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  1. The ability to deconstruct plant biomass without conventional pretreatment has made members of the genus Caldicellulosiruptor the target of investigation for the consolidated processing of lignocellulosic biomass...

    Authors: Sun-Ki Kim, Daehwan Chung, Michael E. Himmel, Yannick J. Bomble and Janet Westpheling
    Citation: Biotechnology for Biofuels 2016 9:176
    Content type: Research Published on:

  2. Glycerol, a byproduct of biodiesel, has become a readily available and inexpensive carbon source for the production of high-value products. However, the main drawback of glycerol utilization is the low consump...

    Authors: Ruilian Yao, Dewang Xiong, Hongbo Hu, Masataka Wakayama, Wenjuan Yu, Xuehong Zhang and Kazuyuki Shimizu
    Citation: Biotechnology for Biofuels 2016 9:175
    Content type: Research Published on:

  3. In general, biofuel production involves biomass pretreatment and enzymatic saccharification, followed by the subsequent sugar conversion to biofuel via fermentation. The crucial step in the production of biofu...

    Authors: Chia Sing Chan, Lee Li Sin, Kok-Gan Chan, Mohd Shahir Shamsir, Fazilah Abd Manan, Rajesh Kumar Sani and Kian Mau Goh
    Citation: Biotechnology for Biofuels 2016 9:174
    Content type: Research Published on:

  4. Acetic acid, released during hydrolysis of lignocellulosic feedstocks for second generation bioethanol production, inhibits yeast growth and alcoholic fermentation. Yeast biomass generated in a propagation ste...

    Authors: Daniel González-Ramos, Arthur R. Gorter de Vries, Sietske S. Grijseels, Margo C. van Berkum, Steve Swinnen, Marcel van den Broek, Elke Nevoigt, Jean-Marc G. Daran, Jack T. Pronk and Antonius J. A. van Maris
    Citation: Biotechnology for Biofuels 2016 9:173
    Content type: Research Published on:

  5. Lignocellulosic biomass is one of earth’s most abundant resources, and it has great potential for biofuel production because it is renewable and has carbon-neutral characteristics. Lignocellulose is mainly com...

    Authors: Tao Sheng, Lei Zhao, Ling-Fang Gao, Wen-Zong Liu, Min-Hua Cui, Ze-Chong Guo, Xiao-Dan Ma, Shih-Hsin Ho and Ai-Jie Wang
    Citation: Biotechnology for Biofuels 2016 9:172
    Content type: Research Published on:

  6. One of the most promising technologies to sustainably produce energy and to mitigate greenhouse gas emissions from combustion of fossil energy carriers is the anaerobic digestion and biomethanation of organic ...

    Authors: Irena Maus, Daniela E. Koeck, Katharina G. Cibis, Sarah Hahnke, Yong S. Kim, Thomas Langer, Jana Kreubel, Marcel Erhard, Andreas Bremges, Sandra Off, Yvonne Stolze, Sebastian Jaenicke, Alexander Goesmann, Alexander Sczyrba, Paul Scherer, Helmut König…
    Citation: Biotechnology for Biofuels 2016 9:171
    Content type: Research Published on:

  7. Bio-jet fuels compatible with current aviation infrastructure are needed as an alternative to petroleum-based jet fuel to lower greenhouse gas emissions and reduce dependence on fossil fuels. Cradle to grave l...

    Authors: Erik Budsberg, Jordan T. Crawford, Hannah Morgan, Wei Shan Chin, Renata Bura and Rick Gustafson
    Citation: Biotechnology for Biofuels 2016 9:170
    Content type: Research Published on:

  8. Trichoderma reesei is the principal producer of cellulolytic enzymes. Because of the strong influence on the enzyme production, the morphology of the filamentous fungi is a key paramet...

    Authors: Vera Novy, Maximilian Schmid, Manuel Eibinger, Zdenek Petrasek and Bernd Nidetzky
    Citation: Biotechnology for Biofuels 2016 9:169
    Content type: Research Published on:

  9. The genus Aspergillus includes microorganisms that naturally degrade lignocellulosic biomass, secreting large amounts of carbohydrate-active enzymes (CAZymes) that characterize their saprophyte lifestyle. Aspergi...

    Authors: Marcelo Ventura Rubio, Mariane Paludetti Zubieta, João Paulo Lourenço Franco Cairo, Felipe Calzado, Adriana Franco Paes Leme, Fabio Marcio Squina, Rolf Alexander Prade and André Ricardo de Lima Damásio
    Citation: Biotechnology for Biofuels 2016 9:168
    Content type: Research Published on:

  10. The production of ethanol and other fuels and chemicals from lignocellulosic materials is dependent of efficient xylose conversion. Xylose fermentation capacity in yeasts is usually linked to xylose reductase ...

    Authors: Raquel M. Cadete, Alejandro M. de las Heras, Anders G. Sandström, Carla Ferreira, Francisco Gírio, Marie-Françoise Gorwa-Grauslund, Carlos A. Rosa and César Fonseca
    Citation: Biotechnology for Biofuels 2016 9:167
    Content type: Research Published on:

  11. The biomass recalcitrance resulting from its chemical compositions and physical structures impedes the conversion of biomass into fermentable sugars. Pretreatment is a necessary procedure to increase the cellu...

    Authors: Han-Yin Li, Xue Chen, Chen-Zhou Wang, Shao-Ni Sun and Run-Cang Sun
    Citation: Biotechnology for Biofuels 2016 9:166
    Content type: Research Published on:

  12. Photosynthetic organisms can be used for renewable and sustainable production of fuels and high-value compounds from natural resources. Costs for design and operation of large-scale algae cultivation systems c...

    Authors: Robert J. Flassig, Melanie Fachet, Kai Höffner, Paul I. Barton and Kai Sundmacher
    Citation: Biotechnology for Biofuels 2016 9:165
    Content type: Research Published on:

  13. The concerted action of three complementary cellulases from Clostridium thermocellum, engineered to be stable at elevated temperatures, was examined on a cellulosic substrate and compared to that of the wild-type...

    Authors: Sarah MoraĂŻs, Johanna Stern, Amaranta Kahn, Anastasia P. Galanopoulou, Shahar Yoav, Melina Shamshoum, Matthew A. Smith, Dimitris G. Hatzinikolaou, Frances H. Arnold and Edward A. Bayer
    Citation: Biotechnology for Biofuels 2016 9:164
    Content type: Research Published on:

  14. Microbial electrosynthesis (MES) and gas fermentation are bioenergy technologies in which a microbial catalyst reduces CO2 into organic carbon molecules with electrons from the cathode of a bioelectrochemical sys...

    Authors: Fariza Ammam, Pier-Luc Tremblay, Dawid M. Lizak and Tian Zhang
    Citation: Biotechnology for Biofuels 2016 9:163
    Content type: Research Published on:

  15. Biohythane is a new and high-value transportation fuel present as a mixture of biomethane and biohydrogen. It has been produced from different organic matters using anaerobic digestion. Bioenergy can be recove...

    Authors: Qian Liu, Zhiyong Jason Ren, Cong Huang, Bingfeng Liu, Nanqi Ren and Defeng Xing
    Citation: Biotechnology for Biofuels 2016 9:162
    Content type: Research Published on:

  16. As a key strategy for mitigating global climate change, bioenergy production by reducing CO2 emissions plays an important role in ensuring sustainable development. However, land-use change by converting natural e...

    Authors: Wei Liu, Cheng Peng, Zhifen Chen, Yue Liu, Juan Yan, Jianqiang Li and Tao Sang
    Citation: Biotechnology for Biofuels 2016 9:161
    Content type: Research Published on:

  17. Concerns around greenhouse gas emissions necessitate the development of sustainable processes for the production of chemicals, materials, and fuels from alternative renewable sources. The lignocellulosic plant...

    Authors: Ramakrishnan Parthasarathi, Jian Sun, Tanmoy Dutta, Ning Sun, Sivakumar Pattathil, N. V. S. N. Murthy Konda, Angelo Gabriel Peralta, Blake A. Simmons and Seema Singh
    Citation: Biotechnology for Biofuels 2016 9:160
    Content type: Research Published on:

  18. Phospholipase A1 is an enzyme that hydrolyzes phospholipids at the sn-1 position. It has potential applications across diverse fields including food, pharmaceutical, and biofuel industries. Although there has bee...

    Authors: Hye Jin Lim, Yu Jin Park, Yeon Jae Jang, Ji Eun Choi, Joon Young Oh, Ji Hyun Park, Jae Kwang Song and Dong-Myung Kim
    Citation: Biotechnology for Biofuels 2016 9:159
    Content type: Research Published on:

  19. Engineering of the yeast Saccharomyces cerevisiae for improved utilization of pentose sugars is vital for cost-efficient cellulosic bioethanol production. Although endogenous hexose transporters (Hxt) can be engi...

    Authors: Jeroen G. Nijland, Erwin Vos, Hyun Yong Shin, Paul P. de Waal, Paul Klaassen and Arnold J. M. Driessen
    Citation: Biotechnology for Biofuels 2016 9:158
    Content type: Research Published on:

  20. Lignocellulosic ethanol production involves major steps such as thermochemical pretreatment of biomass, enzymatic hydrolysis of pre-treated biomass and the fermentation of released sugars into ethanol. At leas...

    Authors: Anu Jose Mattam, Arindam Kuila, Niranjan Suralikerimath, Nettem Choudary, Peddy V. C. Rao and Harshad Ravindra Velankar
    Citation: Biotechnology for Biofuels 2016 9:157
    Content type: Research Published on:

  21. Biofuel production from conversion of biomass is indispensable in the portfolio of renewable energies. Complex microbial communities are involved in the anaerobic digestion process of plant material, agricultu...

    Authors: Yvonne Stolze, Andreas Bremges, Madis Rumming, Christian Henke, Irena Maus, Alfred PĂĽhler, Alexander Sczyrba and Andreas SchlĂĽter
    Citation: Biotechnology for Biofuels 2016 9:156
    Content type: Research Published on:

  22. Methane yield and biogas productivity of biogas plants (BGPs) depend on microbial community structure and function, substrate supply, and general biogas process parameters. So far, however, relatively little i...

    Authors: R. Heyer, D. Benndorf, F. Kohrs, J. De Vrieze, N. Boon, M. Hoffmann, E. Rapp, Andreas SchlĂĽter, Alexander Sczyrba and U. Reichl
    Citation: Biotechnology for Biofuels 2016 9:155
    Content type: Research Published on:

  23. Biofuels production from plant biomasses is a complex multi-step process with important economic burdens. Several biotechnological approaches have been pursued to reduce biofuels production costs. The aim of t...

    Authors: Daniela Castiglia, Lorenza Sannino, Loredana Marcolongo, Elena Ionata, Rachele Tamburino, Angelo De Stradis, Beatrice Cobucci-Ponzano, Marco Moracci, Francesco La Cara and Nunzia Scotti
    Citation: Biotechnology for Biofuels 2016 9:154
    Content type: Research Published on:

  24. Second-generation ethanol (2G-bioethanol) uses lignocellulosic feedstocks for ethanol production. Sugarcane is one among the most suitable crops for biofuel production. Its juice is extracted for sugar product...

    Authors: Maria Thereza Bazzo Martins, Wagner Rodrigo de Souza, Bárbara Andrade Dias Brito da Cunha, Marcos Fernando Basso, Nelson Geraldo de Oliveira, Felipe Vinecky, Polyana Kelly Martins, Patrícia Abrão de Oliveira, Bruna Cersózimo Arenque-Musa, Amanda Pereira de Souza, Marcos Silveira Buckeridge, Adilson Kenji Kobayashi, Betania Ferraz Quirino and Hugo Bruno Correa Molinari
    Citation: Biotechnology for Biofuels 2016 9:153
    Content type: Research Published on:

  25. Steam explosion pretreatment has been examined in many studies for enhancing the enzymatic digestibility of lignocellulosic biomass and is currently the most common pretreatment method in commercial biorefiner...

    Authors: Thomas Pielhop, Janick Amgarten, Philipp Rudolf von Rohr and Michael H. Studer
    Citation: Biotechnology for Biofuels 2016 9:152
    Content type: Research Published on:

  26. Microalgae are emerging as suitable feedstock for renewable biofuel production and providing a promising way to alleviate green house gas CO2. Characterizing the metabolic pathways involved in the biosynthesis of...

    Authors: Huifeng Peng, Dong Wei, Gu Chen and Feng Chen
    Citation: Biotechnology for Biofuels 2016 9:151
    Content type: Research Published on:

  27. Intermediates from processing sugar beets are considered an attractive feedstock for ethanol fermentation due to their high fermentable sugar content. In particular, medium prepared from raw sugar beet juice s...

    Authors: Piotr Dziugan, Maria Balcerek, Michal J. Binczarski, Dorota Kregiel, Marcin Kucner, Alina Kunicka-Styczynska, Katarzyna Pielech-Przybylska, Krzysztof Smigielski and Izabela A. Witonska
    Citation: Biotechnology for Biofuels 2016 9:150
    Content type: Research Published on:

  28. The oleaginous yeast, Yarrowia lipolytica, has been utilized as an industrial host for about 60 years for various applications. Recently, the metabolic engineering of this host has become increasingly popular due...

    Authors: Gabriel M. Rodriguez, Murtaza Shabbir Hussain, Lauren Gambill, Difeng Gao, Allison Yaguchi and Mark Blenner
    Citation: Biotechnology for Biofuels 2016 9:149
    Content type: Research Published on:

  29. Global climate change and fossil fuels limitations have boosted the demand for robust and efficient microbial factories for the manufacturing of bio-based products from renewable feedstocks. In this regard, ef...

    Authors: Jasper Sloothaak, Juan Antonio Tamayo-Ramos, Dorett I. Odoni, Thanaporn Laothanachareon, Christian Derntl, Astrid R. Mach-Aigner, Vitor A. P. Martins dos Santos and Peter J. Schaap
    Citation: Biotechnology for Biofuels 2016 9:148
    Content type: Research Published on:

  30. β-Glucosidase is an important member of the biomass-degrading enzyme system, and plays vital roles in enzymatic saccharification for biofuels production. Candidates with high activity and great stability over ...

    Authors: Wei Xia, Xinxin Xu, Lichun Qian, Pengjun Shi, Yingguo Bai, Huiying Luo, Rui Ma and Bin Yao
    Citation: Biotechnology for Biofuels 2016 9:147
    Content type: Research Published on:

  31. Butanol is not only an important solvent and chemical intermediate in food and pharmaceutical industries, but also considered as an advanced biofuel. Recently, there have been resurging interests in producing ...

    Authors: Chuang Xue, Zixuan Wang, Shudong Wang, Xiaotong Zhang, Lijie Chen, Ying Mu and Fengwu Bai
    Citation: Biotechnology for Biofuels 2016 9:146
    Content type: Research Published on:

  32. Sugarcane is one of the world’s most profitable crops. Waste steam-exploded sugarcane bagasse (SEB) is a cheap, abundant, and renewable lignocellulosic feedstock for the next-generation biofuels. In nature, fu...

    Authors: Neil Andrew Brown, Laure N. A. Ries, Thaila F. Reis, Ranjith Rajendran, Renato Augusto Corrêa dos Santos, Gordon Ramage, Diego Mauricio Riaño-Pachón and Gustavo H. Goldman
    Citation: Biotechnology for Biofuels 2016 9:145
    Content type: Research Published on:

  33. During strategic planning of a biogas plant, the local availability of resources for start-up and operation should be taken into consideration for a cost-efficient process. Because most bioethanol/sugar indust...

    Authors: Athaydes Francisco Leite, Leandro Janke, Hauke Harms, Hans-Hermann Richnow and Marcell Nikolausz
    Citation: Biotechnology for Biofuels 2016 9:144
    Content type: Research Published on:

  34. Sugarcane, a tropical C4 perennial crop, is capable of producing 30–100 tons or more of biomass per hectare annually. The lignocellulosic residue remaining after sugar extraction is currently underutilized and...

    Authors: Charleson R. Poovaiah, William P. Bewg, Wu Lan, John Ralph and Heather D. Coleman
    Citation: Biotechnology for Biofuels 2016 9:143
    Content type: Research Published on:

  35. The requirement for efficient and green conversion technologies has prompted hydrothermal processing as a promising treatment option for sustainable biorefinery industry. The treatment has been applied to proc...

    Authors: Zhi-Wen Wang, Ming-Qiang Zhu, Ming-Fei Li, Jun-Qi Wang, Qin Wei and Run-Cang Sun
    Citation: Biotechnology for Biofuels 2016 9:142
    Content type: Research Published on:

  36. Conceptually, multi-functional enzymes are attractive because in the case of complex polymer hydrolysis having two or more activities defined by a single enzyme offers the possibility of synergy and reduced en...

    Authors: Hanen Bouraoui, Marie-Laure Desrousseaux, Eleni Ioannou, Pablo Alvira, Mohamed Manaï, Caroline Rémond, Claire Dumon, Narcis Fernandez-Fuentes and Michael J. O’Donohue
    Citation: Biotechnology for Biofuels 2016 9:140
    Content type: Research Published on:

  37. Caffeoyl shikimate esterase (CSE) was recently characterized as an enzyme central to the lignin biosynthetic pathway in Arabidopsis thaliana. The cse-2 loss-of-function mutant shows a typical phenotype of lignin-...

    Authors: LĂ­via Vargas, Igor Cesarino, Ruben Vanholme, Wannes Voorend, Marina de Lyra Soriano Saleme, Kris Morreel and Wout Boerjan
    Citation: Biotechnology for Biofuels 2016 9:139
    Content type: Research Published on:

  38. Energy shortage has become a global problem. Production of biofuels from renewable biomass resources is an inevitable trend of sustainable development. Cellulose is the most abundant and renewable resource in ...

    Authors: Chao Yang, Yu Xia, Hong Qu, An-Dong Li, Ruihua Liu, Yubo Wang and Tong Zhang
    Citation: Biotechnology for Biofuels 2016 9:138
    Content type: Research Published on:

  39. Consolidated bioprocessing (CBP), integrating cellulase production, cellulose saccharification, and fermentation into one step has been widely considered as the ultimate low-cost configuration for producing se...

    Authors: Li-Hai Fan, Zi-Jian Zhang, Sen Mei, Yang-Yang Lu, Mei Li, Zai-Yu Wang, Jian-Guo Yang, Shang-Tian Yang and Tian-Wei Tan
    Citation: Biotechnology for Biofuels 2016 9:137
    Content type: Research Published on:

  40. Camelina sativa is an emerging dedicated oilseed crop designed for biofuel and biodiesel applications as well as a source for edible and general-purpose oils. Such valuable oilseed cro...

    Authors: Hesham M. Abdullah, Parisa Akbari, Bibin Paulose, Danny Schnell, Weipeng Qi, Yeonhwa Park, Ashwani Pareek and Om Parkash Dhankher
    Citation: Biotechnology for Biofuels 2016 9:136
    Content type: Research Published on:

  41. In order to establish a cost-efficient biodiesel biorefinery, valorization of its main by-product, crude glycerol, is imperative. Recently, Ustilago trichophora TZ1 was found to efficiently produce malic acid fro...

    Authors: Thiemo Zambanini, Wiebke Kleineberg, Eda Sarikaya, Joerg M. Buescher, Guido Meurer, Nick Wierckx and Lars M. Blank
    Citation: Biotechnology for Biofuels 2016 9:135
    Content type: Research Published on:

  42. Poly-Îł-glutamic acid (Îł-PGA) is a naturally occurring biopolymer made from repeating units of l-glutamic acid, d-glutamic acid, or both. Since some bacteria are capable of vigorous Îł-PGA biosynthesis from renewab...

    Authors: Zhiting Luo, Yuan Guo, Jidong Liu, Hua Qiu, Mouming Zhao, Wei Zou and Shubo Li
    Citation: Biotechnology for Biofuels 2016 9:134
    Content type: Review Published on:

  43. Glycoside hydrolases (GH) targeting cellulose, xylan, and chitin are common in the bacterial genomes that have been sequenced. Little is known, however, about the architecture of multi-domain and multi-activit...

    Authors: Darrian Talamantes, Nazmehr Biabini, Hoang Dang, Kenza Abdoun and Renaud Berlemont
    Citation: Biotechnology for Biofuels 2016 9:133
    Content type: Research Published on:

  44. The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina) is a widely used industrial host organism for protein production. In industrial cultivations, it can produce over 100 g/l of extracellular ...

    Authors: Tiina M. Pakula, Heli Nygren, Dorothee Barth, Markus Heinonen, Sandra Castillo, Merja Penttilä and Mikko Arvas
    Citation: Biotechnology for Biofuels 2016 9:132
    Content type: Research Published on:

  46. Infrastructure compatible hydrocarbon biofuel proposed to qualify as renewable transportation fuel under the U.S. Energy Independence and Security Act of 2007 and Renewable Fuel Standard (RFS2) is evaluated. T...

    Authors: Jordan T. Crawford, Chin Wei Shan, Erik Budsberg, Hannah Morgan, Renata Bura and Rick Gustafson
    Citation: Biotechnology for Biofuels 2016 9:141
    Content type: Research Published on:

  47. Industrial biotechnology that is able to provide environmentally friendly bio-based products has attracted more attention in replacing petroleum-based industries. Currently, most of the carbon sources used for...

    Authors: Qiang Fei, Marykate O’Brien, Robert Nelson, Xiaowen Chen, Andrew Lowell and Nancy Dowe
    Citation: Biotechnology for Biofuels 2016 9:130
    Content type: Research Published on:

  48. C5–C8 medium-chain carboxylic acids are valuable chemicals as the precursors of various chemicals and transport fuels. However, only a few strict anaerobes have been discovered to produce them and their produc...

    Authors: Byoung Seung Jeon, Okkyoung Choi, Youngsoon Um and Byoung-In Sang
    Citation: Biotechnology for Biofuels 2016 9:129
    Content type: Research Published on:

  49. Materials rich in lignocellulose, such as straw, are abundant, cheap and highly interesting for biogas production. However, the complex structure of lignocellulose is difficult for microbial cellulolytic enzym...

    Authors: Li Sun, Tong Liu, Bettina MĂĽller and Anna SchnĂĽrer
    Citation: Biotechnology for Biofuels 2016 9:128
    Content type: Research Published on:

  50. SWEET is a newly identified family of sugar transporters. Although SWEET transporters have been characterized by using Arabidopsis and rice, very little knowledge of sucrose accumulation in the stem region is ...

    Authors: Hiroshi Mizuno, Shigemitsu Kasuga and Hiroyuki Kawahigashi
    Citation: Biotechnology for Biofuels 2016 9:127
    Content type: Research Published on:
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