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Table 2 Effects of alkaline treatment on monosaccharides and ethanol production

From: Bioconversion of lignocellulose: inhibitors and detoxification

System studied

Detoxification conditions

Improvement in fermentability

Effect on inhibitors and sugar

Reference

Spruce hydrolysate. S. cerevisiae

Ca(OH)2 pH 10, 1 h

BEYa= 98%

Furan aldehydes, decrease: ~21%

[33]

  

BEYa (untreatedb)= 71%

Phenols, decrease: ~19%

 
  

BEYa (referencec)= 100%

Sugar, decrease: ~4%d

 
 

NaOH pH 10, 1 h

BEYa= 94%

Furan aldehydes, decrease: ~18%

 
  

BEYa (untreatedb)= 71%

Phenols, decrease: ~18%

 
  

BEYa (referencec)= 100%

Sugar, decrease: ~4%d

 

Bagasse hydrolysate. E. coli

Ca(OH)2 pH 9, 60°C, 0.5 h

Q (24 h)e: ~1.3 g/Lh

Furan aldehydes, decrease: ~69%

[97]

  

No reference fermentation

Phenols, decrease: ~35%

 
   

Sugar, decrease: ~15%f

 
 

Ca(OH)2 pH 10, 60°C, 0.5 h

Q(24 h)e: ~ 1.0 g/Lh

Sugar, decrease: ~33%f

 
  

No reference fermentation

  

Spruce hydrolysate. S. cerevisiae

Ca(OH)2 pH 12, 60°C, 170 h

Q(24 h)e: ~ 0.3 g/Lh

Furan aldehydes, decrease: ~100%

[100]

  

No reference fermentation

Phenols, increase: ~150%

 
   

Sugar, decrease: ~68%g

 
 

Ca(OH)2 pH 11, 25°C, 20 h

Q(24 h)e: ~ 0 g/Lh

Furan aldehydes, decrease: ~77%

 
  

Q(48 h)e: ~ 0.3 g/Lh

Phenols, decrease: ~8%

 
  

No reference fermentation

Sugar, decrease: <5%g

 

Bagasse hydrolysate. S. cerevisiae

Ca(OH)2 pH 10, 1 h

BEYa= 92%

Furan aldehydes, decrease: >25%

[43]

  

BEYa (untreatedb)= 68%

Phenols, decrease: ~17%

 
  

BEYa (referencec)= 100%

Sugar, decrease: ~1%f

 

Spruce hydrolysate. S. cerevisiae

Ca(OH)2 pH 11, 30°C, 3 h

BEYa= 120%

Furan aldehydes, decrease: ~59%

[49]

  

BEYa (untreatedb)= 5%

Phenols, decrease: ~22%

 
  

BEYa (referencec)= 100%

Sugar, decrease: ~14%h

 

Spruce hydrolysate. S. cerevisiae

NH4OH pH 10, 22°C, 3 h

BEYa =110%

Not determined

[76]

  

BEYa (untreatedb)= 10%

  
  

BEYa (referencec)= 100%

  

Spruce hydrolysate. S. cerevisiae

NaOH pH 9, 55°C, 3 h

BEYa = 111%

Furan aldehydes, decrease: ~33%

[77]

  

BEYa (untreatedb) = 6%

Phenols, decrease: ~12%

 
  

BEYa (referencec) = 100%

Sugar, decrease: ~9%d

 
 

NH4OH pH 9, 55°C, 3 h

BEYa =120%

Furan aldehydes, decrease: ~33%

 
  

BEYa (untreatedb) = 7%

Phenols, decrease: ~13%

 
  

BEYa (referencec) = 100%

Sugar, decrease: ~7%d

 

Corn stover hydrolysate. Z. mobilis

Ca(OH)2 pH 9, 50°C, 0.5 h

No reference fermentation. OEYi = 62%

Sugar, decrease: ~7%j

[101]

 

Ca(OH)2 pH 10, 50°C, 0.5 h

No reference fermentation. OEYi = 70%.

Sugar, decrease: ~13%j

 
 

Ca(OH)2 pH 11, 50°C, 0.5 h

No reference fermentation. OEYi = 59%

Sugar, decrease: ~29%j

 
  1. a Balanced ethanol yield given in percent of a reference fermentation of a sugar solution. b Untreated hydrolysate. c Reference sugar solution. d Glucose, xylose, arabinose, galactose mannose, and cellobiose. e Ethanol productivity. f Glucose, xylose, arabinose, galactose, and mannose. g Glucose, xylose, galactose, and mannose. h Glucose and mannose. i Overall ethanol yield, yield calculated on sugars present prior to detoxification, given in percent of the theoretical yield. j Glucose, xylose, and arabinose.