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Table 1 Functional classification and quantitation of the same proteins identified in different treatments

From: Secretome diversity and quantitative analysis of cellulolytic Aspergillus fumigatusZ5 in the presence of different carbon sources

Accession no.

Name of proteins

LIBER number peptides

num unique peps

tot indep spectra

percent share of spectrum id's

iTRAQ Ratio

116/119

117/119

Family

Signal P

116

117

119

Cellulase

gi|66844580

glycosyl hydrolase family 43 protein

4

1

7

0.21

0.16±0.03

0.11±0.01

0.03±0.01

5.33

3.67

GH 43 (PF04616)

Y

gi|66845136

beta-1,6-glucanase Neg1

2

1

2

0.13

0.17±0.02

0.47±0.02

0.10±0

1.70

4.70

GH 30 (PF02055)

Y

gi|66845978

beta-1,3-endoglucanase EglC

25

2

29

1.89

0.04±0.01

0.63±0.03

0.11±0.01

0.36

5.73

GH 17 (PF00332)

Y

gi|66846140

cellobiohydrolase

10

1

12

0.76

0.19±0.02

0.10±0.01

0.09±0.01

2.11

1.11

GH 6 (PF01341)

Y

gi|66846336

endo-1,4-beta-glucanase

10

3

10

0.64

0.24±0.01

0.09±0.02

0.13±0.02

1.85

0.69

GH 61 (PF03443)

Y

gi|66846525

endoglucanase

6

2

6

0.39

0.27±0.03

0.14±0.01

0.19±0.04

1.42

0.74

GH 12 (PF01670)

Y

gi|66846526

beta-D-glucoside glucohydrolase

13

2

15

0.97

0.28±0.01

0.16±0.01

0.11±0.01

2.55

1.45

GH 3 (PF00933)

Y

gi|66846837

glucan 1,4-alpha-glucosidase

3

2

1

0.13

0.31±0.04

0.18±0.09

0.05±0.01

6.20

3.60

GH 15 (PF00723)

Y

gi|66846860

extracellular glycosyl hydrolase

1

1

1

0.09

0.17±0.03

0.15±0.01

0.07±0.01

2.43

2.14

GH 62 (PF03664)

Y

gi|66848676

endoglucanase

2

1

2

0.13

0.33±0.07

0.10±0

0.03±0

11.00

3.33

GH 5 (PF00150)

Y

gi|66849674

endoglucanase

58

15

109

6.98

0.31±0.01

0.12±0

0.04±0

7.75

3.00

GH 61 (PM03443)

Y

gi|66850620

extracellular cell wall glucanase

1

1

1

0.07

0.2±0.04

0.55±0.07

0.06±0

3.33

9.17

GH 16 (PF00722)

Y

gi|66850743

beta-glucosidase

1

2

1

0.15

0.42±0

0.26±0.02

0.06±0

7.00

4.33

GH 3 (PF01915)

Y

gi|74670557

1,4-beta-D-glucan-cellobiohydrolyase

21

12

100

7.45

0.28±0.01

0.11±0.01

0.06±0.01

4.67

1.83

GH 7 (PF00840)

Y

gi|74670999

cellobiohydrolase

17

12

35

3.22

0.30±0.01

0.09±0.01

0.06±0.01

5.00

1.50

GH 7 (PF00840)

Y

Hemicellulase

gi|66845449

alpha-galactosidase

2

2

3

0.12

0.25±0.01

0.13±0.11

0.11±0.08

2.27

1.18

Melibiase (PF02065)

N

gi|66845983

endo-1,4-beta-xylanase (XlnA)

31

9

49

3.16

0.34±0.01

0.07±0

0.02±0.01

17.00

3.50

GH 11 (PF00457)

Y

gi|66846833

arabinosidase

1

2

2

0.08

0.44±0.09

0.10±0

0.03±0

14.67

3.33

GH 43 (PF04616)

N

gi|66846861

xylosidase

1

2

4

0.20

0.72±0

0.21±0

0.01±0

72.00

21.00

GH 43 (PF04616)

Y

gi|66848870

endo-1,4-beta-xylanase

21

7

35

2.27

0.35±0.01

0.08±0.01

0.05±0.01

7.00

1.60

GH 10 ( PF00331 )

Y

gi|66850460

mannosidase MsdS

1

2

6

0.09

0.44±0.07

0.06±0.01

0.13±0.02

3.38

0.46

GH 47 ( PF01532)

Y

gi|66851740

endo-1,4-beta-xylanase

2

2

3

0.12

0.35±0.02

0.13±0.05

0.11±0.08

3.18

1.18

GH 10 ( PF00331)

Y

gi|70981394

extracellular arabinanase

4

3

4

0.26

0.27±0.03

0.24±0.01

0.11±0.06

2.45

2.18

GH 43 (PF04616)

Y

gi|70994060

extracellular endo-1,4-beta-xylanase

17

10

26

1.62

0.31±0.01

0.08±0

0.06±0.01

5.17

1.33

GH 10 (PF00331)

Y

gi|74668470

endo-1,4-beta-xylanase

5

3

5

0.23

0.28±0.05

0.24±0.01

0.13±0.06

2.15

1.85

GH 11 (PF00457)

Y

Chitinase

gi|129556953

class V chitinase

1

1

1

0.09

0.27±0.03

0.10±0.01

0.35±0.07

0.77

0.29

GH18 (PF00704)

Y

gi|129558344

class V chitinase

6

8

14

0.86

0.24±0.01

0.26±0.01

0.1±0.02

2.40

2.60

GH 18 (PF00704)

Y

gi|66846625

class III chitinase

5

5

5

0.30

0.19±0.04

0.17±0.06

0.15±0.06

1.27

1.13

GH 18 (PF00704)

Y

gi|70983075

class V chitinase ChiB1

72

23

159

10.35

0.27±0.01

0.09±0

0.11±0.01

2.45

0.82

GH 18 (PF00704)

Y

Phosphatase, lipase, pectinase, lignin degrading proteins, esterase and protease

gi|129557040

Ser/Thr protein phosphatase family protein

1

2

2

0.09

0.49±0.08

0.06±0.01

0.15±0.02

3.27

0.40

Metallophos (PF00149)

N

gi|129558573

esterase

1

2

3

0.18

0.07±0

0.26±0.02

0.55±0.05

0.13

0.47

-

Y

gi|66845766

extracellular lipase

37

7

64

4.12

0.07±0.01

0.34±0.01

0.35±0.02

0.20

0.97

Lipase 3 (PF01764)

Y

gi|66846844

pectate lyase

1

1

1

0.07

0.73±0.04

0.15±0.01

0.25±0.04

2.92

0.60

Pec_lyase_C (PF00544)

Y

gi|66849090

metalloprotease

10

4

13

0.84

0.38±0.07

0.1±0.03

0.07±0.04

5.43

1.43

Peptidase_M35 (MF02102)

Y

gi|66853735

cellobiose dehydrogenase

10

3

12

0.79

0.16±0.02

0.41±0.03

0.19±0.02

0.84

2.16

GMC_oxred_N (PF00732)

Y

gi|70986104

CAT1 mycelial catalase Cat1

8

5

10

0.60

0.21±0.02

0.09±0.01

0.25±0.02

0.84

0.36

Catalase (PF00199)

N

gi|70997966

Cu, Zn superoxide dismutase

4

1

4

0.26

0.18±0.03

0.19±0.01

0.18±0.01

1.00

1.06

Sod_Cu (PF00080)

N

Permease, reductase, helicase and Transporter

gi|66844481

high affinity methionine permease

2

1

2

0.13

0.28±0.01

0.19±0.02

0.17±0.02

1.65

1.12

AA_permease2 ( PF13520)

N

gi|66848496

thioredoxin reductase GliT

2

4

4

0.23

0.14±0.08

0.37±0.06

0.29±0.01

0.48

1.28

Pyr_redox_2 (PF07992)

N

gi|66848977

telomere-associated RecQ helicase

31

3

52

3.16

0.01±0

0.47±0

0.46±0.01

0.02

1.02

DUF3505 (PF12013)

N

gi|70982865

MFS transporter

8

3

47

2.40

0.26±0

0.29±0.01

0.15±0

1.73

1.93

MFS_1 (PF07690)

N

Other proteins

gi|129555482

cytochrome P450

5

2

5

0.29

0.11±0.03

0.27±0.03

0.31±0.10

0.35

0.87

p450 (PF00067)

N

gi|129555486

secreted antimicrobial peptide

3

2

3

0.16

0.10±0.04

0.51±0.09

0.20±0.01

0.50

2.55

MiAMP1 (PF09117)

Y

gi|146324637

hybrid PKS-NRPS enzyme

2

2

3

0.13

0.18±0.02

0.16±0.06

0.32±0.02

0.56

0.50

Acyl_transf_1 (PF00698)

N

gi|66847207

HECT domain protein

5

3

8

0.51

0.29±0.03

0.12±0.01

0.15±0.02

1.93

0.80

HECT (PF00632)

N

gi|66847547

C6 finger domain protein

2

1

2

0.13

0.02±0

0.51±0

0.39±0.02

0.05

1.31

Zn_clus (PF00172)

N

gi|66851935

aldose 1-epimerase

2

2

13

0.11

0.26±0.04

0.16±0.06

0.32±0.07

0.81

0.50

Aldose-epim (PF01263)

N

gi|66852539

glutaminase GtaA

2

2

3

0.18

0.40±0.01

0.03±0

0.07±0

5.71

0.43

DUF1793 (PF08760)

Y

gi|66853400

translation initiation factor eIF-2B

49

1

64

3.95

0.30±0

0.27±0

0.09±0

3.33

3.00

W2 (PF02020)

N

  1. The peptides from RS, Av and Gl were labeled with the iTRAQ tags 116, 117 and 119, respectively.