Re-evaluation of glycerol utilization in Saccharomyces cerevisiae: characterization of an isolate that grows on glycerol without supporting supplements

Table 3 PCR primers used to generate the cassettes for deleting STL1 , GUT1, and GUT2 in CBS 6412-13A and for the PCR-based verification of the respective deletions

Gene	Primer	Sequence (5′-3′)
STL1	^astl1-loxP-fw	CACTCATAGTATATAAACAAGCCCTTTATTGATTTTGAATAATTAcagctgaagcttcgtacgc
	^astl1-loxP-rv	TCAAAGCCCTCTGAAGATTTTGGGACCTGCCTCTGGAGAACAAACgcataggccactagtggatctg
	^bverif-stl1∆-fw	TGGTTCACCTTTGATAGGGC
	^bverif-stl1∆-rv	TGAAACTGCTTGACCTGTGG
GUT1	^agut1-loxP-fw	TGTGGGGGGATGCCTGTTCTCGAACCATATAAAATATACCATGTGcagctgaagcttcgtacgc
	^agut1-loxP-rv	CTAGATCTCGCAGTACTGTTTTTGGCGAATCGTGTAGCTTTTCCAgcataggccactagtggatctg
	^bverif-gut1∆-fw	GTGTGGAGTAGCATAGTGAGG
	^bverif-gut1∆-rv	AATGCTAGAGTCGTCAGTGCG
GUT2	^agut2-loxP-fw	GTCTAAAGCAAGGACTCTCCCTCCCTTATCTTGACCGTGCTATTGcagctgaagcttcgtacgc
	^agut2-loxP-rv	TTGCAAAATGGCGTCACTGTATGGGCCCGTGGCATTGACCACACAgcataggccactagtggatctg
	^bverif-gut2∆-fw	TCCGATACGTTATCCACCCAA
	^bverif-gut2∆-rv	TTCCTCAGCCATTTGTCTGT

^aGene deletion cassettes; sequences complementary to the loxP regions within the disruption cassettes are shown in lower case.
^bVerification of gene deletions.

ISSN: 2731-3654