Figure 5From: Cytosolic re-localization and optimization of valine synthesis and catabolism enables increased isobutanol production with the yeast Saccharomyces cerevisiaeKDC activities. Strain Isoy21 was transformed with plasmids overexpressing ARO10 of S. cerevisiae, kivD of Lactococcus lactis or the empty vector. The wild-type strain CEN.PK2-1C was transformed with the empty vector. Transformants were grown in selective SCD medium and KDC activity determined in crude extracts and normalized to the amount of protein.Back to article page