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  1. Research

    Photoautotrophic production of polyhydroxyalkanoates in a synthetic mixed culture of Synechococcus elongatus cscB and Pseudomonas putida cscAB

    One of the major challenges for the present and future generations is to find suitable substitutes for the fossil resources we rely on today. Cyanobacterial carbohydrates have been discussed as an emerging ren...

    Hannes Löwe, Karina Hobmeier, Manuel Moos, Andreas Kremling and Katharina Pflüger-Grau

    Biotechnology for Biofuels 2017 10:190

    Published on: 19 July 2017

  2. Research

    Towards microalgal triglycerides in the commodity markets

    Microalgal triglycerides (TAGs) hold great promise as sustainable feedstock for commodity industries. However, to determine research priorities and support business decisions, solid techno-economic studies are...

    Giulia Benvenuti, Jesús Ruiz, Packo P. Lamers, Rouke Bosma, René H. Wijffels and Maria J. Barbosa

    Biotechnology for Biofuels 2017 10:188

    Published on: 17 July 2017

  3. Research

    Highly efficient methane generation from untreated microalgae biomass

    The fact that microalgae perform very efficiently photosynthetic conversion of sunlight into chemical energy has moved them into the focus of regenerative fuel research. Especially, biogas generation via anaer...

    Viktor Klassen, Olga Blifernez-Klassen, Daniel Wibberg, Anika Winkler, Jörn Kalinowski, Clemens Posten and Olaf Kruse

    Biotechnology for Biofuels 2017 10:186

    Published on: 17 July 2017

  4. Research

    Heterologous co-expression of a yeast diacylglycerol acyltransferase (ScDGA1) and a plant oleosin (AtOLEO3) as an efficient tool for enhancing triacylglycerol accumulation in the marine diatom Phaeodactylum tricornutum

    Microalgae are promising alternate and renewable sources for producing valuable products such as biofuel and essential fatty acids. Although this is the case, there are still challenges impeding on the effecti...

    Nodumo Nokulunga Zulu, Jennifer Popko, Krzysztof Zienkiewicz, Pablo Tarazona, Cornelia Herrfurth and Ivo Feussner

    Biotechnology for Biofuels 2017 10:187

    Published on: 17 July 2017

  5. Research

    Identification of developmental stage and anatomical fraction contributions to cell wall recalcitrance in switchgrass

    Heterogeneity within herbaceous biomass can present important challenges for processing feedstocks to cellulosic biofuels. Alterations to cell wall composition and organization during plant growth represent ma...

    Jacob D. Crowe, Nicholas Feringa, Sivakumar Pattathil, Brian Merritt, Cliff Foster, Dayna Dines, Rebecca G. Ong and David B. Hodge

    Biotechnology for Biofuels 2017 10:184

    Published on: 15 July 2017

  6. Research

    Two bifunctional enzymes from the marine protist Thraustochytrium roseum: biochemical characterization of wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis

    Triacylglycerols (TAGs) and wax esters (WEs) are important neutral lipids which serve as energy reservoir in some plants and microorganisms. In recent years, these biologically produced neutral lipids have bee...

    Nannan Zhang, Zejing Mao, Ling Luo, Xia Wan, Fenghong Huang and Yangmin Gong

    Biotechnology for Biofuels 2017 10:185

    Published on: 15 July 2017

  7. Methodology

    Comparison of four glycosyl residue composition methods for effectiveness in detecting sugars from cell walls of dicot and grass tissues

    The effective use of plant biomass for biofuel and bioproduct production requires a comprehensive glycosyl residue composition analysis to understand the different cell wall polysaccharides present in the diff...

    Ajaya K. Biswal, Li Tan, Melani A. Atmodjo, Jaclyn DeMartini, Ivana Gelineo-Albersheim, Kimberly Hunt, Ian M. Black, Sushree S. Mohanty, David Ryno, Charles E. Wyman and Debra Mohnen

    Biotechnology for Biofuels 2017 10:182

    Published on: 14 July 2017

  8. Research

    Enzymatic degradation of sulfite-pulped softwoods and the role of LPMOs

    Recent advances in the development of enzyme cocktails for degradation of lignocellulosic biomass, especially the discovery of lytic polysaccharide monooxygenases (LPMOs), have opened new perspectives for proc...

    Piotr Chylenski, Dejan M. Petrović, Gerdt Müller, Marie Dahlström, Oskar Bengtsson, Martin Lersch, Matti Siika-aho, Svein Jarle Horn and Vincent G. H. Eijsink

    Biotechnology for Biofuels 2017 10:177

    Published on: 11 July 2017

  9. Research

    Modification of the nanostructure of lignocellulose cell walls via a non-enzymatic lignocellulose deconstruction system in brown rot wood-decay fungi

    Wood decayed by brown rot fungi and wood treated with the chelator-mediated Fenton (CMF) reaction, either alone or together with a cellulose enzyme cocktail, was analyzed by small angle neutron scattering (SAN...

    Barry Goodell, Yuan Zhu, Seong Kim, Kabindra Kafle, Daniel Eastwood, Geoffrey Daniel, Jody Jellison, Makoto Yoshida, Leslie Groom, Sai Venkatesh Pingali and Hugh O’Neill

    Biotechnology for Biofuels 2017 10:179

    Published on: 11 July 2017

  10. Research

    Limitation of cellulose accessibility and unproductive binding of cellulases by pretreated sugarcane bagasse lignin

    The effectiveness of the enzymatic hydrolysis of cellulose in plant cell wall is strongly influenced by the access of enzymes to cellulose, which is at least in part limited by the presence of lignin. Although...

    Germano Siqueira, Valdeir Arantes, Jack N. Saddler, André Ferraz and Adriane M. F. Milagres

    Biotechnology for Biofuels 2017 10:176

    Published on: 11 July 2017

  11. Research

    Electricity-assisted production of caproic acid from grass

    Medium chain carboxylic acids, such as caproic acid, are conventionally produced from food materials. Caproic acid can be produced through fermentation by the reverse β-oxidation of lactic acid, generated from...

    Way Cern Khor, Stephen Andersen, Han Vervaeren and Korneel Rabaey

    Biotechnology for Biofuels 2017 10:180

    Published on: 11 July 2017

  12. Research

    Transcriptome and key genes expression related to carbon fixation pathways in Chlorella PY-ZU1 cells and their growth under high concentrations of CO2

    The biomass yield of Chlorella PY-ZU1 drastically increased when cultivated under high CO2 condition compared with that cultivated under air condition. However, less attention has been given to the microalgae pho...

    Yun Huang, Jun Cheng, Hongxiang Lu, Yong He, Junhu Zhou and Kefa Cen

    Biotechnology for Biofuels 2017 10:181

    Published on: 11 July 2017

  13. Research

    A type-I diacylglycerol acyltransferase modulates triacylglycerol biosynthesis and fatty acid composition in the oleaginous microalga, Nannochloropsis oceanica

    Photosynthetic oleaginous microalgae are considered promising feedstocks for biofuels. The marine microalga, Nannochloropsis oceanica, has been attracting ever-increasing interest because of its fast growth, high...

    Hehong Wei, Ying Shi, Xiaonian Ma, Yufang Pan, Hanhua Hu, Yantao Li, Ming Luo, Henri Gerken and Jin Liu

    Biotechnology for Biofuels 2017 10:174

    Published on: 5 July 2017

  14. Research

    A novel Sugarcane bacilliform virus promoter confers gene expression preferentially in the vascular bundle and storage parenchyma of the sugarcane culm

    Saccharum species such as sugarcane and energy cane are key players in the expanding bioeconomy for sugars, bioenergy, and production of high-value proteins. Genomic tools such as culm...

    San-Ji Gao, Mona B. Damaj, Jong-Won Park, Xiao-Bin Wu, Sheng-Ren Sun, Ru-Kai Chen and T. Erik Mirkov

    Biotechnology for Biofuels 2017 10:172

    Published on: 4 July 2017

  15. Research

    From grass to gas: microbiome dynamics of grass biomass acidification under mesophilic and thermophilic temperatures

    Separating acidification and methanogenic steps in anaerobic digestion processes can help to optimize the process and contribute to producing valuable sub-products such as methane, hydrogen and organic acids. ...

    Christian Abendroth, Claudia Simeonov, Juli Peretó, Oreto Antúnez, Raquel Gavidia, Olaf Luschnig and Manuel Porcar

    Biotechnology for Biofuels 2017 10:171

    Published on: 3 July 2017

  16. Research

    Integrated analysis of transcriptome and metabolites reveals an essential role of metabolic flux in starch accumulation under nitrogen starvation in duckweed

    Duckweed is considered a promising source of energy due to its high starch content and rapid growth rate. Starch accumulation in duckweed involves complex processes that depend on the balanced expression of ge...

    Changjiang Yu, Xiaowen Zhao, Guang Qi, Zetao Bai, Yu Wang, Shumin Wang, Yubin Ma, Qian Liu, Ruibo Hu and Gongke Zhou

    Biotechnology for Biofuels 2017 10:167

    Published on: 26 June 2017

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