A pyrosequencing-based metagenomic study of methane-producing microbial community in solid-state biogas reactor

Table 1 Comparison of DNA yield and purity among five DNA extraction protocols

Method	Parameters of DNA quantity and purify ^$
	DNA yield (ng/μl)	A₂₆₀/A₂₈₀	A₂₆₀/A₂₃₀
P	20.5	1.61	0.88
F	153.6	1.81	0.33
E	160.5	1.88	1.89
EY	121.4	1.81	1.82
S	ND	ND	ND

^$ DNA quantity and purity were measured fluorometrically based on NanoDrop. The purity parameter concerning carbohydrate, phenol and aromatic compound contaminations was calculated based on the ratio of the absorptions at 260 nm to 230 nm (A₂₆₀/A₂₃₀). To check for protein contamination, the ratio of the absorptions at 260 nm to 280 nm was evaluated (A₂₆₀/A₂₈₀). ND, not determined due to the impurity of DNA solution. The purity of DNA preparations was measured based on A₂₆₀/A₂₈₀ > 1.8 and A₂₆₀/A₂₃₀ > 1.8.

ISSN: 2731-3654