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Table 1 Cellulase Assays

From: Measurement of saccharifying cellulase

Enzyme

Substrate

Product measured

Cellobiase

  

   β Glucosidase

Cellobiose

Glucose

 

Cellodextrins

 
 

Salicin

Salingenin

 

p-Nitro β glucosidase

p Nitrophenol

Endo β 1, 4 glucanase

  

   Cx

Carboxymethyl cellulose

Loss in viscosity

   CMC'ase

Amorphous cellulose

Reducing sugar

 

Walseth

 
 

Sweco

 
 

Cellodextrins

 

Exo β 1, 4 Glucanase

  

   A Glucocellulase

Amorphous cellulose

Glucose (A)

 

Walseth

 

   B Cellobiohydrolase

Crystalline cellulose

Cellobiose (B)

   CBH

Avicel

 

   C1

Cellodextrins

 

Cellulase

  

   C1 + Cx

Crystalline cellulose

Loss in weight

   Avicellase

   Avicel

Reducing sugar

   Hydrocellulase

   Hydrocellulose

Reduction in optical density (OD)

   FP'ase

   Filter paper

 
 

Cellodextrins

 
 

Cotton

 

Miscellaneous

  

   Swelling factor

Cotton

Uptake of alkali

   Filter paper cellulase

Filter paper

Maceration [1]

 

Thread

Breaking strength

 

Dyed cellulose

Release of dye

  1. CMC = carboxymethyl cellulose; FP = filter paper.