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Table 2 Experimental design and results obtained in the detoxification treatment and during the fermentation of hydrolysate treated

From: Treatment of rice straw hemicellulosic hydrolysates with advanced oxidative processes: a new and promising detoxification method to improve the bioconversion process

Assays

Experimental conditions (Real values)

Reduction (%)

QP** (%)

A

B

AB

C

AC

BC

DE

D

AD

BD

CE

CD

BE

AE

E

Furans

LMWPC

Total phenolics

Fe+2(mg/L)

H2O2(mg/L)

 

UV

*

  

O3(mg/L)

*

 

*

 

*

*

pH

    

A1

2 (50)

2 (1000)

2

2 (present)

2

2

1

2 (500)

2

2

1

2

1

1

1 (pH 3)

44

70

35

7

A2

2 (50)

2 (1000)

2

2 (present)

2

2

1

1 (0)

1

1

2

1

2

2

2 (pH 8)

3

23

1

49

A3

2 (50)

2 (1000)

2

1

1

1

2

2 (500)

2

2

1

1

2

2

2 (pH 8)

52

95

40

99

A4

2 (50)

2 (1000)

2

1

1

1

2

1 (0)

1

1

2

2

1

1

1 (pH 3)

11

16

−10

27

A5

2 (50)

1 (0)

1

2 (present)

2

1

2

2 (500)

2

1

2

2

1

2

2 (pH 8)

55

96

47

98

A6

2 (50)

1 (0)

1

2 (present)

2

1

2

1 (0)

1

2

1

1

2

1

1 (pH 3)

14

16

4

29

A7

2 (50)

1 (0)

1

1

1

2

1

2 (500)

2

1

2

1

2

1

1 (pH 3)

33

60

43

50

A8

2 (50)

1 (0)

1

1

1

2

1

1 (0)

1

2

1

2

1

2

2 (pH 8)

−3

11

−7

56

A9

1 (0)

2 (1000)

1

2 (present)

1

2

2

2 (500)

1

2

2

2

2

1

2 (pH 8)

50

95

41

66

A10

1 (0)

2 (1000)

1

2 (present)

1

2

2

1 (0)

2

1

1

1

1

2

1 (pH 3)

18

19

−12

6

A11

1 (0)

2 (1000)

1

1

2

1

1

2 (500)

1

2

2

1

1

2

1 (pH 3)

41

64

44

4

A12

1 (0)

2 (1000)

1

1

2

1

1

1 (0)

2

1

1

2

2

1

2 (pH 8)

−4

23

4

88

A13

1 (0)

1 (0)

2

2 (present)

1

1

1

2 (500)

1

1

1

2

2

2

1 (pH 3)

40

63

42

20

A14

1 (0)

1 (0)

2

2 (present)

1

1

1

1 (0)

2

2

2

1

1

1

2 (pH 8)

5

8

−11

82

A15

1 (0)

1 (0)

2

1

2

2

2

2 (500)

1

1

1

1

1

1

2 (pH 8)

54

95

44

79

A16

1 (0)

1 (0)

2

1

2

2

2

1 (0)

2

2

2

2

2

2

1 (pH 3)

−2

5

−6

32

  1. * Columns used in error estimation. ** Percentage in relation to that obtained in the semi-synthetic medium fermentation (QP = 0.36 g/L.h).
  2. Taguchi L16 experimental array, with different conditions of treatment by homogeneous AOPs evaluated, and responses: reduction of furans, low molecular weight phenolic compounds (LMWPC) and total phenols as well as the volumetric ethanol productivity by Pichia stipitis (expressed as a percentage relative to productivity obtained through the same conditions in semi-synthetic medium fermentation, QP = 0.36 g/L.h). The standard deviation was less than 5%.