Structural reorganisation of cellulose fibrils in hydrothermally deconstructed lignocellulosic biomass and relationships with enzyme digestibility

Table 1 Compositional and X-ray data for as-received and hydrothermally processed wheat straw and eucalyptus pulp, and partially enzyme digested materials

Material	Process conditions	Compositional data (stem + leaf)			Powder WAXD data			Fibre WAXD data (stem internode)
	hydrothermal reactor temperature (°C)	weight loss (wt-%)	glucan (wt-%)	xylan (wt-%)	measured crystallinity (%)	fibril crystallinity (%)	crystal width (200) (nm)	crystal length (004) (nm)	disorientation
	hydrothermal reactor temperature (°C)	weight loss (wt-%)	glucan (wt-%)	xylan (wt-%)	measured crystallinity (%)	fibril crystallinity (%)	crystal width (200) (nm)	crystal length (004) (nm)	(004) (FWHH°)	(200) (FWHH°) narrow broad
Wheat straw	as received	0	39.0	23.0	24.5	63	2.8	12.1	15.5	17.5	71
	155	10	43.5	24.2	29.2	67	3.0
	180	14	47.4	24.1	28.6	60	3.2	11.8	14.7	19.6	108
	190	32	56.5	13.8	31.7	56	3.3
	200	40	60.0	1.8	26.1	44	3.4	11.6	15.0	20.0	112
Eucalyptus pulp	as-received	0	96.0	4.0	63.6	66	3.6
	180	4	96.5	1.2	67.7	70	4.2
	195	4	97.0	1.1	71.8	74	4.4
	200	4	96.9	1.1	72.0	74	4.6
	time of enzyme hydrolysis (hours)	glucose yield ⁺ (%)	remaining glucan (wt-%)		measured crystallinity (%)	fibril crystallinity (%)	crystal width (200) (nm)
Hydrothermally processed wheat straw (190^oC)*	0	0	59.0		35.4	56	3.3
Hydrothermally processed wheat straw (190^oC)*	6	43	45.1		28.3	58	3.4
As-received eucalyptus pulp*	0	0	96.0		60.0	69	3.6
	6	30	97.0		60.5	68	3.6

* separate experimental set; ⁺ from enzyme digestion, as proportion of yield from acid total hydrolysis.

ISSN: 2731-3654