Figure 2

Northern blot analysis of E1 gene transcript in the leaves. Twenty-five micrograms of total RNA, extracted from the leaves of six independent T0 transgenic lines (#1 to #6) and wild type (WT) rice plants, was loaded into each lane, transferred to nylon membrane after electrophoresis and probed with (A) E1 DNA or (B) stained for rRNA by EtBr. Note that no E1 gene transcript was detected in transgenic line #4 and wild type plants.