Figure 7From: Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824Western blot analysis of cellulosomal operons secreted by the recombinant C. acetobutylicum strains. The wild type (wt, C. acetobutylicum ATCC 824) and the recombinant strains (thlOIDCel9B, thlOIDCel8A, thlOIDCel9B_CipA3, thlOIDCel8A_CipA3, thlOIDCel9B_Cel8A_CipA3, thlOIDCipA3) were grown in buffered 2xYTG (pH 7 using 40 mM MOPS) until early exponential phase (OD600 0.8), concentrated 50-fold and separated by SDS-PAGE. All heterologous proteins were detected using ANTI-FLAG M2 monoclonal antibody-horseradish peroxidise conjugate (Sigma). M, ColorPlus Prestained Protein Ladder (10–230 kDa; New England Biolabs); Flag, N-Flag-Bap control protein (50 kDa, 50 ng/lane; Sigma). Expected molecular masses are indicated by arrows.Back to article page