Measurement of saccharifying cellulase

Table 3 Filter Paper (FP) Activity and Units^a

International units/ml	Filter	Paper	Activity		ml enzyme for 2.0 mg glucose
	1.0 ml	0.5 ml	0.2 ml	0.1 ml
0.05	0.54	0.27	-	-	-
0.10	1.08	0.54	-	-	-
0.20	2.10	1.08	0.40	-	0.925
0.30	2.50	1.62	0.80	-	0.615
0.40	2.80	2.10	1.10	0.40	0.463
0.50	3.00	2.30	1.40	0.80	0.370
0.75	3.90	2.80	1.75	1.10	0.248
1.00	4.60	3.00	2.10	1.40	0.185
1.50	-	3.90	2.65	1.75	0.124
2.00	-	4.60	3.20	2.10	0.093

^aSubstrate: 50 mg Whatman No. 1 filter paper (6 cm²). Buffer: 1.0 ml, 0.05 M citrate pH 4.8. Enzyme: 1 ml assay, volume indicated + buffer to 1.0 ml FP activity × 0.0925 = IU/ml; 0.5 ml assay volume indicated + buffer to 0.5 ml FP activity × 0.185 IU/ml or if FP activity is greater than 2.0 IU/ml = 0.185/ml enzyme for 2.0 mg glucose. (2.0 mg glucose = 4% hydrolysis of 50 mg; the unit based on 0.5 mg of glucose from 1 ml of enzyme [8] is equal to 0.0463 IU.)

ISSN: 2731-3654