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  1. Conventional aqueous dilute sulfuric acid (DSA) pretreatment of lignocellulosic biomass facilitates hemicellulose solubilization and can improve subsequent enzymatic digestibility of cellulose to fermentable g...

    Authors: Abhishek S. Patri, Ramya Mohan, Yunqiao Pu, Chang G. Yoo, Arthur J. Ragauskas, Rajeev Kumar, David Kisailus, Charles M. Cai and Charles E. Wyman

    Citation: Biotechnology for Biofuels 2021 14:63

    Content type: Research

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  2. The filamentous fungus Trichoderma reesei is one of the best producers of cellulase and has been widely studied for the production of cellulosic ethanol and bio-based products. We previously reported that Mn2+ an...

    Authors: Yumeng Chen, Xingjia Fan, Xinqing Zhao, Yaling Shen, Xiangyang Xu, Liujing Wei, Wei Wang and Dongzhi Wei

    Citation: Biotechnology for Biofuels 2021 14:62

    Content type: Research

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  3. Textile industry represents one prevalent activity worldwide, generating large amounts of highly contaminated and rich in azo dyes wastewater, with severe effects on natural ecosystems and public health. Howev...

    Authors: Sameh Samir Ali, Rania Al-Tohamy, Eleni Koutra, Michael Kornaros, Maha Khalil, Tamer Elsamahy, Mohamed El-Shetehy and Jianzhong Sun

    Citation: Biotechnology for Biofuels 2021 14:61

    Content type: Research

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  4. Miscanthus is a commercial lignocellulosic biomass crop owing to its high biomass productivity, resilience and photosynthetic capacity at low temperature. These qualities make Miscanthus a particularly good candi...

    Authors: Jose J. De Vega, Abel Teshome, Manfred Klaas, Jim Grant, John Finnan and Susanne Barth

    Citation: Biotechnology for Biofuels 2021 14:60

    Content type: Research

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  5. Pyrolysis-molecular beam mass spectrometry (py-MBMS) analysis of a pedigree of Populus trichocarpa was performed to study the phenotypic plasticity and heritability of lignin content and lignin monomer compositio...

    Authors: Anne E. Harman-Ware, David Macaya-Sanz, Chanaka Roshan Abeyratne, Crissa Doeppke, Kathleen Haiby, Gerald A. Tuskan, Brian Stanton, Stephen P. DiFazio and Mark F. Davis

    Citation: Biotechnology for Biofuels 2021 14:59

    Content type: Research

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  6. Multiple analytical methods have been developed to determine the ratios of aromatic lignin units, particularly the syringyl/guaiacyl (S/G) ratio, of lignin biopolymers in plant cell walls. Chemical degradation...

    Authors: Renee M. Happs, Bennett Addison, Crissa Doeppke, Bryon S. Donohoe, Mark F. Davis and Anne E. Harman-Ware

    Citation: Biotechnology for Biofuels 2021 14:58

    Content type: Research

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  7. Heterotrophic single-cell oils (SCOs) are one potential replacement to lipid-derived biofuels sourced from first-generation crops such as palm oil. However, despite a large experimental research effort in this...

    Authors: Eleni E. Karamerou, Sophie Parsons, Marcelle C. McManus and Christopher J. Chuck

    Citation: Biotechnology for Biofuels 2021 14:57

    Content type: Research

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  8. Slow degradation kinetics of long-chain fatty acids (LCFA) and their accumulation in anaerobic digesters disrupt methanogenic activity and biogas production at high loads of waste lipids. In this study, we eva...

    Authors: Sepehr Shakeri Yekta, Tong Liu, Thuane Mendes Anacleto, Mette Axelsson Bjerg, Luka Šafarič, Xavier Goux, Anna Karlsson, Annika Björn and Anna Schnürer

    Citation: Biotechnology for Biofuels 2021 14:56

    Content type: Research

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  9. Pretreatments are commonly used to facilitate the deconstruction of lignocellulosic biomass to its component sugars and aromatics. Previously, we showed that iron ions can be used as co-catalysts to reduce the...

    Authors: Chien-Yuan Lin, Bryon S. Donohoe, Yannick J. Bomble, Haibing Yang, Manal Yunes, Nicholas S. Sarai, Todd Shollenberger, Stephen R. Decker, Xiaowen Chen, Maureen C. McCann, Melvin P. Tucker, Hui Wei and Michael E. Himmel

    Citation: Biotechnology for Biofuels 2021 14:55

    Content type: Research

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  10. Yellow nutsedge is a unique plant species that can accumulate up to 35% oil of tuber dry weight, perhaps the highest level observed in the tuber tissues of plant kingdom. To gain insight into the molecular mec...

    Authors: Hongying Ji, Dantong Liu and Zhenle Yang

    Citation: Biotechnology for Biofuels 2021 14:54

    Content type: Research

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  11. Sugar content is critically important in determining sugar crop productivity. However, improvement in sugar content has been stagnant among sugar crops for decades. Sorghum, especially sweet sorghum with high ...

    Authors: Guoquan Liu, Yan Zhang, Hao Gong, Shan Li, Yunrong Pan, Christopher Davis, Hai-Chun Jing, Luguang Wu and Ian D. Godwin

    Citation: Biotechnology for Biofuels 2021 14:53

    Content type: Research

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  12. The amount of carbon dioxide in the atmosphere has been on the rise for more than a century. Bioenergy crops are seen by the Intergovernmental Panel on Climate Change as an essential part of the solution to ad...

    Authors: Obste Therasme, Timothy A. Volk, Mark H. Eisenbies, Thomas E. Amidon and Marie-Odile Fortier

    Citation: Biotechnology for Biofuels 2021 14:52

    Content type: Research

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  13. Lytic polysaccharide monooxygenases (LPMOs) are important industrial enzymes known for their catalytic degradation of recalcitrant polymers such as cellulose or chitin. Their activity can be measured by length...

    Authors: Søren Brander, Stine Lausten, Johan Ø. Ipsen, Kristoffer B. Falkenberg, Andreas B. Bertelsen, Morten H. H. Nørholm, Lars H. Østergaard and Katja S. Johansen

    Citation: Biotechnology for Biofuels 2021 14:51

    Content type: Methodology

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  14. Hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase (HCT) is a central enzyme of the so-called “esters” pathway to monolignols. As originally envisioned, HCT functions twice in this pathway, to form c...

    Authors: Juan Carlos Serrani-Yarce, Luis Escamilla-Trevino, Jaime Barros, Lina Gallego-Giraldo, Yunqiao Pu, Art Ragauskas and Richard A. Dixon

    Citation: Biotechnology for Biofuels 2021 14:50

    Content type: Research

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  15. Ionic liquids (ILs) are promising pretreatment solvents for lignocellulosic biomass, but are largely prepared from petroleum precursors. Benzaldehydes from depolymerized lignin, such as vanillin, syringaldehyd...

    Authors: Shihong Liu, Michael Gonzalez, Celine Kong, Scott Weir and Aaron M. Socha

    Citation: Biotechnology for Biofuels 2021 14:47

    Content type: Research

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  16. Biochemical conversion of lignocellulosic biomass to simple sugars at commercial scale is hampered by the high cost of saccharifying enzymes. Lytic polysaccharide monooxygenases (LPMOs) may hold the key to ove...

    Authors: Adnan Kadić, Anikó Várnai, Vincent G. H. Eijsink, Svein Jarle Horn and Gunnar Lidén

    Citation: Biotechnology for Biofuels 2021 14:46

    Content type: Research

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  17. It is interesting to modify sugar metabolic pathways to improve the productivity of biocatalysts that convert sugars to value-added products. However, this attempt often fails due to the tight control of the s...

    Authors: Toru Jojima, Takafumi Igari, Ryoji Noburyu, Akira Watanabe, Masako Suda and Masayuki Inui

    Citation: Biotechnology for Biofuels 2021 14:45

    Content type: Research

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  18. Bioconversion of wood into bioproducts and biofuels is hindered by the recalcitrance of woody raw material to bioprocesses such as enzymatic saccharification. Targeted modification of the chemical composition ...

    Authors: Madhavi Latha Gandla, Niklas Mähler, Sacha Escamez, Tomas Skotare, Ogonna Obudulu, Linus Möller, Ilka N. Abreu, Joakim Bygdell, Magnus Hertzberg, Torgeir R. Hvidsten, Thomas Moritz, Gunnar Wingsle, Johan Trygg, Hannele Tuominen and Leif J. Jönsson

    Citation: Biotechnology for Biofuels 2021 14:43

    Content type: Research

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  19. The global demand for functional proteins is extensive, diverse, and constantly increasing. Medicine, agriculture, and industrial manufacturing all rely on high-quality proteins as major active components or p...

    Authors: Jennifer A. Schmidt, Lubna V. Richter, Lisa A. Condoluci and Beth A. Ahner

    Citation: Biotechnology for Biofuels 2021 14:42

    Content type: Research

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  20. Baker’s yeast is a widely used eukaryotic cell factory, producing a diverse range of compounds including biofuels and fine chemicals. The use of lignocellulose as feedstock offers the opportunity to run these ...

    Authors: Friederike Gutmann, Cosimo Jann, Filipa Pereira, Andreas Johansson, Lars M. Steinmetz and Kiran R. Patil

    Citation: Biotechnology for Biofuels 2021 14:41

    Content type: Research

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  21. An amendment to this paper has been published and can be accessed via the original article.

    Authors: Jeffrey P. Tingley, Kristin E. Low, Xiaohui Xing and D. Wade Abbott

    Citation: Biotechnology for Biofuels 2021 14:40

    Content type: Correction

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    The original article was published in Biotechnology for Biofuels 2021 14:16

  22. Trichoderma reesei is a filamentous fungus that is important as an industrial producer of cellulases and hemicellulases due to its high secretion of these enzymes and outstanding performance in industrial ferment...

    Authors: Nozomu Shibata, Hiroshi Kakeshita, Kazuaki Igarashi, Yasushi Takimura, Yosuke Shida, Wataru Ogasawara, Tohru Koda, Tomohisa Hasunuma and Akihiko Kondo

    Citation: Biotechnology for Biofuels 2021 14:39

    Content type: Research

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  23. Saccharomyces cerevisiae is well-known as an ideal model system for basic research and important industrial microorganism for biotechnological applications. Acetic acid is an important growth inhibitor that has d...

    Authors: Yanfei Cheng, Hui Zhu, Zhengda Du, Xuena Guo, Chenyao Zhou, Zhaoyue Wang and Xiuping He

    Citation: Biotechnology for Biofuels 2021 14:38

    Content type: Research

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  24. Woody plants with high glucose content are alternative bioresources for the production of biofuels and biochemicals. Various pretreatment methods may be used to reduce the effects of retardation factors such a...

    Authors: Dae-Seok Lee, Yoon-Gyo Lee, Eun Jin Cho, Younho Song and Hyeun-Jong Bae

    Citation: Biotechnology for Biofuels 2021 14:37

    Content type: Research

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  25. Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis;...

    Authors: Lin Dai, Tian Huang, Kankan Jiang, Xin Zhou and Yong Xu

    Citation: Biotechnology for Biofuels 2021 14:35

    Content type: Research

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  26. Butanol (n-butanol) has been gaining attention as a renewable energy carrier and an alternative biofuel with superior properties to the most widely used ethanol. We performed 48 anaerobic fermentations simultaneo...

    Authors: Cansu Birgen, Kristin F. Degnes, Sidsel Markussen, Alexander Wentzel and Håvard Sletta

    Citation: Biotechnology for Biofuels 2021 14:34

    Content type: Research

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  27. Low- and high-affinity glucose transport system is a conserved strategy of microorganism to cope with environmental glucose fluctuation for their growth and competitiveness. In Neurospora crassa, the dual-affinit...

    Authors: Jinyang Li, Qian Liu, Jingen Li, Liangcai Lin, Xiaolin Li, Yongli Zhang and Chaoguang Tian

    Citation: Biotechnology for Biofuels 2021 14:33

    Content type: Research

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  28. One of the sustainable development goals focuses on the biomass-based production as a replacement for fossil-based commodities. A novel feedstock with vast potentials is tunicate biomass, which can be pretreat...

    Authors: Kateřina Hrůzová, Leonidas Matsakas, Anthi Karnaouri, Fredrik Norén, Ulrika Rova and Paul Christakopoulos

    Citation: Biotechnology for Biofuels 2021 14:32

    Content type: Research

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  29. Penicillium funiculosum NCIM1228 is a non-model filamentous fungus that produces high-quality secretome for lignocellulosic biomass saccharification. Despite having desirable traits to be an industrial workhorse,...

    Authors: Anmoldeep Randhawa, Nandita Pasari, Tulika Sinha, Mayank Gupta, Anju M. Nair, Olusola A. Ogunyewo, Sandhya Verma, Praveen Kumar Verma and Syed Shams Yazdani

    Citation: Biotechnology for Biofuels 2021 14:31

    Content type: Research

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  30. The discovery of lytic polysaccharide monooxygenases (LPMOs) has fundamentally changed our understanding of microbial lignocellulose degradation. Cellulomonas bacteria have a rich history of study due to their ab...

    Authors: James Li, Laleh Solhi, Ethan D. Goddard-Borger, Yann Mathieu, Warren W. Wakarchuk, Stephen G. Withers and Harry Brumer

    Citation: Biotechnology for Biofuels 2021 14:29

    Content type: Research

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  31. Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that catalyze oxidative depolymerization of industrially relevant crystalline polysaccharides, such as cellulose, in a reaction that depends o...

    Authors: Anton A. Stepnov, Zarah Forsberg, Morten Sørlie, Giang-Son Nguyen, Alexander Wentzel, Åsmund K. Røhr and Vincent G. H. Eijsink

    Citation: Biotechnology for Biofuels 2021 14:28

    Content type: Research

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  32. Lignin intermediates resulting from lignocellulose degradation have been suspected to hinder anaerobic mineralisation of organic materials to biogas. Phenyl acids like phenylacetate (PAA) are early detectable ...

    Authors: Eva Maria Prem, Mira Mutschlechner, Blaz Stres, Paul Illmer and Andreas Otto Wagner

    Citation: Biotechnology for Biofuels 2021 14:27

    Content type: Research

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  33. Renewable chemicals have attracted attention due to increasing interest in environmental concerns and resource utilization. Biobased production of industrial compounds from nonfood biomass has become increasin...

    Authors: Hsiang-Yen Su, Hua-Ying Li, Cai-Yun Xie, Qiang Fei and Ke-Ke Cheng

    Citation: Biotechnology for Biofuels 2021 14:26

    Content type: Research

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  34. Humic acids (HA) have been used in biorefinery process due to its surfactant properties as an aid to the pretreatment of lignocellulose, with results indicating a positive effect on delignification. However, t...

    Authors: Wei Tang, Xinxing Wu, Caoxing Huang, Zhe Ling, Chenhuan Lai and Qiang Yong

    Citation: Biotechnology for Biofuels 2021 14:25

    Content type: Research

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  35. The cellulolytic thermophile Clostridium thermocellum is an important biocatalyst due to its ability to solubilize lignocellulosic feedstocks without the need for pretreatment or exogenous enzyme addition. At low...

    Authors: Dhananjay Beri, Christopher D. Herring, Sofie Blahova, Suresh Poudel, Richard J. Giannone, Robert L. Hettich and Lee R. Lynd

    Citation: Biotechnology for Biofuels 2021 14:24

    Content type: Research

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  36. The global production of glycerol is increasing year by year since the demands of biodiesel is rising. It is benefit for high-yield succinate synthesis due to its high reducing property. A. succinogenes, a succin...

    Authors: Tianwen Zheng, Bin Xu, Yaliang Ji, Wenming Zhang, Fengxue Xin, Weiliang Dong, Ping Wei, Jiangfeng Ma and Min Jiang

    Citation: Biotechnology for Biofuels 2021 14:23

    Content type: Research

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  37. Both APETALA2/Ethylene Responsive Factor (AP2/ERF) superfamily and R2R3-MYB family were from one of the largest diverse families of transcription factors (TFs) in plants, and played important roles in plant de...

    Authors: Guanlan Xing, Jinyu Li, Wenli Li, Sin Man Lam, Hongli Yuan, Guanghou Shui and Jinshui Yang

    Citation: Biotechnology for Biofuels 2021 14:22

    Content type: Research

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  38. Predatory flagellates and ciliates are two common bio-contaminants which frequently cause biomass losses in Chlorella mass culture. Efficient and targeted ways are required to control these contaminations in Chlo...

    Authors: Xiaobin Wen, Aoqi Zhang, Xiaoyan Zhu, Lin Liang, Yan Huo, Kaixuan Wang, Youzhi Yu, Yahong Geng, Yi Ding and Yeguang Li

    Citation: Biotechnology for Biofuels 2021 14:21

    Content type: Research

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  39. Microalgae have attracted considerable interest due to their ability to produce a wide range of valuable compounds. Pulsed Electric Fields (PEF) has been demonstrated to effectively disrupt the microalgae cell...

    Authors: I. Papachristou, S. Akaberi, A. Silve, E. Navarro-López, R. Wüstner, K. Leber, N. Nazarova, G. Müller and W. Frey

    Citation: Biotechnology for Biofuels 2021 14:20

    Content type: Research

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  40. Increasing the efficiency of the biogas production process is possible by modifying the technological installations of the biogas plant. In this study, specific solutions based on a mathematical model that lea...

    Authors: Karol Postawa, Jerzy Szczygieł and Marek Kułażyński

    Citation: Biotechnology for Biofuels 2021 14:19

    Content type: Research

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  41. Biomass composition varies from plant to plant and greatly affects biomass utilization. Lignin is a heterogeneous phenolic polymer derived mainly from p-coumaryl, coniferyl, and sinapyl alcohols and makes up to 1...

    Authors: Fang Chen, Chunliu Zhuo, Xirong Xiao, Thomas H. Pendergast and Katrien M. Devos

    Citation: Biotechnology for Biofuels 2021 14:18

    Content type: Methodology

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  42. The production of biofuels as an efficient source of renewable energy has received considerable attention due to increasing energy demands and regulatory incentives to reduce greenhouse gas emissions. Second-g...

    Authors: Jeffrey P. Tingley, Kristin E. Low, Xiaohui Xing and D. Wade Abbott

    Citation: Biotechnology for Biofuels 2021 14:16

    Content type: Review

    Published on:

    The Correction to this article has been published in Biotechnology for Biofuels 2021 14:40

  43. Bacillus subtilis CS13 was previously isolated for 2,3-butanediol (2,3-BD) and poly-γ-glutamic acid (γ-PGA) co-production. When culturing this strain without L-glutamic acid in the medium, 2,3-BD is the main meta...

    Authors: Dexin Wang, Baek-Rock Oh, Sungbeom Lee, Dae-Hyuk Kim and Min-Ho Joe

    Citation: Biotechnology for Biofuels 2021 14:15

    Content type: Research

    Published on:

  44. Efficient utilization of all available carbons from lignocellulosic biomass is critical for economic efficiency of a bioconversion process to produce renewable bioproducts. However, the metabolic responses tha...

    Authors: Zhangyang Xu, Chunmei Pan, Xiaolu Li, Naijia Hao, Tong Zhang, Matthew J. Gaffrey, Yunqiao Pu, John R. Cort, Arthur J. Ragauskas, Wei-Jun Qian and Bin Yang

    Citation: Biotechnology for Biofuels 2021 14:11

    Content type: Research

    Published on: