From: A high-throughput transient gene expression system for switchgrass (Panicum virgatum L.) seedlings
Treatment* | GUS-positive seedlings | ||
---|---|---|---|
Total, n* | Mean ± SE† | % | |
Control-1 (no vacuum; no separation by centrifugation) | 17 | 4.2 ± 0.6b | 8.5 |
Control-2 (no vacuum; separation by centrifugation) | 43 | 10.8 ± 2.6a | 21.5 |
Vacuum (1 minute); separation by centrifugation | 43 | 10.8 ± 1.6a | 21.5 |
Vacuum (2 minutes); separation by centrifugation | 44 | 11.0 ± 2.1a | 22.0 |
Vacuum (4 minutes); separation by centrifugation | 49 | 12.2 ± 3.2a | 24.5 |
Vacuum (8 minutes); separation by centrifugation | 50 | 12.5 ± 0.5a | 25.0 |
Vacuum (16 minutes); separation by centrifugation | 55 | 13.8 ± 1.4a | 27.5 |