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  1. Research

    Expression of Aspergillus niger CAZymes is determined by compositional changes in wheat straw generated by hydrothermal or ionic liquid pretreatments

    The capacity of fungi, such as Aspergillus niger, to degrade lignocellulose is harnessed in biotechnology to generate biofuels and high-value compounds from renewable feedstocks. Most feedstocks are currently pre...

    Paul Daly, Jolanda M. van Munster, Martin J. Blythe, Roger Ibbett, Matt Kokolski, Sanyasi Gaddipati, Erika Lindquist, Vasanth R. Singan, Kerrie W. Barry, Anna Lipzen, Chew Yee Ngan, Christopher J. Petzold, Leanne Jade G. Chan, Steven T. Pullan, Stéphane Delmas, Paul R. Waldron…

    Biotechnology for Biofuels 2017 10:35

    Published on: 7 February 2017

  2. Research

    A versatile 2A peptide-based bicistronic protein expressing platform for the industrial cellulase producing fungus, Trichoderma reesei

    The industrial workhorse fungus, Trichoderma reesei, is typically exploited for its ability to produce cellulase enzymes, whereas use of this fungus for over-expression of other proteins (homologous and heterolog...

    Venkataramanan Subramanian, Logan A. Schuster, Kyle T. Moore, Larry E. Taylor, John O. Baker, Todd A. Vander Wall, Jeffrey G. Linger, Michael E. Himmel and Stephen R. Decker

    Biotechnology for Biofuels 2017 10:34

    Published on: 6 February 2017

  3. Research

    Development of a genetically programed vanillin-sensing bacterium for high-throughput screening of lignin-degrading enzyme libraries

    Lignin is a potential biorefinery feedstock for the production of value-added chemicals including vanillin. A huge amount of lignin is produced as a by-product of the paper industry, while cellulosic componen...

    Barindra Sana, Kuan Hui Burton Chia, Sarada S. Raghavan, Balamurugan Ramalingam, Niranjan Nagarajan, Jayasree Seayad and Farid J. Ghadessy

    Biotechnology for Biofuels 2017 10:32

    Published on: 3 February 2017

  4. Research

    Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling

    Glycoside hydrolases (GHs) are enzymes that hydrolyze polysaccharides into simple sugars. To better understand the specificity of enzyme hydrolysis within the complex matrix of polysaccharides found in the pla...

    Johnnie A. Walker, Sivakumar Pattathil, Lai F. Bergeman, Emily T. Beebe, Kai Deng, Maryam Mirzai, Trent R. Northen, Michael G. Hahn and Brian G. Fox

    Biotechnology for Biofuels 2017 10:31

    Published on: 2 February 2017

  5. Research

    Development of a low-cost cellulase production process using Trichoderma reesei for Brazilian biorefineries

    During the past few years, the first industrial-scale cellulosic ethanol plants have been inaugurated. Although the performance of the commercial cellulase enzymes used in this process has greatly improved ove...

    Simo Ellilä, Lucas Fonseca, Cristiane Uchima, Junio Cota, Gustavo Henrique Goldman, Markku Saloheimo, Vera Sacon and Matti Siika-aho

    Biotechnology for Biofuels 2017 10:30

    Published on: 2 February 2017

  6. Research

    Coupling and uncoupling of triglyceride and beta-carotene production by Dunaliella salina under nitrogen limitation and starvation

    Nitrogen starvation and limitation are known to induce important physiological changes especially in lipid metabolism of microalgae (triglycerides, membrane lipids, beta-carotene, etc.). Although little inform...

    Hubert Bonnefond, Nina Moelants, Amélie Talec, Patrick Mayzaud, Olivier Bernard and Antoine Sciandra

    Biotechnology for Biofuels 2017 10:25

    Published on: 31 January 2017

  7. Methodology

    Efficient estimation of the maximum metabolic productivity of batch systems

    Production of chemicals from engineered organisms in a batch culture involves an inherent trade-off between productivity, yield, and titer. Existing strategies for strain design typically focus on designing mu...

    Peter C. St. John, Michael F. Crowley and Yannick J. Bomble

    Biotechnology for Biofuels 2017 10:28

    Published on: 31 January 2017

  8. Research

    A framework for accelerated phototrophic bioprocess development: integration of parallelized microscale cultivation, laboratory automation and Kriging-assisted experimental design

    Even though microalgae-derived biodiesel has regained interest within the last decade, industrial production is still challenging for economic reasons. Besides reactor design, as well as value chain and strain...

    Holger Morschett, Lars Freier, Jannis Rohde, Wolfgang Wiechert, Eric von Lieres and Marco Oldiges

    Biotechnology for Biofuels 2017 10:26

    Published on: 31 January 2017

  9. Research

    Feasibility of CO2 mitigation and carbohydrate production by microalga Scenedesmus obliquus CNW-N used for bioethanol fermentation under outdoor conditions: effects of seasonal changes

    Although outdoor cultivation systems have been widely used for mass production of microalgae at a relatively low cost, there are still limited efforts on outdoor cultivation of carbohydrate-rich microalgae tha...

    Shih-Hsin Ho, Yi-Di Chen, Ching-Yu Chang, Yen-Ying Lai, Chun-Yen Chen, Akihiko Kondo, Nan-Qi Ren and Jo-Shu Chang

    Biotechnology for Biofuels 2017 10:27

    Published on: 31 January 2017

  10. Research

    Exploring eukaryotic formate metabolisms to enhance microbial growth and lipid accumulation

    C1 substrates (such as formate and methanol) are promising feedstock for biochemical/biofuel production. Numerous studies have been focusing on engineering heterologous pathways to incorporate C1 substrates in...

    Zhiguo Liu, Tolutola Oyetunde, Whitney D. Hollinshead, Anna Hermanns, Yinjie J. Tang, Wei Liao and Yan Liu

    Biotechnology for Biofuels 2017 10:22

    Published on: 26 January 2017

  11. Research

    Well-to-wake analysis of ethanol-to-jet and sugar-to-jet pathways

    To reduce the environmental impacts of the aviation sector as air traffic grows steadily, the aviation industry has paid increasing attention to bio-based alternative jet fuels (AJFs), which may provide lower ...

    Jeongwoo Han, Ling Tao and Michael Wang

    Biotechnology for Biofuels 2017 10:21

    Published on: 24 January 2017

  12. Research

    T-6b allocates more assimilation product for oil synthesis and less for polysaccharide synthesis during the seed development of Arabidopsis thaliana

    As an Agrobacterium tumefaciens T-DNA oncogene, T-6b induces the development of tumors and the enation syndrome in vegetative tissues of transgenic plants. Most of these effects are related to increases in solubl...

    Yunkai Jin, Jia Hu, Xun Liu, Ying Ruan, Chuanxin Sun and Chunlin Liu

    Biotechnology for Biofuels 2017 10:19

    Published on: 21 January 2017

  13. Research

    Synthetic redesign of Escherichia coli for cadaverine production from galactose

    With increasing concerns over the environment, biological production of cadaverine has been suggested as an alternative route to replace polyamides generated from the petroleum-based process. For an ideal biop...

    Dong Hun Kwak, Hyun Gyu Lim, Jina Yang, Sang Woo Seo and Gyoo Yeol Jung

    Biotechnology for Biofuels 2017 10:20

    Published on: 21 January 2017

  14. Research

    Stochastic techno-economic analysis of alcohol-to-jet fuel production

    Alcohol-to-jet (ATJ) is one of the technical feasible biofuel technologies. It produces jet fuel from sugary, starchy, and lignocellulosic biomass, such as sugarcane, corn grain, and switchgrass, via fermentat...

    Guolin Yao, Mark D. Staples, Robert Malina and Wallace E. Tyner

    Biotechnology for Biofuels 2017 10:18

    Published on: 19 January 2017

  15. Research

    Identification and characterization of the glucose dual-affinity transport system in Neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression

    The glucose dual-affinity transport system (low- and high-affinity) is a conserved strategy used by microorganisms to cope with natural fluctuations in nutrient availability in the environment. The glucose-sen...

    Bang Wang, Jingen Li, Jingfang Gao, Pengli Cai, Xiaoyun Han and Chaoguang Tian

    Biotechnology for Biofuels 2017 10:17

    Published on: 19 January 2017

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