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Table 4 A comparison of vacuum infiltration and separation by centrifugation on GUSPlus expression in sonicated switchgrass seedlings.

From: A high-throughput transient gene expression system for switchgrass (Panicum virgatum L.) seedlings

Treatment*

GUS-positive seedlings

 

Total, n*

Mean ± SE†

%

Control-1 (no vacuum; no separation by centrifugation)

17

4.2 ± 0.6b

8.5

Control-2 (no vacuum; separation by centrifugation)

43

10.8 ± 2.6a

21.5

Vacuum (1 minute); separation by centrifugation

43

10.8 ± 1.6a

21.5

Vacuum (2 minutes); separation by centrifugation

44

11.0 ± 2.1a

22.0

Vacuum (4 minutes); separation by centrifugation

49

12.2 ± 3.2a

24.5

Vacuum (8 minutes); separation by centrifugation

50

12.5 ± 0.5a

25.0

Vacuum (16 minutes); separation by centrifugation

55

13.8 ± 1.4a

27.5

  1. + All seedlings were sonicated for 1 minute.
  2. *Three replicates of 50 seedlings per replicate were tested in each treatment.
  3. †Means followed by the same letter (a, b, c) were not significantly different at the 5% level as determined by The Tukey multiple comparison test.