Table 2 Primers used in this study.
Primer | Sequence | Restriction Endonuclease |
|---|---|---|
5XYL1s | 5'-GCGGATCC TCTAGA ATGCCTT-3' | Bam HI |
3XYL1s | 5'-TTGGATCC TCTAGA TTAGACGAAG-3' | Bam HI |
5K270R | 5'-CATCATTCCAAGG TCCAACACTG-3' | |
3K270R | 5'-CAGTGTTGGACCT TGGAATGATG-3' | |
pY7-XR-for | 5'-GCAAGCTT GGCGCGCC GGGATCGAAGAAATGATGG-3' | Hind III, Asc I |
pY7-XR-rev | 5'-CGCGCGCGCTGCAG GTGTGGAAGAACGATTACAAC-3' | Pst I |
pY7-XDH-for | 5'-GCCTGCAG TCTAACTGATCTATCCAAAACTG-3' | Pst I |
pY7-XDH-rev | 5'-CGTGAGCTC CGTACG TAACGAACGCAGAATTTTC-3' | Sac I, Bsi WI |