils by enzymatic catalysis has been a hotspot but remains a challenge on the efficient use of an enzyme. COFs (Covalent Organic Framew...

    Authors: Zi-Wen Zhou, Chun-Xian Cai, Xiu Xing, Jun Li, Zu-E. Hu, Zong-Bo Xie, Na Wang and Xiao-Qi Yu
    Citation: Biotechnology for Biofuels 2021 14:156
  34. Saccharomyces cerevisiae is widely used in traditional brewing and modern fermentation industries to produce biofuels, chemicals and other bioproducts, but challenged by various harsh industrial conditions, such ...

    Authors: Yanfang Liu, Yuping Lin, Yufeng Guo, Fengli Wu, Yuanyuan Zhang, Xianni Qi, Zhen Wang and Qinhong Wang
    Citation: Biotechnology for Biofuels 2021 14:155
  35. The recalcitrance of lignocellulosic biomass is a major constraint to its high-value use at industrial scale. In nature, microbes play a crucial role in biomass degradation, nutrient recycling and ecosystem fu...

    Authors: Sivasamy Sethupathy, Gabriel Murillo Morales, Yixuan Li, Yongli Wang, Jianxiong Jiang, Jianzhong Sun and Daochen Zhu
    Citation: Biotechnology for Biofuels 2021 14:154
  36. Sugarcane processing roughly generates 54 million tonnes sugarcane bagasse (SCB)/year, making SCB an important material for upgrading to value-added molecules. In this study, an integrated scheme was developed...

    Authors: Leila Khaleghipour, Javier A. Linares-Pastén, Hamid Rashedi, Seyed Omid Ranaei Siadat, Andrius Jasilionis, Said Al-Hamimi, Roya R. R. Sardari and Eva Nordberg Karlsson
    Citation: Biotechnology for Biofuels 2021 14:153
  37. Sugarcane is an essential crop for sugar and ethanol production. Immediate processing of sugarcane is necessary after harvested because of rapid sucrose losses and deterioration of stalks. This study was condu...

    Authors: Na Peng, Ziting Yao, Ziting Wang, Jiangfeng Huang, Muhammad Tahir Khan, Baoshan Chen and Muqing Zhang
    Citation: Biotechnology for Biofuels 2021 14:152
  38. Hydrogen peroxide–acetic acid (HPAA) is widely used in pretreatment of lignocellulose because it has a good capability in selective delignification. However, high concentration (more than 60%) of HPAA increase...

    Authors: Peiyao Wen, Ying Zhang, Junjun Zhu, Yong Xu and Junhua Zhang
    Citation: Biotechnology for Biofuels 2021 14:151
  39. Ascomycetous yeasts from the kingdom fungi inhabit every biome in nature. While filamentous fungi have been studied extensively regarding their enzymatic degradation of the complex polymers comprising lignocel...

    Authors: Jonas L. Ravn, Martin K. M. Engqvist, Johan Larsbrink and Cecilia Geijer
    Citation: Biotechnology for Biofuels 2021 14:150
  40. In biological cells, promoters drive gene expression by specific binding of RNA polymerase. They determine the starting position, timing and level of gene expression. Therefore, rational fine-tuning of promote...

    Authors: Yu Zhao, Shiqi Liu, Zhihui Lu, Baixiang Zhao, Shuhui Wang, Cuiying Zhang, Dongguang Xiao, Jee Loon Foo and Aiqun Yu
    Citation: Biotechnology for Biofuels 2021 14:149
  41. Lipomyces starkeyi has been widely regarded as a promising oleaginous yeast with broad industrial application prospects because of its wide substrate spectrum, good adaption to fermentation inhibitors, excellent ...

    Authors: Wei Zhou, Yanan Wang, Junlu Zhang, Man Zhao, Mou Tang, Wenting Zhou and Zhiwei Gong
    Citation: Biotechnology for Biofuels 2021 14:148
  42. Plant monoterpenoids with structural diversities have extensive applications in food, cosmetics, pharmaceuticals, and biofuels. Due to the strong dependence on the geographical locations and seasonal annual gr...

    Authors: Dengwei Lei, Zetian Qiu, Jianjun Qiao and Guang-Rong Zhao
    Citation: Biotechnology for Biofuels 2021 14:147
  43. The model ethanologenic bacterium Zymomonas mobilis has many advantages for diverse biochemical production. Although the impact of temperature especially high temperature on the growth and ethanol production of Z...

    Authors: Runxia Li, Wei Shen, Yongfu Yang, Jun Du, Mian Li and Shihui Yang
    Citation: Biotechnology for Biofuels 2021 14:146
  44. Succinic acid (SA) is a crucial metabolic intermediate and platform chemical. Development of biobased processes to achieve sustainable SA production has attracted more and more attention in biotechnology indus...

    Authors: Zhennan Jiang, Zhiyong Cui, Ziwei Zhu, Yinghang Liu, Ya-jie Tang, Jin Hou and Qingsheng Qi
    Citation: Biotechnology for Biofuels 2021 14:145
  45. Identifying lignocellulose recalcitrant factors and exploring their genetic properties are essential for enhanced biomass enzymatic saccharification in bioenergy crops. Despite genetic modification of major wa...

    Authors: Zhen Hu, Youmei Wang, Jingyuan Liu, Yuqi Li, Yanting Wang, Jiangfeng Huang, Yuanhang Ai, Peng Chen, Yuqing He, Muhammad Nauman Aftab, Lingqiang Wang and Liangcai Peng
    Citation: Biotechnology for Biofuels 2021 14:144
  46. During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. H...

    Authors: Wenqian Lin, Jinlai Yang, Yayue Zheng, Caoxing Huang and Qiang Yong
    Citation: Biotechnology for Biofuels 2021 14:143
  47. In plants, a large diversity of polysaccharides comprise the cell wall. Each major type of plant cell wall polysaccharide, including cellulose, hemicellulose, and pectin, has distinct structures and functions ...

    Authors: William J. Barnes, Sabina Koj, Ian M. Black, Stephanie A. Archer-Hartmann, Parastoo Azadi, Breeanna R. Urbanowicz, Maria J. Peña and Malcolm A. O’Neill
    Citation: Biotechnology for Biofuels 2021 14:142