Combining directed evolution of pathway enzymes and dynamic pathway regulation using a quorum-sensing circuit to improve the production of 4-hydroxyphenylacetic acid in Escherichia coli

Background 4-Hydroxyphenylacetic acid (4HPAA) is an important building block for synthesizing drugs, agrochemicals, biochemicals, etc. 4HPAA is currently produced exclusively via petrochemical processes and the process is environmentally unfriendly and unsustainable. Microbial cell factory would be an attractive approach for 4HPAA production. Results In the present study, we established a microbial biosynthetic system for the de novo production of 4HPAA from glucose in Escherichia coli. First, we compared different biosynthetic pathways for the production of 4HPAA. The yeast Ehrlich pathway produced the highest level of 4HPAA among these pathways that were evaluated. To increase the pathway efficiency, the yeast Ehrlich pathway enzymes were directedly evolved via error-prone PCR. Two phenylpyruvate decarboxylase ARO10 and phenylacetaldehyde dehydrogenase FeaB variants that outperformed the wild-type enzymes were obtained. These mutations increased the in vitro and in vivo catalytic efficiency for converting 4-hydroxyphenylpyruvate to 4HPAA. A tunable intergenic region (TIGR) sequence was inserted into the two evolved genes to balance their expression. Regulation of TIGR for the evolved pathway enzymes further improved the production of 4HPAA, resulting in a 1.13-fold increase in titer compared with the fusion wild-type pathway. To prevent the toxicity of a heterologous pathway to the cell, an Esa quorum-sensing (QS) circuit with both activating and repressing functions was developed for inducer-free productions of metabolites. The Esa-PesaR activation QS system was used to dynamically control the biosynthetic pathway of 4HPAA in E. coli, which achieved 17.39 ± 0.26 g/L with a molar yield of 23.2% without addition of external inducers, resulting in a 46.4% improvement of the titer compared to the statically controlled pathway. Conclusion We have constructed an E. coli for 4HPAA production with the highest titer to date. This study also demonstrates that the combination of directed evolution of pathway enzymes and dynamic pathway regulation using a QS circuit is a powerful strategy of metabolic engineering for the productions of metabolites. Electronic supplementary material The online version of this article (10.1186/s13068-019-1438-3) contains supplementary material, which is available to authorized users.


Background
Phenolic acids are aromatic acids that contain a phenol ring and at least one organic carboxylic acid group. Phenolic acids play an important role in human health and have wide applications in food, cosmetic and pharmaceutical industries. 4-Hydroxyphenylacetic acid (4HPAA) has received much attention because of its numerous applications. 4HPAA is used in the synthesis of penicillin G, atenolol, benzoprofen, and agrochemicals, etc. [1,2]. 4HPAA is an active component of Rhodiola rosea [2] and the Chinese herbs Aster tataricus (fan hun cao). Aster tataricus is widely used in China for the treatment of pneumonia, HBV, and carcinomas [3][4][5]. Furthermore, 4HPAA possesses anxiolytic [6], antiplatelet [7] and hepatoprotective [8] properties. In addition, 4HPAA was considered as a potential hypopigmenting agent [9] and an inhibitor of hypertonicity and hypoxia [10].
4HPAA can be obtained by chemical synthesis from different substrates such as anisol, p-cresol, phenol, benzyl phenyl ether, or hydroxymandelic acid [11,12]. However, the chemical routes have some drawbacks, including a requirement of elevated temperatures and pressures for the reaction, and the use of expensive solvents. Thus, biotechnological approach has been explored as an alternative. A biotransformation reaction using nitrilase has been used for 4HPAA production [2]. Koma et al. [1] engineered an Escherichia coli for the production of 4HPAA from glucose. Overexpression of the indole-3-pyruvate/phenylpyruvate decarboxylase gene ipdC from Azospirillum brasilense NBRC102289, and the phenylacetaldehyde dehydrogenase gene feaB from E. coli in a tyrosine overproducing E. coli strain resulted in the production of 6.1 mM (0.93 g/L) 4HPAA with a yield of 13.2% (mol/mol) in a shake flask culture. However, the titer of 4HPAA in engineered microorganisms is much lower than that of other aromatic compounds [13,14]. Thus, further work is required to increase the production of 4HPAA.
Quorum-sensing (QS) systems has been considered as an auto-induction system that is regulated by cell density. The Lux and Esa QS systems are the main QS systems that have been reported to date. Recently, an Esa QS system from Pantoea stewartii has been engineered to automatically downregulate the competing pathway, significantly improving the production of myo-inositol, glucaric acid and shikimic acid [15].
In this study, we first compared different biosynthetic pathways of 4HPAA (Fig. 1). Then, the biosynthetic pathway enzymes of 4HPAA were directedly evolved and mediated using a tunable intergenic region (TIGR) sequence. Finally, the TIGR-mediated biosynthetic pathway was dynamically regulated using a quorum-sensing circuit. The resulting E. coli produced 17.39 ± 0.26 g/L 4HPAA without addition of external inducers, which is the highest value reported to date.

Strains, plasmids and primers
The bacterial strains, plasmids, and primers used in this study are listed in Table 1. E. coli DH5α was used for gene cloning in this study. The L-DOPA overproducing strain E. coli DOPA-30N [16] was used as the host strain for the production of 4HPAA.

Generation of random mutagenesis libraries using error-prone PCR and screening
MEGAWHOP-PCR [22] was performed for the construction of libraries. The random mutagenesis libraries of the fusion ARO10-feaB gene cluster were constructed through error-prone PCR. Primer pairs AROF/FeaR were used for amplification the ARO10-feaB gene cluster using pBbB2k-ARO10-L-feaB as a template. The PCR reaction mixture (50 μL) consisted of 5 mM MgCl 2 , 0.3 mM MnCl 2 , 0.2 mM each of dATP, dGTP, dCTP and dTTP, and 2.5 U of rTaq DNA polymerase. The PCR products were then used as megaprimers to perform MEGAWHOP-PCR using pBbB2k-ARO10-L-feaB as a template. Following the MEGAWHOP-PCR, DpnI digestion (20 U) of the surplus template in the PCR reaction mixture was performed at 37 °C overnight, then DpnI was inactivated at 80 °C for 20 min. The PCR products were transformed into E. coli DH5a cells, plated on LB agar, and then pooled together after overnight growth to create a liquid library.
The mutant plasmid was recovered from the library and transferred into the L-DOPA overproducer E. coli DOPA-30N. Then, the resulting transformants were plated on LB agar with 50 mg/mL kanamycin and 120 ng/mL anhydrotetracycline. After overnight growth, individual colonies were inoculated into a 48-well deep-well microplate (4.6 mL) containing 1 mL of the fermentation medium and then incubated at 37 °C and 1000 rpm for 72 h on a MBR-420FL shaker (TAITEC Corporation, Saitamaken, Japan). Because L-DOPA can be easily oxidized to dopachrome and then polymerized non-enzymatically to form melanin, the culture broth of the host strain E. coli DOPA-30N became black [16]. Because 4HPAA shares the same precursor (tyrosine) with L-DOPA, light color cultures were selected for further shake flask analysis of 4HPAA production.
TIGRs were synthesized using PCR to assemble the oligonucleotides into chimeric DNA sequences as described by Pfleger et al. [23] and Li et al. [19]. The assembled products were purified using a nucleotide removal column, amplified using the end-specific primers TIGRs-F(X)/TIGRs-R(A), and then cloned into the NheI/NotI sites of pBbB2k-ARO10*-feaB* to obtain the plasmid libraries pBbB2k-ARO10*-TIGRs-feaB*. The plasmid libraries were transferred into component DOPA-30N to generate the mutant library.
The TIGR library was plated on LB agar plates containing 50 μg/mL kanamycin and 120 ng/mL anhydrotetracycline. The plates were then incubated at 30 °C overnight. Single colonies were inoculated into a 48-well deep-well microplate (4.6 mL) containing 1 mL of fermentation medium, and then incubated at 30 °C and 1000 rpm for

Construction of a quorum-sensing circuit
The quorum-sensing fragment containing the signal molecule acyl-homoserine lactone (AHL) synthase gene esaI, the transcriptional regulator esaR I70V , and P easS and P esaR promoters was synthesized by GENEWIZ, Inc (Suzhou, China) and then cloned into the MluI/SalI of pZBK [19] to obtain the plasmid pZBK-P easS IC-P easR AS (Additional file 1: Figure S1A). The TIGR-mediated gene cluster of the evolved ARO10 and feaB was cloned  into the multiple cloning site 2 (MCS2) of pZBK-P easS IC-P easR AS to obtain pZBK-P esaR AS-4HPAA.

Production of 4HPAA
A single colony was inoculated into 5 mL of LB medium in a falcon tube then cultured overnight at 30 °C. The overnight seed culture was then inoculated into 50 mL of fermentation medium with a starting OD 600 of 0.1. The fermentation medium contained (per liter) tryptone (10 g), yeast extract (5 g), NaCl (10 g Fed-batch fermentation was carried out in a 2 L bioreactor (MiniBox 2L*2 Parallel Bioreactor System, T&J Bio-engineering (Shanghai) Co. LTD, Shanghai, China) containing 1.2 L of fermentation medium with an initial OD 600 of approximately 0.1. Fermentation was carried out at 30 °C with an airflow of 1.2 L/min and a starting agitation rate of 400 rpm. For E. coli containing the statically controlled pathway, 120 ng/mL anhydrotetracycline was added as an inducer after 6 h. The pH was controlled at 7.0 by automatic addition of NH 4 OH. Dissolved oxygen was maintained above 25% by adjusting the agitation rate. The feed solution (pH 7.0,) contained 500 g/L glucose and 30 g/L MgSO 4 ·7H 2 O. The feed was introduced continuously into the fermenter using a pH-stat feeding strategy. Once the glucose was exhausted, the pH rose rapidly. When the pH was greater than 7.0 by 0.1 of a pH unit, the feed was automatically added to the fermenter. Samples were periodically withdrawn, and the following parameters were measured: OD 600 , residual glucose concentrations, and 4HPAA concentrations. Fermentation experiments were performed in duplicate.

Catalytic efficiency assays
For in vitro activity assay, E. coli DOPA-30N harboring pBbB2k-ARO10-L-feaB or pBbB2k-ARO10*-L-feaB* were pre-inoculated in 5 mL of LB medium overnight and then inoculated into 50 mL the fermentation medium containing kanamycin. The cultures were incubated at 30 °C and 200 rpm until OD 600 reached 2.5 and then induced with 120 ng/mL anhydrotetracycline. After 44 h, the culture broth was disrupted directly using an Ultrahigh Pressure Cell Disrupter (JN-3000Plus, Guangzhou Juneng Nano & Bio Technology Co. LTD, China), and then centrifuged to remove cell debris. The supernatant was selected as a crude enzyme solution for catalytic efficiency assay. The total protein concentration in the crude extract was determined by a Bradford assay using a Bio-Rad protein assay kit (Bio-Rad Laboratories, Hercules, CA, USA). The catalytic efficiency assay was conducted as follows: 4-hydroxyphenylpyruvic acid of 10 mM was added to the crude enzyme. The reaction was conducted at 37 °C for 6 h. Then, the reaction mixture was immediately placed on ice broth to stop the reaction. The product (4HPAA) concentration in the reaction solution was determined immediately by HPLC.
For in vivo activity assay, E. coli BW25113 was transformed with pBbB2k-ARO10-L-feaB and pBbB2k-ARO10*-L-feaB*, respectively. Single colonies were pre-inoculated into 5 mL of LB medium containing kanamycin and cultured overnight at 30 °C and 200 rpm. A 500 μL overnight cultures were inoculated into 50 mL the fermentation medium containing kanamycin. The cultures were incubated at 30 °C and 200 rpm until OD 600 reached 2.5 and then induced with 120 ng/mL anhydrotetracycline at 37 °C and 200 rpm. Three hours after induction, 4-hydroxyphenylpyruvic acid was added to a final concentration of 1.0 g/L and incubated at 30 °C and 200 rpm for 6 h. Growth and the concentration of 4HPAA were analyzed.

Assay
Growth was monitored by measuring the optical density at 600 nm. 4HPAA in the supernatants was analyzed using a Shimadzu HPLC system (LC-20A, Shimadzu, Japan) equipped with an Inertsil ODS-SP column (5 μm, 4.6 × 150 mm, GL Sciences Inc., Tokyo, Japan). The mobile phase was 0.2% TFA in methanol, and the flow rate was 0.5 mL/min at 30 °C. The methanol concentration was increased from 14 to 45% for 20 min, and then decreased to 14%. This concentration was then maintained for 10 min. A photodiode array detector (SPD-M20A) operating at 222 nm was used, and a standard curve was constructed from serial dilutions of a standard stock solution. Glucose concentrations were determined using glucose oxidase and a glucose assay kit (Shanghai Rongsheng Biotech Corporation, Shanghai, China).

Statistical analysis
All experiments were conducted in triplicate, and the data were averaged and presented as the mean ± standard deviation. One-way analysis of variance followed by Tukey's test was used to determine significant differences using the OriginPro (version 7.5) package. Statistical significance was defined as p < 0.05.

Screening biosynthetic pathways
Three biosynthetic pathways (Fig. 1) have been developed for the production of tyrosol; namely, the Ehrlich pathway via the ipdC [1] and the yeast ARO10 [24,25], the aas (encoding aromatic aldehyde synthase) pathway [26] and the tydc-tyo (encoding tyrosine decarboxylase and tyramine oxidase, respectively) pathway [27]. Because 4HPAA shares the same precursor, 4-hydroxyphenylacetaldehyde (4HPAAL), with tyrosol, we first constructed these pathways and evaluated their 4HPAA productions (Fig. 1). These pathways were then introduced into the L-DOPA overproducing E. coli strain DOPA-30N for shake flask analysis of 4HPAA. No L-DOPA was detected in the four engineered strains, indicating that, the rate of 4HPAA production was higher than that for L-DOPA (data not shown). Thus, E. coli DOPA-30N was used as the parent strain for the production of 4HPAA in this study. The introduction of all pathways resulted in the production of 4HPAA ( Table 2), indicating that these pathways can be used for 4HPAA production. Moreover, the yeast Ehrlich pathway produced the highest titer of 4HPAA ( Table 2). The recombinant strain harboring the yeast Ehrlich pathway produced 3.08 ± 0.05 g/L 4HPAA with a molar yield of 13.4%. The same result was obtained for tyrosol. The yeast Ehrlich pathway also exhibited the highest biosynthetic efficiency for tyrosol production among the four pathways [1,[24][25][26][27]. Therefore, the yeast Ehrlich pathway was selected as the biosynthetic pathway for the production of 4HPAA in this study.
Although only ipdC Ehrlich pathway was reported for the production of 4HPAA in literatures, our results demonstrated that three other pathways based on the reported tyrosol biosynthetic pathways can be used for the production of 4HPAA. Moreover, the 4HPAA titer that was obtained via our yeast Ehrlich pathway was 1.33-fold as high as that obtained via the ipdC Ehrlich pathway.

Directed evolution of the biosynthetic pathway
Independent of the enzyme structure and catalytic mechanism, directed evolution of enzyme is widely used to alter the coding sequence and improve the catalytic activities of enzymes. Thus, to increase the efficiency of the biosynthetic pathway, the fusion gene cluster of ARO10-feaB was directedly co-evolved by error-prone PCR. L-DOPA can be easily oxidized to dopachrome and then polymerized non-enzymatically to form the black pigment melanin [28]. Because the preparation of 4HPAA utilizes the same precursors (4-hydroxyphenylpyruvate/ tyrosine) as L-DOPA (Fig. 1), 4HPAA biosynthesis competes with L-DOPA. The higher the activity of the fusion gene cluster, the lower the production of L-DOPA, hence, the culture broth becomes lighter. A total of 792 colonies were used for the assay of 4HPAA production in deepwell microplate cultures. Twenty-three colonies with lighter colors were selected for further shake flask analysis. As shown in Fig. 2a, strains no. 12 and 23 produced the highest level of 4HPP (4.66 ± 0.14 and 4.65 ± 0.10 g/L, respectively). These mutant plasmids, denoted as 2E1 and 4F3, were isolated and sent for sequencing. The sequencing results are presented in Additional file 1: Table S1. In plasmid 2E1, one base mutant (C1869T) in ARO10 and three base mutants (T72G, T1195C, and A1491T) were observed in feaB. In plasmid 4F3, two base mutants (A1682G and A1842G) in ARO10 and three base mutants (C165G, A609T, and T1296) were observed in feaB. None of the base mutants in ARO10 produced an amino acid change. One amino acid mutant of feaB was observed in plasmid 2E1 (I24M) and 4F3 (N55K).
Because no amino acid mutant in ARO10 was observed after the co-evolution of the ARO10-feaB, the ARO10 in pBbB2k-ARO10-L-feaB was single-evolved using error-prone PCR. Of 900 colonies used for the assay in deep-well microplate cultures, twelve colonies with lighter colors were selected for further shake flask analysis. As shown in Fig. 2b, strains no. 6, 11, 13, and 15 produced higher levels of 4HPAA than the wildtype strain. The mutant plasmids, denoted as 6D5, 9F5, 10A3, and 10G5, were isolated and sent for sequencing. DNA sequencing revealed some base mutations and amino acid mutations in ARO10 (Additional file 1: Table S1). Two amino acid mutations (F138L and D218G) were observed in plasmid 6D5, and one mutation (V451I) was observed in plasmid 10A3.
To investigate the effects of these mutated genes on the production of 4HPAA, we combined these mutated genes to create a biosynthetic pathway and introduced the pathway into E. coli DOPA-30N. Figure 3 shows that mutations of two genes indeed increased the production of 4HPAA. The engineered strain harboring both mutated genes produced higher levels of 4HPAA than the strain that contained only one mutated gene. The combination of the mutated ARO10 from plasmid 6D5 with the mutated feaB from plasmid 2E1 resulted in the highest titer of 4HPAA (5.64 ± 0.06 g/L). This combination resulted in approximately a 90% increase in the production of 4HPAA compared to the wild-type genes.
To evaluate the applicability of the evolved pathway enzymes in microbial production of 4HPAA, we first performed in vitro crude extract enzyme assays to measure their catalytic activities. 4-Hydroxyphenylpyruvic acid of 10 mM was added into the cell crude extracts of E. coli DOPA-30N harboring the wild-type or mutant plasmid, respectively. The catalytic efficiencies were measured by testing the 4HPAA formation rate. As shown in Fig. 4, directed evolution resulted in an increase in the in vitro catalytic efficiency of the pathway to 45.55 ± 2.43 mg/h/g protein from 23.67 ± 2.66 mg/h/g protein (Fig. 4), indicating that the evolved pathway was more efficient at converting 4-hydroxyphenylpyruvate to 4HPAA than the wild-type pathway in vitro.
Then, the initial in vivo efficiency of converting 4-hydroxyphenylpyruvic acid to 4HPAA was analyzed through whole-cell bioconversion experiments. The results were compared in Fig. 4. The in vivo efficiency of the evolved pathway was 3.28 ± 0.12 mg/L/h/ OD, while the in vivo efficiency of the parent pathway was 1.91 ± 0.05 mg/L/h/OD, indicating a 72% increment in activity level. This result is consistent with the in vitro crude enzyme assays. This result indicates that the evolved pathway was more efficient at converting 4-hydroxyphenylpyruvate to 4HPAA than the wild-type pathway both in vitro and in vivo.
Directed evolution is the most commonly used approach used to significantly improve the performance of pathway enzymes. Applying direction evolution, we isolated mutant variants with improved activity than the wild-type enzymes. The best combination of the ARO10 and FeaB mutants resulted in a 90% increase in the production of 4HPAA. The increased 4HPAA titer may be as a result of increases in the activity of pathway enzymes.
The unbalanced expression of multiple genes may result in the accumulation of toxic metabolic intermediates, thereby reducing product titers. Thus, Pfleger et al. [23] developed a combinatorial engineering approach for coordinating the expression of cascade enzymes by generating libraries of TIGRs. They applied the TIGR approach to balance gene expression in the MEV pathway, resulting in a sevenfold increase in the production of mevalonate. We also applied the TIGR approach to improve the production of zeaxanthin [19] and pinene [29]. Thus, we constructed a library of TIGRs to balance the expression of the evolved ARO10* and feaB*. The library of TIGRs was inserted between the two genes. A total of 1080 colonies were used for the assay of 4HPAA production in deep-well microplate cultures. Twenty colonies with lighter colors were selected for further shake flask analysis. As shown in Fig. 5a, strains nos. 4, 10, and 12 produced higher levels of 4HPAA. We again assayed the production of 4HPAA in the three strains (Fig. 5b). Strain no. 12 produced higher levels of 4HPAA, 6.58 g/L (Fig. 5b). Regulation of TIGR resulted in a 16.7% increase in the production of 4HPAA compared with the fusion evolved pathway. The TIGR in strain no. 12 was sequenced and the results are presented in Additional file 1: Table S2.

Dynamic regulation of the biosynthetic pathway
In the above study, the inducible expression system was used to regulate the biosynthetic pathway of 4HPAA. However, this system has some disadvantages, including leaky expression, lack of dynamic control, and the prohibitively high cost of inducers that are associated with large-scale production. QS functions to control gene expression in response to cell density, and has been applied to several biosynthetic pathways to produce glucaric acid, myo-inositol, shikimate [15], β-lactamase exoenzyme [30], isopropanol [31], and bisabolene [32] in E. coli, and para-hydroxybenzoic acid in Saccharomyces cerevisiae [33]. The Lux and Esa QS systems are the main QS systems that have been reported to date. The QS system requires two regulatory elements, a signaling molecule (also known as an autoinducer) that is constitutively produced, and a receptor protein that binds the autoinducer and acts as a transcriptional activator or repressor. The Esa QS system from P. stewartii, has been engineered for both transcriptional activation and repression by regulating EsaR binding sites on the promoter E. coli DOPA-30N (pBbB2k-ARO10*-L-feaB*) was set the control. The data represent the means of three replicates, and error bars represent standard deviations [34]. In the absence of signal molecules, 3-oxohexanoylhomoserine lactone (AHL), the transcriptional regulator in EsaRI70V, binds the P esaS or P esaR promoter and activates or represses transcription, respectively [15,34,35]. Thus, we constructed an Esa QS plasmid, pZBK-P esaS IC-P esaR AS, with both activating and repressing functions (Additional file 1: Figure S1A). To characterize the function of the QS circuit, GFP and mCherry genes were successively inserted into the multiple cloning site 1 (MSC1) and MSC2 to obtain pZBK-P esaS -GFP-P esaR -mCherry (Additional file 1: Figure S1B). The relative fluorescence of GFP decreased with cell growth (Additional file 1: Figure S1C). From Additional file 1: Figure S1C, we can also see that the relative fluorescence of mCherry increased with cell growth. These results demonstrate that the P esaS -controlled gene was indeed repressed and that the P esaR -controlled gene was activated by the induction of AHL in response to cell density. Thus, the TIGR-mediated gene cluster of the evolved ARO10 6D5 and feaB 2E1 was cloned into the MCS2 site of the Esa QS plasmid pZBK-P esaS IC-P esaR AS to obtain pZBK-P esaR AS-4HPAA, which was then transferred into E. coli DOPA-30N. In this strain, the pathway was controlled by the P esaR promoter, indicating that the expression of the pathway was activated by the induction of AHL in response to cell density (Fig. 6a). To compare the production of 4HPAA between the recombinant strain harboring the QS-controlled and the statically controlled pathway, fed-batch cultures were conducted in 2-L bioreactors. The results are presented in Fig. 6b. The engineered strain harboring the statically controlled pathway produced 4HPAA of 11.88 ± 0.82 g/L with a molar yield of 14.1% at 84 h. The engineered strain harboring the QS-controlled pathway produced higher level of 4HPAA titer, which achieved 17.39 ± 0.26 g/L with a molar yield of 23.2% (mol/mol) at 76 h. This indicates that QS-regulation increased the production of 4HPAA by 46.4% compared to the statically controlled pathway. A Lux QS system from Vibrio fischeri has been used to construct a toggle switch for redirecting metabolic flux from the TCA cycle to the production of isopropanol, resulting in approximately a threefold increase in the titer [31]. This Lux QS system still requires external IPTG inducer to turn on LuxR/LuxI expression. Recently, an inducer-free Lux QS system has been used to dynamically regulate heterologous mevalonate and bisabolene biosynthetic pathways to improve the inducer-free production of bisabolene by 44% in E. coli [32]. An EsaR-P esaS QS system from P. stewartii has been constructed to improve glucaric acid in E. coli by dynamically repressing glycolysis, leading to a fivefold increase in its titer [15]. In their study, the QS system was used to repress the competing pathway, driving the flux toward the biosynthetic pathway. In our study, we constructed an Esa-P esaR activation QS system to dynamically control the biosynthetic pathway for the inducer-free production of 4HPAA, leading to a 46.4% improvement in the titer. This is the first report on an Esa activation QS system for dynamically regulating a biosynthetic pathway. The 4HPAA titer obtained in this study is 18.7-fold the maximum reported in literatures (0.93 g/L).

Conclusion
We designed three biosynthetic pathways of 4HPAA based on tyrosol synthesis. Of these pathways, the yeast Ehrlich pathway exhibited the highest efficiency for the production of 4HPAA. To increase the b Fig. 6 a The quorum-sensing (QS) system for 4HPAA production.
In the absence of 3-oxohexanoyl-homoserine lactone (AHL, red triangle), the transcriptional regulator EsaR I70V binds the P esaR promoter and represses the transcription of the pathway enzymes. EsaI proteins synthesizes autoinducer AHL, which can diffuse in or out cells and binds to the transcriptional regulator EsaR I70V . At the threshold level of AHL, which accumulates in proportion to cell density, the transcriptional regulator EsaR I70V binds to AHL, leading to dissociation of EsaR I70V from the P esaR promoter, and the EsaR I70V -AHL complex activates the transcription of the pathway enzymes. pathway efficiency, the yeast Ehrlich pathway enzymes were directedly evolved using error-prone PCR. After directed evolution, two ARO10 and FeaB variants that outperformed the wild-type enzymes were obtained. These mutations increased the efficiency for converting 4-hydroxyphenylpyruvate to 4HPAA both in vitro and in vivo. The TIGR regulation for the evolved pathway enzymes further improved 4HPAA production. An Esa QS circuit with both activating and repressing functions was developed. The Esa-P esaR activation QS system was used to dynamically control the biosynthetic pathway of 4HPAA, which yielded 17.39 ± 0.26 g/L 4HPAA without addition of external inducers. The titer is the highest value that has been obtained to date.

Additional file
Additional file 1: Figure S1. (A) Esa quorum-sensing (QS) plasmid. (B) Activation and repression of genes using quorum-sensing circuit. Table S1. Mutations on the evolved gene. Table S2. The sequence of the TIGR.

Authors' contributions
YPS performed all of the experimental works. LSF and ZBY performed the fed-batch fermentation and the assay. JZL designed the study and wrote the manuscript. All authors read and approved the final manuscript.