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  1. It is necessary to develop efficient methods to produce renewable fuels from lignocellulosic biomass. One of the main challenges to the industrialization of lignocellulose conversion processes is the large amo...

    Authors: Noah Weiss, Johan Börjesson, Lars Saaby Pedersen and Anne S Meyer

    Citation: Biotechnology for Biofuels 2013 6:5

    Content type: Research

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  2. Diminishing supplies of fossil fuels and oil spills are rousing to explore the alternative sources of energy that can be produced from non-food/feed-based substrates. Due to its abundance, sugarcane bagasse (S...

    Authors: Anuj K Chandel, Felipe F A Antunes, Virgilio Anjos, Maria J V Bell, Leonarde N Rodrigues, Om V Singh, Carlos A Rosa, Fernando C Pagnocca and Silvio S da Silva

    Citation: Biotechnology for Biofuels 2013 6:4

    Content type: Research

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  3. A solid-state anaerobic digestion method is used to produce biogas from various solid wastes in China but the efficiency of methane production requires constant improvement. The diversity and abundance of rele...

    Authors: An Li, Ya’nan Chu, Xumin Wang, Lufeng Ren, Jun Yu, Xiaoling Liu, Jianbin Yan, Lei Zhang, Shuangxiu Wu and Shizhong Li

    Citation: Biotechnology for Biofuels 2013 6:3

    Content type: Research

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  4. While simultaneous saccharification and co-fermentation (SSCF) is considered to be a promising process for bioconversion of lignocellulosic materials to ethanol, there are still relatively little demo-plant da...

    Authors: Rakesh Koppram, Fredrik Nielsen, Eva Albers, Annika Lambert, Sune Wännström, Lars Welin, Guido Zacchi and Lisbeth Olsson

    Citation: Biotechnology for Biofuels 2013 6:2

    Content type: Research

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  5. Lignin materials are abundant and among the most important potential sources for biofuel production. Development of an efficient lignin degradation process has considerable potential for the production of a va...

    Authors: Yan Shi, Liyuan Chai, Chongjian Tang, Zhihui Yang, Huan Zhang, Runhua Chen, Yuehui Chen and Yu Zheng

    Citation: Biotechnology for Biofuels 2013 6:1

    Content type: Research

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  6. Fermentation production of biofuel ethanol consumes agricultural crops, which will compete directly with the food supply. As an alternative, photosynthetic cyanobacteria have been proposed as microbial factori...

    Authors: Jiangxin Wang, Lei Chen, Siqiang Huang, Jie Liu, Xiaoyue Ren, Xiaoxu Tian, Jianjun Qiao and Weiwen Zhang

    Citation: Biotechnology for Biofuels 2012 5:89

    Content type: Research

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  7. Acetoin and 2,3-butanediol are two important biorefinery platform chemicals. They are currently fermented below 40°C using mesophilic strains, but the processes often suffer from bacterial contamination.

    Authors: Zijun Xiao, Xiangming Wang, Yunling Huang, Fangfang Huo, Xiankun Zhu, Lijun Xi and Jian R Lu

    Citation: Biotechnology for Biofuels 2012 5:88

    Content type: Research

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  8. Corn cob residue (CCR) is a kind of waste lignocellulosic material with enormous potential for bioethanol production. The moderated sulphite processes were used to enhance the hydrophily of the material by sul...

    Authors: Lingxi Bu, Yang Xing, Hailong Yu, Yuxia Gao and Jianxin Jiang

    Citation: Biotechnology for Biofuels 2012 5:87

    Content type: Research

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  9. The economic and environmental viability of dedicated terrestrial energy crops is in doubt. The production of large scale biomass (macroalgae) for biofuels in the marine environment was first tested in the lat...

    Authors: Adam D Hughes, Maeve S Kelly, Kenneth D Black and Michele S Stanley

    Citation: Biotechnology for Biofuels 2012 5:86

    Content type: Review

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  10. The thermophilic anaerobe Thermoanaerobacterium saccharolyticum is capable of directly fermenting xylan and the biomass-derived sugars glucose, cellobiose, xylose, mannose, galactose and arabinose. It has been me...

    Authors: Vasiliki Tsakraklides, A Joe Shaw, Bethany B Miller, David A Hogsett and Christopher D Herring

    Citation: Biotechnology for Biofuels 2012 5:85

    Content type: Research

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  11. Cost-efficient generation of second-generation biofuels requires plant biomass that can easily be degraded into sugars and further fermented into fuels. However, lignocellulosic biomass is inherently recalcitr...

    Authors: Pia Damm Petersen, Jane Lau, Berit Ebert, Fan Yang, Yves Verhertbruggen, Jin Sun Kim, Patanjali Varanasi, Anongpat Suttangkakul, Manfred Auer, Dominique Loqué and Henrik Vibe Scheller

    Citation: Biotechnology for Biofuels 2012 5:84

    Content type: Research

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  12. The recalcitrance of lignocellulosic cell wall biomass to deconstruction varies greatly in angiosperms, yet the source of this variation remains unclear. Here, in eight genotypes of short rotation coppice will...

    Authors: Nicholas JB Brereton, Michael J Ray, Ian Shield, Peter Martin, Angela Karp and Richard J Murphy

    Citation: Biotechnology for Biofuels 2012 5:83

    Content type: Research

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  13. A modified laboratory-scale upflow anaerobic sludge blanket (UASB) reactor was used to obtain methane by treating hydrous ethanol vinasse. Vinasses or stillage are waste materials with high organic loads, and ...

    Authors: Elda I España-Gamboa, Javier O Mijangos-Cortés, Galdy Hernández-Zárate, Jorge A Domínguez Maldonado and Liliana M Alzate-Gaviria

    Citation: Biotechnology for Biofuels 2012 5:82

    Content type: Research

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  14. The inherent recalcitrance of lignocellulosic biomass is one of the major economic hurdles for the production of fuels and chemicals from biomass. Additionally, lignin is recognized as having a negative impact...

    Authors: Kelsey L Yee, Miguel Rodriguez Jr, Timothy J Tschaplinski, Nancy L Engle, Madhavi Z Martin, Chunxiang Fu, Zeng-Yu Wang, Scott D Hamilton-Brehm and Jonathan R Mielenz

    Citation: Biotechnology for Biofuels 2012 5:81

    Content type: Research

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  15. For lignocellulosic bioenergy to become a viable alternative to traditional energy production methods, rapid increases in conversion efficiency and biomass yield must be achieved. Increased productivity in bio...

    Authors: Frank Alex Feltus and Joshua P Vandenbrink

    Citation: Biotechnology for Biofuels 2012 5:80

    Content type: Review

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  16. Recent studies demonstrate that enzymes from the glycosyl hydrolase family 61 (GH61) show lytic polysaccharide monooxygenase (PMO) activity. Together with cellobiose dehydrogenase (CDH) an enzymatic system cap...

    Authors: Roman Kittl, Daniel Kracher, Daniel Burgstaller, Dietmar Haltrich and Roland Ludwig

    Citation: Biotechnology for Biofuels 2012 5:79

    Content type: Research

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  17. Microorganisms employ a multiplicity of enzymes to efficiently degrade the composite structure of plant cell wall cellulosic polysaccharides. These remarkable enzyme systems include glycoside hydrolases (cellu...

    Authors: Sarah Moraïs, Yoav Barak, Raphael Lamed, David B Wilson, Qi Xu, Michael E Himmel and Edward A Bayer

    Citation: Biotechnology for Biofuels 2012 5:78

    Content type: Research

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  18. Thioesterases remove the fatty acyl moiety from the fatty acyl-acyl carrier proteins (ACPs), releasing them as free fatty acids (FFAs), which can be further used to produce a variety of fatty acid-based biofue...

    Authors: Yanning Zheng, Lingling Li, Qiang Liu, Wen Qin, Jianming Yang, Yujin Cao, Xinglin Jiang, Guang Zhao and Mo Xian

    Citation: Biotechnology for Biofuels 2012 5:76

    Content type: Research

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  19. High tolerance to ethanol is a desirable characteristics for ethanologenic strains used in industrial ethanol fermentation. A deeper understanding of the molecular mechanisms underlying ethanologenic strains t...

    Authors: Ming-xiong He, Bo Wu, Zong-xia Shui, Qi-chun Hu, Wen-guo Wang, Fu-rong Tan, Xiao-yu Tang, Qi-li Zhu, Ke Pan, Qing Li and Xiao-hong Su

    Citation: Biotechnology for Biofuels 2012 5:75

    Content type: Research

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  20. The lack of sequenced genomes for oleaginous microalgae limits our understanding of the mechanisms these organisms utilize to become enriched in triglycerides. Here we report the de novo transcriptome assembly an...

    Authors: Hamid Rismani-Yazdi, Berat Z Haznedaroglu, Carol Hsin and Jordan Peccia

    Citation: Biotechnology for Biofuels 2012 5:74

    Content type: Research

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  21. A complete saccharification of plant polymers is the critical step in the efficient production of bio-alcohols. Beta-glucosidases acting in the degradation of intermediate gluco-oligosaccharides produced by ce...

    Authors: Mercedes V Del Pozo, Lucía Fernández-Arrojo, Jorge Gil-Martínez, Alejandro Montesinos, Tatyana N Chernikova, Taras Y Nechitaylo, Agnes Waliszek, Marta Tortajada, Antonia Rojas, Sharon A Huws, Olga V Golyshina, Charles J Newbold, Julio Polaina, Manuel Ferrer and Peter N Golyshin

    Citation: Biotechnology for Biofuels 2012 5:73

    Content type: Research

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  22. Down-regulation of the caffeic acid 3-O-methyltransferase EC 2.1.1.68 (COMT) gene in the lignin biosynthetic pathway of switchgrass (Panicum virgatum) resulted in cell walls of transgenic plants releasing more co...

    Authors: Timothy J Tschaplinski, Robert F Standaert, Nancy L Engle, Madhavi Z Martin, Amandeep K Sangha, Jerry M Parks, Jeremy C Smith, Reichel Samuel, Nan Jiang, Yunqiao Pu, Arthur J Ragauskas, Choo Y Hamilton, Chunxiang Fu, Zeng-Yu Wang, Brian H Davison, Richard A Dixon…

    Citation: Biotechnology for Biofuels 2012 5:71

    Content type: Research

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  23. Due to a growing world population and increasing welfare, energy demand worldwide is increasing. To meet the increasing energy demand in a sustainable way, new technologies are needed. The Plant-Microbial Fuel...

    Authors: Marjolein Helder, David PBTB Strik, Hubertus VM Hamelers and Cees JN Buisman

    Citation: Biotechnology for Biofuels 2012 5:70

    Content type: Research

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  24. Our companion paper discussed the yield benefits achieved by integrating deacetylation, mechanical refining, and washing with low acid and low temperature pretreatment. To evaluate the impact of the modified p...

    Authors: Ling Tao, Xiaowen Chen, Andy Aden, Eric Kuhn, Michael E Himmel, Melvin Tucker, Mary Ann A Franden, Min Zhang, David K Johnson, Nancy Dowe and Richard T Elander

    Citation: Biotechnology for Biofuels 2012 5:69

    Content type: Research

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  25. Recent studies have demonstrated that photosynthetic cyanobacteria could be an excellent cell factory to produce renewable biofuels and chemicals due to their capability to utilize solar energy and CO2 as the sol...

    Authors: Jie Liu, Lei Chen, Jiangxin Wang, Jianjun Qiao and Weiwen Zhang

    Citation: Biotechnology for Biofuels 2012 5:68

    Content type: Review

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  26. In the present study, three ionic liquids, namely 1-butyl-3-methylimidazolium chloride ([BMIM]Cl), 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc), and 1-ethyl-3-methylimidazolium diethyl phosphate ([EMIM]DEP)...

    Authors: Teck Nam Ang, Gek Cheng Ngoh, Adeline Seak May Chua and Min Gyu Lee

    Citation: Biotechnology for Biofuels 2012 5:67

    Content type: Research

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  27. The branched chain alcohol isobutanol exhibits superior physicochemical properties as an alternative biofuel. The yeast Saccharomyces cerevisiae naturally produces low amounts of isobutanol as a by-product during...

    Authors: Dawid Brat, Christian Weber, Wolfram Lorenzen, Helge B Bode and Eckhard Boles

    Citation: Biotechnology for Biofuels 2012 5:65

    Content type: Research

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  28. While the ethanol production from biomass by consolidated bioprocess (CBP) is considered to be the most ideal process, simultaneous saccharification and fermentation (SSF) is the most appropriate strategy in p...

    Authors: Enoch Y Park, Kazuya Naruse and Tatsuya Kato

    Citation: Biotechnology for Biofuels 2012 5:64

    Content type: Research

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  29. The enzymatic conversion of lignocellulosic plant biomass into fermentable sugars is a crucial step in the sustainable and environmentally friendly production of biofuels. However, a major drawback of enzymes ...

    Authors: Holger Klose, Juliane Röder, Michele Girfoglio, Rainer Fischer and Ulrich Commandeur

    Citation: Biotechnology for Biofuels 2012 5:63

    Content type: Research

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  30. Effective pretreatment is key to achieving high enzymatic saccharification efficiency in processing lignocellulosic biomass to fermentable sugars, biofuels and value-added products. Ionic liquids (ILs), still ...

    Authors: Sergios Kimon Karatzos, Leslie Alan Edye and William Orlando Sinclair Doherty

    Citation: Biotechnology for Biofuels 2012 5:62

    Content type: Research

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  31. Very high gravity (VHG) fermentation using medium in excess of 250 g/L sugars for more than 15% (v) ethanol can save energy consumption, not only for ethanol distillation, but also for distillage treatment; ho...

    Authors: Chen-Guang Liu, Na Wang, Yen-Han Lin and Feng-Wu Bai

    Citation: Biotechnology for Biofuels 2012 5:61

    Content type: Research

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  32. Historically, acid pretreatment technology for the production of bio-ethanol from corn stover has required severe conditions to overcome biomass recalcitrance. However, the high usage of acid and steam at seve...

    Authors: Xiaowen Chen, Ling Tao, Joseph Shekiro, Ali Mohaghaghi, Steve Decker, Wei Wang, Holly Smith, Sunkyu Park, Michael E Himmel and Melvin Tucker

    Citation: Biotechnology for Biofuels 2012 5:60

    Content type: Research

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  33. Lignin is an integral component of the plant cell wall matrix but impedes the conversion of biomass into biofuels. The plasticity of lignin biosynthesis should permit the inclusion of new compatible phenolic m...

    Authors: Sasikumar Elumalai, Yuki Tobimatsu, John H Grabber, Xuejun Pan and John Ralph

    Citation: Biotechnology for Biofuels 2012 5:59

    Content type: Research

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  34. Lignocellulose is the most abundant biomass on earth. However, biomass recalcitrance has become a major factor affecting biofuel production. Although cellulose crystallinity significantly influences biomass sa...

    Authors: Ning Xu, Wei Zhang, Shuangfeng Ren, Fei Liu, Chunqiao Zhao, Haofeng Liao, Zhengdan Xu, Jiangfeng Huang, Qing Li, Yuanyuan Tu, Bin Yu, Yanting Wang, Jianxiong Jiang, Jingping Qin and Liangcai Peng

    Citation: Biotechnology for Biofuels 2012 5:58

    Content type: Research

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  35. A common trend in the research on 2nd generation bioethanol is the focus on intensifying the process and increasing the concentration of water insoluble solids (WIS) throughout the process. However, increasing th...

    Authors: Benny Palmqvist and Gunnar Lidén

    Citation: Biotechnology for Biofuels 2012 5:57

    Content type: Research

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  36. Because many Miscanthus genotypes can be cultivated with relatively high productivity and carbohydrate content, Miscanthus has great potential as an energy crop that can support large scale biological production ...

    Authors: Taiying Zhang, Charles E Wyman, Katrin Jakob and Bin Yang

    Citation: Biotechnology for Biofuels 2012 5:56

    Content type: Research

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  37. Degradation of cellulose to glucose requires the cooperative action of three classes of enzymes, collectively known as cellulases. Endoglucanases randomly bind to cellulose surfaces and generate new chain ends...

    Authors: Andrea Asztalos, Marcus Daniels, Anurag Sethi, Tongye Shen, Paul Langan, Antonio Redondo and Sandrasegaram Gnanakaran

    Citation: Biotechnology for Biofuels 2012 5:55

    Content type: Research

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  38. Thermophilic fungi have attracted increased interest for their ability to secrete enzymes that deconstruct biomass at high temperatures. However, development of thermophilic fungi as enzyme producers for bioma...

    Authors: Shara D McClendon, Tanveer Batth, Christopher J Petzold, Paul D Adams, Blake A Simmons and Steven W Singer

    Citation: Biotechnology for Biofuels 2012 5:54

    Content type: Research

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  39. To achieve an economical cellulosic ethanol production, a host that can do both cellulosic saccharification and ethanol fermentation is desirable. However, to engineer a non-cellulolytic yeast to be such a hos...

    Authors: Jui-Jen Chang, Cheng-Yu Ho, Feng-Ju Ho, Tsung-Yu Tsai, Huei-Mien Ke, Christine H-T Wang, Hsin-Liang Chen, Ming-Che Shih, Chieh-Chen Huang and Wen-Hsiung Li

    Citation: Biotechnology for Biofuels 2012 5:53

    Content type: Research

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  40. Fungi are important players in the turnover of plant biomass because they produce a broad range of degradative enzymes. Aspergillus nidulans, a well-studied saprophyte and close homologue to industrially importan...

    Authors: Sayali Saykhedkar, Anamika Ray, Patricia Ayoubi-Canaan, Steven D Hartson, Rolf Prade and Andrew J Mort

    Citation: Biotechnology for Biofuels 2012 5:52

    Content type: Research

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  41. Cellulose amorphogenesis, described as the non-hydrolytic “opening up” or disruption of a cellulosic substrate, is becoming increasingly recognized as one of the key steps in the enzymatic deconstruction of ce...

    Authors: Keith Gourlay, Valdeir Arantes and Jack N Saddler

    Citation: Biotechnology for Biofuels 2012 5:51

    Content type: Methodology

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  42. Lipids produced from filamentous fungi show great promise for biofuel production, but a major limiting factor is the high production cost attributed to feedstock. Lignocellulosic biomass is a suitable feedstoc...

    Authors: Yubin Zheng, Xiaochen Yu, Jijiao Zeng and Shulin Chen

    Citation: Biotechnology for Biofuels 2012 5:50

    Content type: Research

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  43. The impact of hydrothermal flowthrough (FT) pretreatment severity on pretreatment and solubilization performance metrics was evaluated for three milled feedstocks (corn stover, bagasse, and poplar) and two con...

    Authors: Veronique Archambault-Leger, Xiongjun Shao and Lee R Lynd

    Citation: Biotechnology for Biofuels 2012 5:49

    Content type: Research

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  44. The considerable increase in biodiesel production worldwide in the last 5 years resulted in a stoichiometric increased coproduction of crude glycerol. As an excess of crude glycerol has been produced, its valu...

    Authors: João R M Almeida, Léia C L Fávaro and Betania F Quirino

    Citation: Biotechnology for Biofuels 2012 5:48

    Content type: Review

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  45. Temporal and spatial expression of fatty acid and lipid biosynthetic genes are associated with the accumulation of storage lipids in the seeds of oil plants. In jatropha (Jatropha curcas L.), a potential biofuel ...

    Authors: Keyu Gu, Chengxin Yi, Dongsheng Tian, Jatinder Singh Sangha, Yan Hong and Zhongchao Yin

    Citation: Biotechnology for Biofuels 2012 5:47

    Content type: Research

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  46. The bulk terrestrial biomass resource in a future bio-economy will be lignocellulosic biomass, which is recalcitrant and challenging to process. Enzymatic conversion of polysaccharides in the lignocellulosic b...

    Authors: Svein Jarle Horn, Gustav Vaaje-Kolstad, Bjørge Westereng and VincentGH Eijsink

    Citation: Biotechnology for Biofuels 2012 5:45

    Content type: Review

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