Tracking dynamics of plant biomass composting by changes in substrate structure, microbial community, and enzyme activity

Table 3 Subgenus- and species-level primers used to determine the transcriptional levels of fungal cellulolytic genes in biomass compost

Enzyme type/core species	Target gene/enzyme	Genbank accession No. or reference	Primer sequences [I]	Amplicon size (bp)
Trichoderma spp.	ITS	-	F: TACCAAHCTGTTGCCTCGGCGG	~200
			R: GATGAAGAAGGCAGCGAAATGCGATA
Cellulase/Trichoderma spp.	cbh 1/Cel7 A	[II]	F: GATGATTACTACGCCAACATGCTG	77
	bgl 1/Cel3 A	[IV]	F: ATCAAGGTAGCTCAACATCGGG	124
			R: ACCTTATCTTGGAGATTGAGCTTTGCC
Ligninase/Phanerochaete chrysosporium	LipA/B	M37701	F: ATCTCTGCCCACCCTGTCCT	256
			R: CTGAGCCAGCGAATGAGAGTC
	LipH	M24082	F: CGTCCACGGATATCGCTCTCT	102
			R: GCGAGGGAGACGCAAATTC
			R: TGTTCGGGTGGAAATTGGTC

Note: the group primers targeting specific functional protein-encoding genes:
[I]. Orpinomyces spp. endo-1,4-glucanase gene: O. sp. PC-2, U97153; O. joyonii, AF015248.
[II]. Trichoderma spp. cellobiohydrolase I gene (cbh 1): T. koningii, X69976; T. reesei, DD393553-DD393571; T. sp. FJ026620; T. viride, AY368686.
[III]. Trichoderma spp. endo-β-1,4-glucanase I gene (egl 1): T. longibrachiatum, X60652; T. reesei, AY928809; T. viride, AY343986
[IV]. Trichoderma spp. β-glucosidase 1 gene (bgl 1): T. reesei, U09580; T. sp. SSL, FJ040193; T. viride, AY368687
[V]. Trichoderma spp. xyn 1 and xyn 2: T.reesei, X69573 (xyn 1), U24191 (xyn 2); T. harzianum, EU821597 (xyn 2); T. pseudokoningii, EU360941 (xyn 2).

ISSN: 2731-3654