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  1. Research

    Integrated analysis of transcriptome and metabolites reveals an essential role of metabolic flux in starch accumulation under nitrogen starvation in duckweed

    Duckweed is considered a promising source of energy due to its high starch content and rapid growth rate. Starch accumulation in duckweed involves complex processes that depend on the balanced expression of ge...

    Changjiang Yu, Xiaowen Zhao, Guang Qi, Zetao Bai, Yu Wang, Shumin Wang, Yubin Ma, Qian Liu, Ruibo Hu and Gongke Zhou

    Biotechnology for Biofuels 2017 10:167

    Published on: 26 June 2017

  2. Research

    A design–build–test cycle using modeling and experiments reveals interdependencies between upper glycolysis and xylose uptake in recombinant S. cerevisiae and improves predictive capabilities of large-scale kinetic models

    Recent advancements in omics measurement technologies have led to an ever-increasing amount of available experimental data that necessitate systems-oriented methodologies for efficient and systematic integrati...

    Ljubisa Miskovic, Susanne Alff-Tuomala, Keng Cher Soh, Dorothee Barth, Laura Salusjärvi, Juha-Pekka Pitkänen, Laura Ruohonen, Merja Penttilä and Vassily Hatzimanikatis

    Biotechnology for Biofuels 2017 10:166

    Published on: 26 June 2017

  3. Research

    5-Aminolevulinic acid promotes arachidonic acid biosynthesis in the red microalga Porphyridium purpureum

    The microalga Porphyridium purpureum within Rhodophyta abundantly produces several valuable proteins, polysaccharides, pigments and long-chain polyunsaturated fatty acid; it is especially effective in accumulatin...

    Kailin Jiao, Jingyu Chang, Xianhai Zeng, I-Son Ng, Zongyuan Xiao, Yong Sun, Xing Tang and Lu Lin

    Biotechnology for Biofuels 2017 10:168

    Published on: 26 June 2017

  4. Research

    Stimulation and inhibition of enzymatic hydrolysis by organosolv lignins as determined by zeta potential and hydrophobicity

    Lignin typically inhibits enzymatic hydrolysis of cellulosic biomass, but certain organosolv lignins or lignosulfonates enhance enzymatic hydrolysis. The hydrophobic and electrostatic interactions between lign...

    Yang Huang, Shaolong Sun, Chen Huang, Qiang Yong, Thomas Elder and Maobing Tu

    Biotechnology for Biofuels 2017 10:162

    Published on: 24 June 2017

  5. Research

    CRISPR–Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus

    The thermotolerant yeast Kluyveromyces marxianus shows promise as an industrial host for the biochemical production of fuels and chemicals. Wild-type strains are known to ferment high titers of ethanol and can ef...

    Ann-Kathrin Löbs, Ronja Engel, Cory Schwartz, Andrew Flores and Ian Wheeldon

    Biotechnology for Biofuels 2017 10:164

    Published on: 24 June 2017

  6. Research

    Differential β-glucosidase expression as a function of carbon source availability in Talaromyces amestolkiae: a genomic and proteomic approach

    Genomic and proteomic analysis are potent tools for metabolic characterization of microorganisms. Although cellulose usually triggers cellulase production in cellulolytic fungi, the secretion of the different ...

    Laura I. de Eugenio, Juan A. Méndez-Líter, Manuel Nieto-Domínguez, Lola Alonso, Jesús Gil-Muñoz, Jorge Barriuso, Alicia Prieto and María Jesús Martínez

    Biotechnology for Biofuels 2017 10:161

    Published on: 23 June 2017

  7. Research

    Diversity of microbial carbohydrate-active enzymes in Danish anaerobic digesters fed with wastewater treatment sludge

    Improved carbohydrate-active enzymes (CAZymes) are needed to fulfill the goal of producing food, feed, fuel, chemicals, and materials from biomass. Little is known about how the diverse microbial communities i...

    Casper Wilkens, Peter Kamp Busk, Bo Pilgaard, Wen-Jing Zhang, Kåre L. Nielsen, Per Halkjær Nielsen and Lene Lange

    Biotechnology for Biofuels 2017 10:158

    Published on: 21 June 2017

  8. Research

    A comparison of various lignin-extraction methods to enhance the accessibility and ease of enzymatic hydrolysis of the cellulosic component of steam-pretreated poplar

    Current single-stage delignification-pretreatment technologies to overcome lignocellulosic biomass recalcitrance are usually achieved at the expense of compromising the recovery of the polysaccharide component...

    Dong Tian, Richard P. Chandra, Jin-Suk Lee, Canhui Lu and Jack N. Saddler

    Biotechnology for Biofuels 2017 10:157

    Published on: 19 June 2017

  9. Research

    Management of enzyme diversity in high-performance cellulolytic cocktails

    Modern biorefineries require enzymatic cocktails of improved efficiency to generate fermentable sugars from lignocellulosic biomass. Cellulolytic fungi, among other microorganisms, have demonstrated the highes...

    Francisco Manuel Reyes-Sosa, Macarena López Morales, Ana Isabel Platero Gómez, Noelia Valbuena Crespo, Laura Sánchez Zamorano, Javier Rocha-Martín, Fernando P. Molina-Heredia and Bruno Díez García

    Biotechnology for Biofuels 2017 10:156

    Published on: 19 June 2017

  10. Research

    Comparative metabolic profiling of the lipid-producing green microalga Chlorella reveals that nitrogen and carbon metabolic pathways contribute to lipid metabolism

    Microalgae are a promising feedstock for biofuel production. Microalgal metabolic pathways are heavily influenced by environmental factors. For instance, lipid metabolism can be induced by nitrogen-limiting co...

    Hui Chen, Yanli Zheng, Jiao Zhan, Chenliu He and Qiang Wang

    Biotechnology for Biofuels 2017 10:153

    Published on: 15 June 2017

  11. Research

    Efficient dehydration and recovery of ionic liquid after lignocellulosic processing using pervaporation

    Biomass pretreatment using certain ionic liquids (ILs) is very efficient, generally producing a substrate that is amenable to saccharification with fermentable sugar yields approaching theoretical limits. Alth...

    Jian Sun, Jian Shi, N. V. S. N. Murthy Konda, Dan Campos, Dajiang Liu, Stuart Nemser, Julia Shamshina, Tanmoy Dutta, Paula Berton, Gabriela Gurau, Robin D. Rogers, Blake A. Simmons and Seema Singh

    Biotechnology for Biofuels 2017 10:154

    Published on: 15 June 2017

  12. Research

    The transcription factor PDR-1 is a multi-functional regulator and key component of pectin deconstruction and catabolism in Neurospora crassa

    Pectin is an abundant component in many fruit and vegetable wastes and could therefore be an excellent resource for biorefinery, but is currently underutilized. Fungal pectinases already play a crucial role fo...

    Nils Thieme, Vincent W. Wu, Axel Dietschmann, Asaf A. Salamov, Mei Wang, Jenifer Johnson, Vasanth R. Singan, Igor V. Grigoriev, N. Louise Glass, Chris R. Somerville and J. Philipp Benz

    Biotechnology for Biofuels 2017 10:149

    Published on: 12 June 2017

  13. Research

    Thermodynamics and economic feasibility of acetone production from syngas using the thermophilic production host Moorella thermoacetica

    Syngas fermentation is a promising option for the production of biocommodities due to its abundance and compatibility with anaerobic fermentation. Using thermophilic production strains in a syngas fermentation...

    Stephanie Redl, Sumesh Sukumara, Tom Ploeger, Liang Wu, Torbjørn Ølshøj Jensen, Alex Toftgaard Nielsen and Henk Noorman

    Biotechnology for Biofuels 2017 10:150

    Published on: 12 June 2017

  14. Research

    Proximity ligation scaffolding and comparison of two Trichoderma reesei strains genomes

    The presence of low complexity and repeated regions in genomes often results in difficulties to assemble sequencing data into full chromosomes. However, the availability of full genome scaffolds is essential t...

    Etienne Jourdier, Lyam Baudry, Dante Poggi-Parodi, Yoan Vicq, Romain Koszul, Antoine Margeot, Martial Marbouty and Frédérique Bidard

    Biotechnology for Biofuels 2017 10:151

    Published on: 12 June 2017

  15. Review

    Regulators of plant biomass degradation in ascomycetous fungi

    Fungi play a major role in the global carbon cycle because of their ability to utilize plant biomass (polysaccharides, proteins, and lignin) as carbon source. Due to the complexity and heterogenic composition ...

    Tiziano Benocci, Maria Victoria Aguilar-Pontes, Miaomiao Zhou, Bernhard Seiboth and Ronald P. de Vries

    Biotechnology for Biofuels 2017 10:152

    Published on: 12 June 2017

  16. Research

    The advanced strategy for enhancing biobutanol production and high-efficient product recovery with reduced wastewater generation

    Butanol as an important chemical and potential fuel could be produced via ABE fermentation from lignocellulosic biomass. The use of food-related feedstocks such as maize and sugar cane may not be a sustainable...

    Chuang Xue, Xiaotong Zhang, Jufang Wang, Min Xiao, Lijie Chen and Fengwu Bai

    Biotechnology for Biofuels 2017 10:148

    Published on: 10 June 2017

  17. Research

    Combined “de novo” and “ex novo” lipid fermentation in a mix-medium of corncob acid hydrolysate and soybean oil by Trichosporon dermatis

    Microbial oil is one important bio-product for its important function in energy, chemical, and food industry. Finding suitable substrates is one key issue for its industrial application. Both hydrophilic and h...

    Chao Huang, Mu-Tan Luo, Xue-Fang Chen, Gao-Xiang Qi, Lian Xiong, Xiao-Qing Lin, Can Wang, Hai-Long Li and Xin-De Chen

    Biotechnology for Biofuels 2017 10:147

    Published on: 9 June 2017

  18. Review

    Sweet sorghum as biofuel feedstock: recent advances and available resources

    Sweet sorghum is a promising target for biofuel production. It is a C4 crop with low input requirements and accumulates high levels of sugars in its stalks. However, large-scale planting on marginal lands woul...

    Supriya Mathur, A. V. Umakanth, V. A. Tonapi, Rita Sharma and Manoj K. Sharma

    Biotechnology for Biofuels 2017 10:146

    Published on: 8 June 2017

  19. Research

    Can we use short rotation coppice poplar for sugar based biorefinery feedstock? Bioconversion of 2-year-old poplar grown as short rotation coppice

    Feedstock cost is a substantial barrier to the commercialization of lignocellulosic biorefineries. Poplar grown using a short rotation coppice (SRC) system has the potential to provide a low-cost feedstock and...

    Chang Dou, Wilian F. Marcondes, Jessica E. Djaja, Renata Bura and Rick Gustafson

    Biotechnology for Biofuels 2017 10:144

    Published on: 5 June 2017

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