Table 3 Buffers for the pH optimum assay
From: Bioprospecting metagenomics of decaying wood: mining for new glycoside hydrolases
pH | Buffer |
|---|---|
pH 4.5 | 50 mM Citrate, 100 mM NaCl |
pH 5 | 50 mM Acetate, 100 mM NaCl |
pH 5.5 | 50 mM Acetate, 100 mM NaCl |
pH 6 | 50 mM Phosphate, 100 mM NaCl |
pH 6.5 | 50 mM Phosphate, 100 mM NaCl |
pH 7 | 50 mM Phosphate, 100 mM NaCl |
pH 7.5 | 50 mM Phosphate, 100 mM NaCl |
pH 8 | 50 mM Tris, 100 mM NaCl |