Figure 1From: Production of four Neurospora crassa lytic polysaccharide monooxygenases in Pichia pastoris monitored by a fluorimetric assaySchematic representation of the PMO activity assay. Ascorbate or CDH reduce the type-2 copper in the PMO, which activates molecular oxygen by a one electron reduction. The released H2O2 is detected by the HRP coupled conversion of Amplex Red to resurofin.Back to article page