Figure 3
From: Ethanol inducible expression of a mesophilic cellulase avoids adverse effects on plant development
Enzymatic activity of TrCel5A at different pH and temperature values using the azoCMC assay. Activity of TrCel5A was determined for pH values between 3.0 and 7.0 (A) and temperature between 20–70°C (B). The maximum activity measured for each system (at pH 4.8 and 55°C, respectively) was set to 100%. For panel A, the buffer systems are as indicated in the figure and temperature was set at 55°C. For panel B 50 mM Na-Acetate, pH 4.8 buffer was used throughout.