Table 1 Number of transformants isolated when E. coli methylation is varied
From: Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum
|  |  | Average number of transformants per µg DNA (range) | |
|---|---|---|---|
E. colihosta | Methylationb | pAMG205 | pAMG206 |
Top10 | Dam+, Dcm+ | 4 (0−14) | 570 (95−630) |
C2925 | Dam-, Dcm- | 1 (0−4) | 1 (0−2) |
C2925+i.v. Damc | Dam+, Dcm- | 2200 (960−4800) | 1900 (820−4200) |
BL21 | Dam+, Dcm- | 2600 (680−5900) | 4400 (1100−9500) |