Rapid optimization of enzyme mixtures for deconstruction of diverse pretreatment/biomass feedstock combinations

Table 2 Optimized proportions of six core enzymes for release of Glc from 15 pretreatment/substrate combinations*

		Optimized enzyme proportions (%)						Glc yield (%)
Feedstock	Pre-treatment	CBH1	BG	EG1	BX	EX3	CBH2	MP	Exptl.
Corn stover	AFEX	35	12	26	4	19	4	42.9	43.9 ± 0.5
	0.25% NaOH	49	5	34	4	4	4	42.0	40.7 ± 1.0
	Alk. peroxide	43	8	30	4	11	4	58.9	58.2 ± 0.2
Switchgrass	AFEX	31	4	23	4	35	4	23.0	24.4 ± 0.6
	0.25% NaOH	46	12	30	4	4	4	27.0	25.9 ± 0.8
	Alk. peroxide	47	4	19	4	22	4	38.0	39.1 ± 0.5
Miscanthus	AFEX	42	4	42	4	4	4	23.0	22.8 ± 1.3
	0.25% NaOH	36	4	46	4	5	4	17.0	17.5 ± 0.7
	Alk. peroxide	48	4	32	4	7	4	30.0	32.1 ± 1.2
DDGS	AFEX	28	20	23	4	20	4	21.3	22.6 ± 0.8
	0.25% NaOH	27	27	34	4	4	4	23.1	23.8 ± 0.5
	Alk. peroxide	16	43	29	4	4	4	31.0	29.8 ± 0.5
Poplar	AFEX	44	4	40	4	4	4	14.5	13.8 ± 0.1
	0.25% NaOH	47	4	36	4	4	4	10.0	9.8 ± 0.1
	Alk. peroxide	47	4	37	4	4	4	10.0	10.5 ± 0.2

*MP, model prediction. Exptl., experimental results. Data are expressed as means ± 1 SD (n = 8). All enzyme loadings were 15 mg/g glucan.

ISSN: 2731-3654